苦杏仁苷抑制人肺癌NCI-H1299细胞体外侵袭的机制
Inhibitions and mechanisms of amygdalin in non-small cell lung cancer NCI-H1299 cell line invasion in vitro
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摘要: 探讨天然产物苦杏仁苷对人非小细胞肺癌NCI-H1299细胞体外侵袭与转移的抑制作用及其机制。采用MTS法检测苦杏仁苷对肿瘤细胞生长的影响；采用Transwell小室和划痕试验检测苦杏仁苷对细胞侵袭和迁移能力的影响；采用Western blot和RT-PCR试验检测苦杏仁苷对MMP-2/9、integrin β1、integrin β4、整合素连接激酶(ILK)、黏附斑激酶(FAK)、p-FAK和β-catenin的表达水平，以及Akt和RICTOR的磷酸化水平的影响。结果表明:苦杏仁苷可显著抑制H1299细胞体外增殖，0.5和1 mg/mL苦杏仁苷作用H1299细胞48 h后，肿瘤细胞侵袭、迁移能力显著下降，MMP-2/9、integrin β1/4、ILK、FAK、β-catenin蛋白和mRNA表达，MMP-2/9活性以及FAK、Akt和RICTOR磷酸化水平显著下调(P均<；0.05)。Abstract: The aim of this study was to investigate the effects and mechanisms of a nature product, amygdalin, in non-small cell lung cancer NCI-H1299 cell line invasion and migration in vitro. NCI-H1299(H1299)cells were treated with amygdalin. MTS assay was employed to determine cell proliferation, transwell chamber and wound-healing assay were employed to determine invasion and migration in vitro, western blotting and RT-PCR assay were employed to determine the expression of integrin β1, integrin β4, integrin-linked kinase(ILK), focal adhesion kinase(FAK), p-FAK and β-catenin, and the phosphorylation of Akt and RICTOR. Results showed that in vitro proliferation of H1299 cells were inhibited significantly after treated with 0. 5 and 1 mg/mL amygdalin for 48 h. The invasion and migration potential was decreased. The protein and mRNA expression of MMP-2/9, integrin β1/4, ILK, FAK, β-catenin, the activity of MMP-2/9, the phosphorylation of FAK, Akt and RICTOR were all decreased significantly(all of P< 0. 05).
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Keywords:
- amygdalin /
- non-small cell lung cancer /
- metastasis /
- NCI-H1299 /
- integrin-linked kinase /
- focal adhesion kinase
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