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两种筛选玄参中清除过氧亚硝基活性成分的在线检测方法比较研究

王莹, 陈佑华, 戚进, 余伯阳

王莹, 陈佑华, 戚进, 余伯阳. 两种筛选玄参中清除过氧亚硝基活性成分的在线检测方法比较研究[J]. 中国药科大学学报, 2016, 47(5): 560-565. DOI: 10.11665/j.issn.1000-5048.20160509
引用本文: 王莹, 陈佑华, 戚进, 余伯阳. 两种筛选玄参中清除过氧亚硝基活性成分的在线检测方法比较研究[J]. 中国药科大学学报, 2016, 47(5): 560-565. DOI: 10.11665/j.issn.1000-5048.20160509
WANG Ying, CHEN Youhua, Qi Jin, YU boyang. Comparison of two new on-line methods on screening peroxynitrite scavengers in Radix Scrophulariae[J]. Journal of China Pharmaceutical University, 2016, 47(5): 560-565. DOI: 10.11665/j.issn.1000-5048.20160509
Citation: WANG Ying, CHEN Youhua, Qi Jin, YU boyang. Comparison of two new on-line methods on screening peroxynitrite scavengers in Radix Scrophulariae[J]. Journal of China Pharmaceutical University, 2016, 47(5): 560-565. DOI: 10.11665/j.issn.1000-5048.20160509

两种筛选玄参中清除过氧亚硝基活性成分的在线检测方法比较研究

Comparison of two new on-line methods on screening peroxynitrite scavengers in Radix Scrophulariae

  • 摘要: 建立柱后HPLC-DAD-(Luminol-ONOO-)-CL(方法1)和柱前ONOO--HPLC-DAD(方法2)两种简单、快速在线检测清除ONOO-的方法,将这两种方法应用于从玄参中筛选具有清除ONOO-作用的活性物质,并结合HPLC-ESI-Q-TOF MS/MS对主要的活性成分进行鉴定。方法1从玄参中共筛选出了P1(decaffeoylacteoside)、P9(acteoside)、I6(6″-O-feruloylharpagide)、P11(cis-acteoside)、P13(angoroside)等5个具有清除ONOO-作用的活性成分,方法2从玄参中筛选出了P9和P13两个具有清除ONOO-作用的活性成分。两种筛选方法各具特点:方法1需要的仪器系统较为复杂,操作简单检测灵敏度高;方法2需要的仪器简单,操作相对方法1繁琐且灵敏度低。但是筛选结果表明,方法1和方法2均可以从复杂体系中快速地筛选出具有清除ONOO-作用的活性物质,这两种方法可以为中药复杂体系中天然ONOO-清除剂的快速发现提供技术平台。
    Abstract: Two simple and efficient methods have been developed for screening and identification of natural peroxynitrite scavengers in Radix Scrophulariae (RS). Method I was based on HPLC-DAD-(luminol-peroxynitrite)-CL techniques combined with Q-TOF MS/MS analysis, while method II was based on the pre-column reaction with peroxynitrite followed by HPLC separation with Q-TOF MS/MS analysis. Five active constituents, P1(decaffeoylacteoside), P9(eoside), I6(6″-O-feruloylharpagide), P11(cis-acteoside)and P13(angoroside), were found to possess potential peroxynitrite-scavenging activity by method I, while P9 and P13 were also screened by method II. Method I requires more complex apparatus, but has advantages on simple detection and high sensitivity. Method II requires simpler apparatus than method I, but with more tedious detection and lower sensitivity. However, the methods established above would provide new ways for rapid detection of natural peroxynitrite-scavenging compounds in RX complex matrices.
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出版历程
  • 刊出日期:  2016-10-24

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