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和厚朴酚对小鼠背根神经节河豚毒素敏感型钠电流的影响

黄云, 康子瑶, 张广钦

黄云, 康子瑶, 张广钦. 和厚朴酚对小鼠背根神经节河豚毒素敏感型钠电流的影响[J]. 中国药科大学学报, 2016, 47(5): 609-613. DOI: 10.11665/j.issn.1000-5048.20160518
引用本文: 黄云, 康子瑶, 张广钦. 和厚朴酚对小鼠背根神经节河豚毒素敏感型钠电流的影响[J]. 中国药科大学学报, 2016, 47(5): 609-613. DOI: 10.11665/j.issn.1000-5048.20160518
HUANG Yun, KANG Ziyao, ZHANG Guangqin. Effects of honokiol on TTX-S sodium current in mouse dorsal root ganglion neurons[J]. Journal of China Pharmaceutical University, 2016, 47(5): 609-613. DOI: 10.11665/j.issn.1000-5048.20160518
Citation: HUANG Yun, KANG Ziyao, ZHANG Guangqin. Effects of honokiol on TTX-S sodium current in mouse dorsal root ganglion neurons[J]. Journal of China Pharmaceutical University, 2016, 47(5): 609-613. DOI: 10.11665/j.issn.1000-5048.20160518

和厚朴酚对小鼠背根神经节河豚毒素敏感型钠电流的影响

Effects of honokiol on TTX-S sodium current in mouse dorsal root ganglion neurons

  • 摘要: 采用全细胞膜片钳技术,观察和厚朴酚(honokiol,Hnk)对急性分离的小鼠背根神经节上河豚毒素敏感型(TTX-S)钠电流及其通道动力学的影响,探讨Hnk可能的镇痛机制及作用靶点。结果显示:和厚朴酚浓度依赖性地抑制TTX-S钠电流,30 μmol/L和厚朴酚可以使稳态激活曲线向较正电压方向偏移达10.2 mV,通道失活后恢复时间明显延长,但对稳态失活动力学特征无明显的影响。
    Abstract: To elucidate possible mechanisms and targets of honokiol(Hnk)for hte treatment of pain, the effects of Hnk on tetrodoxin-senstive(TTX-S)sodium current(INa)were studied in acutely dissociated mouse dorsal root ganglion(DRG)neurons with whole-cell patch clamp technique. Hnk inhibited TTX-S INa currents in a concentration-dependent manner. At 30 μmol/L, Hnk obviously shifted the steady state activation of TTX-S INa toward more positive potentials by 10. 2 mV and prolonged the time course of recovery of sodium current, yet with no significant difference on recovery from inactivation of TTX-S sodium channel.
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  • 刊出日期:  2016-10-24

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