• 中国中文核心期刊
  • 中国科学引文数据库核心期刊
  • 中国科技核心期刊
  • 中国高校百佳科技期刊
高级检索

基于MALDI-MS技术的新基质大黄酸在代谢组学研究中的应用

叶慧, 王赟, 王琳, 徐小为, 吴梦秋, 曹国秀, 梁艳, 王广基

叶慧, 王赟, 王琳, 徐小为, 吴梦秋, 曹国秀, 梁艳, 王广基. 基于MALDI-MS技术的新基质大黄酸在代谢组学研究中的应用[J]. 中国药科大学学报, 2016, 47(6): 727-733. DOI: 10.11665/j.issn.1000-5048.20160616
引用本文: 叶慧, 王赟, 王琳, 徐小为, 吴梦秋, 曹国秀, 梁艳, 王广基. 基于MALDI-MS技术的新基质大黄酸在代谢组学研究中的应用[J]. 中国药科大学学报, 2016, 47(6): 727-733. DOI: 10.11665/j.issn.1000-5048.20160616
shu
YE Hui, WANG Yun, WANG Lin, XU Xiaowei, WU Mengqiu, CAO Guoxiu, LIANG Yan, WANG Guangji. Rhein:a novel matrix for profiling and imaging of endogenous metabolites by matrix-assisted laser desorption/ionization-mass spectrometry[J]. Journal of China Pharmaceutical University, 2016, 47(6): 727-733. DOI: 10.11665/j.issn.1000-5048.20160616
Citation: YE Hui, WANG Yun, WANG Lin, XU Xiaowei, WU Mengqiu, CAO Guoxiu, LIANG Yan, WANG Guangji. Rhein:a novel matrix for profiling and imaging of endogenous metabolites by matrix-assisted laser desorption/ionization-mass spectrometry[J]. Journal of China Pharmaceutical University, 2016, 47(6): 727-733. DOI: 10.11665/j.issn.1000-5048.20160616
shu

基于MALDI-MS技术的新基质大黄酸在代谢组学研究中的应用

  • 中国药科大学药物代谢动力学重点实验室

基金项目: 国家自然科学基金资助项目(No.81403005,No.81273589)；江苏省自然科学基金资助项目(No.BK20140667)

Rhein:a novel matrix for profiling and imaging of endogenous metabolites by matrix-assisted laser desorption/ionization-mass spectrometry

摘要

    摘要
  • 摘要: 基质辅助激光解析电离质谱(MALDI-MS)是一项快速、高通量检测未知物质的新兴技术。然而，该技术对代谢物的检测分析往往被复杂的基质峰所干扰，限制了其在代谢组学领域的应用。研究发现，大黄酸能作为一种MALDI新基质用于阳离子模式下代谢物的检测。首先运用大黄酸分析了生理学浓度下多种强、弱碱性代谢物的标准品，结果表明与传统基质比较，大黄酸能获得基本无基质峰干扰的质谱图。进一步将大黄酸运用于生物样本的分析，选取小鼠内容物作为模型样本，显示该基质可用于复杂生物样本的代谢组分析。最后考察大黄酸的MALDI质谱成像能力，表明该基质可成功运用于小鼠肠段切片的代谢组原位成像。研究表明，大黄酸作为一种新型MALDI基质，能可靠、高效地完成基于MALDI-MS的代谢物检测及原位成像分析，对MALDI-MS技术广泛运用于代谢组学研究具有推动作用。
    Abstract: MALDI-MS is an emerging technique that enables rapid profiling of endogenous substances in a high throughput. Nevertheless, its application to metabolite analysis is often hindered by the presence of massive matrix background peaks using conventional matrices. Herein rhein was introduced as a novel matrix for MALDI-MS analysis of cation metabolites. Several strong and weak basic metabolites were measured using rhein. Compared to the previously reported ionless matrix NSA and conventional matrix CHCA, clean MALDI-MS spectra were obtained devoid of matrix signals. Subsequently, we applied this matrix to profile a biologically complex sample, mice intestinal contents. In addition, we have validated the applicability of rhein to MALDI-MS imaging, which allows for simultaneous mapping of hundreds of metabolites from single mouse ileum section. In summary, the discovery of rhein as a novel MALDI matrix enables efficient and reliable profiling and imaging of endogenous metabolites from biological samples with no matrix interferences, which will greatly promote the utility of MALDI-MS-based platform for metabolome studies.
    • HTML全文
    • 参考文献(22)
  • [1] Holmes E,Wilson ID,Nicholson JK.Metabolic phenotyping in health and disease[J].Cell,2008,134(5):714-717.
    [2] Nicholson JK,Holmes E,Kinross JM,et al.Metabolic phenotyping in clinical and surgical environments[J].Nature,2012,491(7424):384-392.
    [3] Janeková H,Hron K,Wojtowicz P,et al.Targeted metabolomic analysis of plasma samples for the diagnosis of inherited metabolic disorders[J].J Chromatogr A,2012,1226(4):11-17.
    [4] Girlanda R,Cheema AK,Kaur P,et al.Metabolomics of human intestinal transplant rejection[J].Am J Transplant,2012(12):S18-S26.
    [5] Zhang AH,Sun H,Yan GL,et al.Metabolomics study of type 2 diabetes using ultra-performance LC-ESI/quadrupole-TOF high-definition MS coupled with pattern recognition methods[J].J Physiol Biochem,2014,70(1):117-128.
    [6] Duscharla D,Bhumireddy SR,Lakshetti S,et al.Prostate cancer associated lipid signatures in serum studied by ESI-tandem mass spectrometry as potential new biomarkers[J].PLoS One,2016,11(3):e0150253.
    [7] Liu Y,Hong ZY,Tan G,et al.NMR and LC/MS-based global metabolomics to identify serum biomarkers differentiating hepatocellular carcinoma from liver cirrhosis[J].Int J Cancer,2014,135(3):658-668.
    [8] Buck A,Walch A.In situ drug and metabolite analysis in biological and clinical research by MALDI MS imaging[J].Bioanalysis,2014,6(9):1241-1253.
    [9] Ye H,Wang JX,Greer T,et al.Visualizing neurotransmitters and netabolites in the central nervous system by high resolution and high accuracy mass spectrometric imaging[J].ACS Chem Neurosci,2013,4(7):1049-1056.
    [10] Wang J,Qiu S,Chen S,et al.MALDI-TOF MS imaging of metabolites with a N-(1-naphthyl)ethylenediamine dihydrochloride matrix and its application to colorectal cancer liver metastasis[J].Anal Chem,2015,87(1):422-430.
    [11] Römpp A,Spengler B.Mass spectrometry imaging with high resolution in mass and space[J].Histochem Cell Biol,2013,139(6):759-783.
    [12] Morosi L,Spinelli P,Zucchetti M,et al.Determination of paclitaxel distribution in solid tumors by nano-particle assisted laser desorption ionization mass spectrometry imaging[J].PLoS One,2013,8(8):e72532.
    [13] Shroff R,Svatos A.Proton sponge:a novel and versatile MALDI matrix for the analysis of metabolites using mass spectrometry[J].Anal Chem,2009,81(19):7954-7959.
    [14] Strupat K,Karas M,Hillenkamp F.2,5-Dihydroxybenzoic acid:a new matrix for laser desorption-ionization mass spectrometry[J].Int J Mass Spectrom,1991,111:89-102.
    [15] Shariatgorji M,Källback P,Gustavsson L,et al.Controlled-pH tissue cleanup protocol for signal enhancement of small molecule drugs analyzed by MALDI-MS imaging[J].Anal Chem,2012,84(10):4603-4607.
    [16] Fukuyama Y, Nakajima C, Furuichi K, et al. Alkylated trihydroxyacetophenone as a MALDI matrix for hydrophobic peptides[J].Anal Chem,2013,85(20):9444-9448.
    [17] Shroff R, Rulíšek L, Doubsk J, et al. Acid-base-driven matrix-assisted mass spectrometry for targeted metabolomics[J].Proc Natl Acad Sci U S A,2009,106(25):10092-10096.
    [18] Wang Z,Klipfell E,Bennett BJ,et al.Gut flora metabolism of phosphatidylcholine promotes cardiovascular disease[J].Nature,2011,472(7341):57-63.
    [19] Tang WH,Hazen SL.The contributory role of gut microbiota in cardiovascular disease[J].J Clin Invest,2014,124(10):4204-4211.
    [20] Zhu W,Gregory JC,Org E,et al.Gut microbial metabolite TMAO enhances platelet hyperreactivity and thrombosis risk[J].Cell,2016,165(1):111-124.
    [21] Tian Y,Zhang L,Wang Y,et al.Age-related topographical metabolic signatures for the rat gastrointestinal contents[J].J Proteome Res,2012,11(2):1397-1411.
    [22] Bergen WG,Wu G.Intestinal nitrogen recycling and utilization in health and disease[J].J Nutr,2009,139(5):821-825.
    • 相关文章
    • 施引文献
    • 资源附件(0)
计量
  • 文章访问数:  931
  • HTML全文浏览量:  0
  • PDF下载量:  1454
  • 被引次数: 0
出版历程
  • 刊出日期:  2016-12-24

目录

摘要
HTML全文
    参考文献(22)
    相关文章
    施引文献
    资源附件(0)

    /

    返回文章
    返回

    版权所有：《中国药科大学学报》编辑部苏ICP备05007142号

    地址：江苏省南京市鼓楼区童家巷24号（210009）电话: 025-83271566E-mail: xuebao@cpu.edu.cn

    本系统由 北京仁和汇智信息技术有限公司 开发  百度统计

    访问量：400216

    x 关闭 永久关闭