肿瘤相关巨噬细胞共培养体系中人参及其主要成分对肺癌A549细胞的作用
Effect of Ginseng, Ginseng polysaccharides and Ginsenoside on the human lung cancer cells A549 in co-culture system of TAMs with A549 cells
-
摘要: 通过人参水提液(water extract of Ginseng，WEG)、人参多糖(Ginseng polysaccharides，GPS)、人参皂苷(Ginseng total saponins，GTS)对肿瘤相关巨噬细胞(tumor-associated macrophages，TAMs)与肿瘤细胞共培养体系中肺癌A549细胞增殖、迁移和细胞骨架的影响研究，探讨人参抗肿瘤的作用机制。建立THP-1诱导的TAMs体外模型，采用上清液共培养体系，以肺癌A549细胞为研究对象，并采用MTT法观察不同浓度的WEG、GPS、GTS作用于共培养体系中对肺癌A549细胞增殖的影响及量效关系；通过实时细胞分析技术(RTCA)检测WEG、GPS、GTS对共培养体系中肺癌A549细胞迁移能力的影响，免疫荧光高内涵细胞分析系统(HCS)检测细胞骨架蛋白F-actin的表达。结果显示:WEG能明显抑制共培养体系中A549细胞的增殖、迁移能力和减少骨架面积及微丝数量(P<；0.01)；GPS能明显抑制共培养体系中A549细胞迁移能力、骨架面积和微丝数量(P<；0.01)，但对A549细胞的增殖作用无明显影响；GTS能明显抑制共培养体系中A549细胞的增殖(P<；0.01)，而对A549细胞的迁移能力、骨架面积和微丝无影响。通过对WEG、GPS、GTS的研究对比，发现人参及两种主要成分对TAMs共培养体系中肺癌A549细胞都有影响，但作用存在差异，提示人参抗肿瘤作用与其多成分对肿瘤免疫微环境调控相关，且不同成分间可能存在协同关系。Abstract: This study was aimed to explore the anti-tumor functions of Ginseng on the human lung cancer cells A549, through investigating the effects of water extract of Ginseng(WEG), Ginseng polysaccharides(GPS)and Ginseng total saponins(GTS)on cell proliferation, cell migration and cytoskeleton of A549 cells. Using A549 cells as target cells, the TAMs model in vitro was established from THP-1. MTT method was used to observe the effects of different concentrations of WEG, GPS, GTS on A549 cells in co-culture system of TAMs with A549 cells. The cell migration of A549 cells was assayed by real-time cell analysis(RTCA). The cytoskeleton of A549 cells was detected by high content screening(HCS). It showed that in the co-culture system, WEG inhibited the proliferation, migration, and the area of cytoskeleton on A549 cells significantly(P< 0. 01). GPS inhibited A549 cell migration and the area of cytoskeleton(P < 0. 01)in co-culture system, but it showed no significant effect on the proliferation of A549 cells. Moreover, GTS can significant inhibit the proliferation of A549 cells(P< 0. 01), and the effect on the cell migration and the area of cytoskeleton was in conspicuous in the co-culture system. It was found that the two main components of ginseng showed different functions by the comparison of WEG, GPS, GTS in TAMs co-culture system. Results indicate that the antitumor effects of ginseng may be due to its multi-component regulation in the tumor microenvironment.
-
Keywords:
- tumor-associated macrophages /
- co-cuture /
- Ginseng /
- Ginseng polysaccharides /
- Ginseng total saponins /
- A549 cells
-
-
[1] Shen CM,Li QL,Zhang HY,et al.Analysis of Chinese medicine prescriptions for cancer[J].Chin Archives Tradit Chin Med(中华中医药学刊),2013,31(11):2475-2477. [2] Jin YH,Yook J,Lee YH,et al.Caspase3-mediated cleavage of p21WAF1/CIP1 associated with the cylin A-cyclin-dependent kinase 2 complex is a prerequisite for apoptosis in SK-HEP-1 cells[J].J Biol Chem,2000,275(39):30256-30263. [3] Mochizuki M,Yoo YC,Matsuzawa K,et al.Inhibitory effect of tumor metastasis in mice by saponins,ginsenoside-Rb2,20(R)-and 20(S)-ginsenoside-Rg3,of renginseng[J].Biol Pharm Bull,1995,18(9):1197-1202. [4] Kim HE,Oh JH,Lee SK,et al.Ginsenoside RH-2 induces apoptotic cell death in rat C6 glioma via a reactive oxygen-and caspase-dependent but Bcl-X(L)-independent pathway[J].Life Sci,1999,65(3):PL33-40. [5] Wang P,Du X,Xiong M,et al.Ginsenoside Rd attenuates breast cancer metastasis implicating derepressing microRNA-18a-regulated Smad2 expression [J].Scientific Reports,2016,6:33709. [6] Solinas G, Germano G, Mantovani A, et al. Tumor-associated macrophages(TAMS)as major players of the cancer-related inflammation[J].J Leukoc Biol,2009,86(5):1065-1073. [7] Jiang YC,Bi L,Gao J,et al.Research of immune modulation of Cinseng water extracts on TAMs and its regulation effects on cell proliferation of A549[J].Chin Tradit Patent Med(中成药),2016,38(8):1689-1695. [8] Condeelis J,Pollard JW.Macrophages:obligate partners for tumor cell migration,invasion,and metastasis [J].Cell,2006,124:263-266. [9] Wallerius M,Wallmann T,Bartish M,et al.Guidance molecule SEMA3A restricts tumor growth by differentially regulating the proliferation of tumor-associated macrophages [J].Cancer Res,2016,76:3166-3178.
计量
- 文章访问数:
- HTML全文浏览量: 0
- PDF下载量:
下载:
