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7-O-丁二酰大环内酯菌素A对人肺癌细胞H460的抑制作用及其机制

杨培树, 郝文立

杨培树, 郝文立. 7-O-丁二酰大环内酯菌素A对人肺癌细胞H460的抑制作用及其机制[J]. 中国药科大学学报, 2017, 48(1): 82-88. DOI: 10.11665/j.issn.1000-5048.20170113
引用本文: 杨培树, 郝文立. 7-O-丁二酰大环内酯菌素A对人肺癌细胞H460的抑制作用及其机制[J]. 中国药科大学学报, 2017, 48(1): 82-88. DOI: 10.11665/j.issn.1000-5048.20170113
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YANG Peishu, HAO Wenli. Inhibitory effect and its mechanism of 7-O-succinyl macrolactin A against cell proliferation, invasion and migration in human lung cancer H460 cells[J]. Journal of China Pharmaceutical University, 2017, 48(1): 82-88. DOI: 10.11665/j.issn.1000-5048.20170113
Citation: YANG Peishu, HAO Wenli. Inhibitory effect and its mechanism of 7-O-succinyl macrolactin A against cell proliferation, invasion and migration in human lung cancer H460 cells[J]. Journal of China Pharmaceutical University, 2017, 48(1): 82-88. DOI: 10.11665/j.issn.1000-5048.20170113
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7-O-丁二酰大环内酯菌素A对人肺癌细胞H460的抑制作用及其机制

  • 1.

    天津市中医药研究院附属医院药剂科

  • 2.

    天津市中医药研究院附属医院疮疡科

Inhibitory effect and its mechanism of 7-O-succinyl macrolactin A against cell proliferation, invasion and migration in human lung cancer H460 cells

摘要

    摘要
  • 摘要: 探讨7-O-丁二酰大环内酯菌素A对人肺癌细胞侵袭转移的抑制作用及其机制。7-O-丁二酰大环内酯菌素A处理人肺癌细胞H460后，采用MTS、细胞黏附、Transwell和划痕实验观察7-O-丁二酰大环内酯菌素A对细胞生长、体外黏附能力及侵袭转移的影响，流式细胞术检测细胞周期和凋亡的变化，RT-PCR和Western blot检测7-O-丁二酰大环内酯菌素A对β-catenin、c-Myc、Cyclin D1、vimentin、N-cadherin、CD44、integrin β1、bcl-2、Survivin和MMP-2/9的mRNA和蛋白表达的影响以及AKT和mTOR的磷酸化的变化。结果表明:7-O-丁二酰大环内酯菌素A显著抑制H460细胞的体外增殖和体外黏附，诱导细胞发生凋亡，且抑制细胞体外迁移和侵袭能力。Western blot和Real-time PCR结果显示，7-O-丁二酰大环内酯菌素A下调细胞中Bcl-2、Survivin、β-catenin、c-Myc、Cyclin D1、vimentin、N-cadherin、CD44、integrin β1和MMP-2/9的表达，以及AKT和mTOR的磷酸化。7-O-丁二酰大环内酯菌素A可抑制人肺癌细胞H460的体外生长，并抑制其体外黏附和侵袭转移能力。
    Abstract: This study aimed at investigating the effects and mechanisms of 7-O-succinyl macrolactin A in inhibiting human lung cancer. After treatment of human lung cancer cell lines H460 with 7-O-succinyl macrolactin A, MTS assay was employed to determine cell proliferation; crystal violet staining was used to detect cell adhesion of H460; transwell chamber assay and wound healing assay were performed to evaluate cell invasion and migration; and flow cytometry assay was adopted to evaluate cell cycle. Western blotting and real-time PCR were also employed to determine the expression of β-catenin, c-Myc, Cyclin D1, vimentin, N-cadherin, CD44, integrin β1, Bcl-2, Survivin and MMP-2/9. The phosphorylation of AKT and mTOR was determined as well. In vitro proliferation of H460 was inhibited significantly by 7-O-succinyl macrolactin A. Cell adhesion, invasion and migration abilities were also attenuated. Western blot and real-time PCR showed that the expressions of β-catenin, c-Myc, cyclin D1, vimentin, N-cadherin, CD44, integrin β1, Bcl-2, Survivin and MMP-2/9 were down-regulated by 7-O-succinyl macrolactin A. It was also found that phosphorylation of AKT and mTOR was inhibited by 7-O-succinyl macrolactin A. 7-O-succinyl macrolactin A can inhibit the in vitro growth and invasion of human lung cancer cell lines H460.
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  • 刊出日期:  2017-02-24

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