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蜂胶化学成分的LC-IT-TOF-MS鉴别

姚静, 孙建国, 吴亮, 汪豪, 王广基, 叶文才

姚静, 孙建国, 吴亮, 汪豪, 王广基, 叶文才. 蜂胶化学成分的LC-IT-TOF-MS鉴别[J]. 中国药科大学学报, 2017, 48(2): 178-183. DOI: 10.11665/j.issn.1000-5048.20170208
引用本文: 姚静, 孙建国, 吴亮, 汪豪, 王广基, 叶文才. 蜂胶化学成分的LC-IT-TOF-MS鉴别[J]. 中国药科大学学报, 2017, 48(2): 178-183. DOI: 10.11665/j.issn.1000-5048.20170208
YAO Jing, SUN Jianguo, WU Liang, WANG Hao, WANG Guangji, YE Wencai. Identification of major constituents from propolis by LC-IT-TOF-MS[J]. Journal of China Pharmaceutical University, 2017, 48(2): 178-183. DOI: 10.11665/j.issn.1000-5048.20170208
Citation: YAO Jing, SUN Jianguo, WU Liang, WANG Hao, WANG Guangji, YE Wencai. Identification of major constituents from propolis by LC-IT-TOF-MS[J]. Journal of China Pharmaceutical University, 2017, 48(2): 178-183. DOI: 10.11665/j.issn.1000-5048.20170208

蜂胶化学成分的LC-IT-TOF-MS鉴别

基金项目: 国家自然科学基金资助项目(No.81573309)

Identification of major constituents from propolis by LC-IT-TOF-MS

  • 摘要: 建立液相色谱串联离子阱飞行时间质谱法(LC-IT-TOF-MS)鉴定中国蜂胶的化学成分。采用Shim-pack VP-ODS色谱柱(2.0 mm×150 mm,5 μm),流动相为乙腈-0.2%甲酸水溶液,梯度洗脱,流速0.3 mL/min,采用ESI-IT-TOF检测,负离子模式扫描。根据色谱峰精确相对分子质量、碎片离子信息、及液相色谱保留时间比较鉴别化合物。采用LC-IT-TOF-MS法鉴定了中国蜂胶(山东泰安)中31个成分,包括25个黄酮类化合物和6个酚酸类化合物。结果表明,采用LC-IT-TOF-MS方法可以快速、准确地对蜂胶中各成分进行有效的定性分析。
    Abstract: A qualitative analytical method of liquid chromatography coupled with ion trap time-of-flight mass spectrometry(LC-IT-TOF/MS)was developed for identification of major constituents in propolis from China(Shandong). The LC-IT-TOF-MS was performed on a Shim-pack VP-ODS column(2. 0 mm×150 mm, 5 μm)with the mobile phase consisting of acetonitrile and water containing 0. 2% formic acid in gradient mode, and the flow rate was set at 0. 3 mL/min. Negative ion mode was used for IT-TOF-MS. According to the accurate molecular weight, MS fragment pathway, comparison with the retention time of reference compounds, total 31 compounds, including twenty-five phenolic acids and six flavonoids were identified. The LC-IT-TOF-MS qualitative analysis method can be used for analyzing major components of propolis quickly and accurately.
  • [1] Gardana C,Scaglianti M,Pietta P,et al.Analysis of the polyphenolic fraction of propolis from different sources by liquid chromatography-tandem mass spectrometry[J].J Pharm Biomed Anal,2007,45(3):390-399.
    [2] Kumazawa S,Hamasaka T,Nakayama T.Antioxidant activity of propolis of various geographic origins[J].Food Chem,2004,84(3):329-339.
    [3] Wang K,Zhang CP,Hu F.Research status of propolis in 2014[J].J Bee(蜜蜂杂志),2015,35(3):1-6.
    [4] Zhang YZ,Xu F,Zhang JY,et al.Investigations of the fragmentation behavior of 11 isoflavones with ESI-IT-TOF-MSn[J].J China Pharm Sci(中国药学),2014,23(9):631-641.
    [5] Liang Y,Hao HP,et al.Qualitative and quantitative determination of complicated herbal components by liquid chromatography hybrid ion trap time-of-flight mass spectrometry and a relative exposure approach to herbal pharmacokinetics independent of standards[J].J Chromatogr A,2010,1217(30):4971-4979.
    [6] Zheng CN,Hao HP,Wang X,et al.Diagnostic fragment-ion-based extension strategy for rapid screening and identification of serial components of homologous families contained in traditional Chinese medicine prescription using high-resolution LC-ESI-IT-TOF/MS: Shengmai injectionas an example[J].J Mass Spectrom,2009,44(2):230-244.
    [7] Geng CA,Chen XL,Zhou NJ,et al.LC-MS Guided isolation of(± )-Swe-riledugenin A,a pair of enantiomeric lactones,from Swertia leducii[J].Org Lett,2014,16(26):370-373.
    [8] Pellati F,Orlandini G,Pinetti D,et al.HPLC-DAD and HPLC-ESI-MS/MS methods for metabolite profiling of propolis extracts[J].J Pharm Biomed Anal,2011,55(5):934-948.
    [9] Falcão SI,Vale N,Gomes P,et al.Phenolic profiling of Portuguese propolis by LC-MS spectrometry:uncommon propolis rich in flavonoid glycosides[J].Phytochem Anal,2013,24(4):309-318.
    [10] Ding JH,Wang XX,Zhang H,et al.Extrative electrospray ionization tandem mass spectrometry of apigenin[J].Chem J Chin Univ(高等学校化学学报),2011,32(8):1714-1719.
    [11] Liu RX,Ye M,Guo HZ,et al.Liquid chromatography/electrospray ionization mass spectrometry for the characterization of twenty-three flavonoids in the extract of Dalbergia odorifera[J].Rapid Commun Mass Spectrom,2005,19(11):1557-1565.
    [12] Righi AA,Negri G,Salatino A.Comparative chemistry of propolis from eight Brazilian Localities[J].Evid Based Complement Alternat Med,2013,2013(878):267878.
    [13] Wang YX,Hao HP,Wang GJ.Application of Q-TOF and IT-TOF mass spectrometry technology in identifying the natural products and their biological metabolites[J].Chin J Nat Med(中国天然药物),2009,7(5):394-400.
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出版历程
  • 刊出日期:  2017-04-24

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