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原位生成离子液体法微萃取-超高效液相色谱法测定万古霉素血药浓度

许德金, 袁文博, 吕娟

许德金, 袁文博, 吕娟. 原位生成离子液体法微萃取-超高效液相色谱法测定万古霉素血药浓度[J]. 中国药科大学学报, 2017, 48(2): 196-200. DOI: 10.11665/j.issn.1000-5048.20170210
引用本文: 许德金, 袁文博, 吕娟. 原位生成离子液体法微萃取-超高效液相色谱法测定万古霉素血药浓度[J]. 中国药科大学学报, 2017, 48(2): 196-200. DOI: 10.11665/j.issn.1000-5048.20170210
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XU Dejin, YUAN Wenbo, LYU Juan. Determination of vancomycin in human serum by in situ formed ionic liquids microextraction-ultra performance liquid chromatography(ISFILM-UPLC)methods[J]. Journal of China Pharmaceutical University, 2017, 48(2): 196-200. DOI: 10.11665/j.issn.1000-5048.20170210
Citation: XU Dejin, YUAN Wenbo, LYU Juan. Determination of vancomycin in human serum by in situ formed ionic liquids microextraction-ultra performance liquid chromatography(ISFILM-UPLC)methods[J]. Journal of China Pharmaceutical University, 2017, 48(2): 196-200. DOI: 10.11665/j.issn.1000-5048.20170210
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原位生成离子液体法微萃取-超高效液相色谱法测定万古霉素血药浓度

  • 无锡市第四人民医院药学部

基金项目: 无锡市医院管理中心医学科研药学项目资助(No.YGZXY1312)

Determination of vancomycin in human serum by in situ formed ionic liquids microextraction-ultra performance liquid chromatography(ISFILM-UPLC)methods

摘要

    摘要
  • 摘要: 建立原位生成离子液体法微萃取-超高效液相色谱联用技术测定万古霉素血药浓度的方法。以溴化1-己基-3-甲基咪唑(［C6MIM］［Br］)和六氟磷酸钠为提取剂，甲硝唑为内标物，样品经涡旋振荡、离心等处理后，以甲醇复溶下层沉淀作为供试液。供试液测定采用BEH C18柱，甲醇-0.05 mol/L磷酸二氢钾为流动相，流速为0.2 mL/min，进样量为5 μL，柱温为18 ℃。方法的准确度、精密度、专属性等完全符合定量测定要求。本法测定准确，与临床常用的免疫分析法结果比较，重复性良好，具有良好的临床应用前景。
    Abstract: A method based on in situ formed ionic liquids microextraction-ultra performance liquid chromatography(ISFILM-UPLC)was established for the determination of vancomycin in human serum. The samples were pretreated by vortex and centrifugation. ［C6MIM］［Br］ and NaPF6 were applied as the extracting agent, and Metronidazole as the internal standard. Methanol was applied to redissolve the precipitate. The separation was carried out on a BEH C18 column, and the mobile phase consisted of methanol and 0. 05 mol/L monopotassium phosphate solution. The flow rate was 0. 2 mL/min, the injection volume was 5 μL, and the column temperature was 18 °C. The key parameters of this method such as the specificity, accuracy and precision were validated. This method showed good correlation with the immunoassay. It shows great potential for the application in clinical practice.
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    • 参考文献(17)
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  • 刊出日期:  2017-04-24

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