蒿甲醚联合依托泊苷对小细胞肺癌细胞株H446增殖及侵袭的抑制作用
Combination of artemether with etoposide inhibits cell proliferation and invasion in small cell lung cancer cell line H446
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摘要: 探讨蒿甲醚与依托泊苷联用对小细胞肺癌细胞H446增殖及侵袭能力的抑制作用。采用MTT法检测蒿甲醚、依托泊苷单用及联合作用对H446细胞增殖的影响；采用Annexin V/PI荧光双染法检测蒿甲醚、依托泊苷单用及联合作用对H446细胞凋亡的影响；采用PI单染法检测蒿甲醚、依托泊苷单用及联合作用对H446细胞周期分布的影响。采用基质胶侵袭实验检测蒿甲醚、依托泊苷单用及联合作用对H446侵袭能力的影响。结果表明，蒿甲醚、依托泊苷单用以及联合使用均能有效抑制H446的增殖，且呈剂量依赖性，两药联用具有协同作用。蒿甲醚单用不能明显促进细胞凋亡，但与依托泊苷(300 nmol/L)联用72 h可以比较明显促进H446凋亡，具有统计学意义(P<；0.05)。蒿甲醚单用对H446的细胞周期没有显著影响，联合作用48 h对细胞周期G2期阻滞主要是依托泊苷的作用。单用蒿甲醚、依托泊苷以及二者联合使用都能显著抑制H446的侵袭能力。Abstract: This study was to investigate the inhibitory effects of artemether in combination with etoposide on the proliferation and invasion ability of human small cell lung cancer cell line H446. H446 cells were treated with different concentrations of artemether or etoposide alone or their combination. The inhibitory effects on proliferation were detected by MTT assay, while cell cycle and apoptosis of H446 cells in each group were analyzed by flow cytometry using PI and Annexin V/PI-staining, respectively. The invasion capability of H446 cells in different groups was tested with matrigel-coated transwell. The results implicated that artemether or etoposide or their combination does inhibit proliferation of H446 cells dose-dependently. Artemether alone had little effect on the apoptosis of H446 cells while its combination with etoposide resulted in significantly apoptosis of H446 cells comparing with other groups(P< 0. 05). Etoposide blocked H446 progression markedly by arresting cell cycle in G2 phase with percentage of cells in G1 phase decreasing significantly while artemether alone or in combination with etoposide had little synergetic effect on cell cycle. Artemether or etoposite alone or their combination could dramatically inhibit the invasion ability of H446 cells.
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Keywords:
- artemether /
- etoposide /
- small cell lung cancer /
- drug combination /
- proliferation /
- invasion
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