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虫草多糖CPS-A对血管紧张素Ⅱ诱导肝L02细胞损伤的保护作用

查正其, 陈秋利, 王玉峰, 祝慧, 王莹, 尹鸿萍, 乐龙

查正其, 陈秋利, 王玉峰, 祝慧, 王莹, 尹鸿萍, 乐龙. 虫草多糖CPS-A对血管紧张素Ⅱ诱导肝L02细胞损伤的保护作用[J]. 中国药科大学学报, 2017, 48(4): 490-495. DOI: 10.11665/j.issn.1000-5048.20170415
引用本文: 查正其, 陈秋利, 王玉峰, 祝慧, 王莹, 尹鸿萍, 乐龙. 虫草多糖CPS-A对血管紧张素Ⅱ诱导肝L02细胞损伤的保护作用[J]. 中国药科大学学报, 2017, 48(4): 490-495. DOI: 10.11665/j.issn.1000-5048.20170415
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ZHA Zhengqi, CHEN Qiuli, WANG Yufeng, ZHU Hui, WANG Ying, YIN Hongping, YUE Long. Protective effects of Cordyceps sinensis polysaccharide CPS-A on angiotensin II-induced injury of liver L02 cells[J]. Journal of China Pharmaceutical University, 2017, 48(4): 490-495. DOI: 10.11665/j.issn.1000-5048.20170415
Citation: ZHA Zhengqi, CHEN Qiuli, WANG Yufeng, ZHU Hui, WANG Ying, YIN Hongping, YUE Long. Protective effects of Cordyceps sinensis polysaccharide CPS-A on angiotensin II-induced injury of liver L02 cells[J]. Journal of China Pharmaceutical University, 2017, 48(4): 490-495. DOI: 10.11665/j.issn.1000-5048.20170415
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虫草多糖CPS-A对血管紧张素Ⅱ诱导肝L02细胞损伤的保护作用

  • 1.

    中国药科大学生命科学与技术学院

  • 2.

    南京中医药大学药学院

  • 3.

    威海人生集团股份有限公司

  • 4.

    中国药科大学工学院

基金项目: 江苏省科技攻关项目资助(No.S152001)

Protective effects of Cordyceps sinensis polysaccharide CPS-A on angiotensin II-induced injury of liver L02 cells

摘要

    摘要
  • 摘要: 探讨虫草多糖CPS-A对Ang Ⅱ诱导的人肝L02细胞损伤的保护作用。MTT法考察Ang Ⅱ、CPS-A对L02细胞增殖的影响，Ang Ⅱ刺激L02细胞后，考察CPS-A对L02细胞的损伤保护作用；PCR、Real-Time PCR和Western blot法检测IL-6、IL-1β、AT1R、AT2R、NF-κB p65、TNFα等因子mRNA和蛋白质的表达水平。结果表明:Ang Ⅱ、CPS-A有效抑制L02细胞增殖的浓度分别为1×10-5 mol/L和200 μg/mL，PCR、Real-Time PCR和Western blot结果显示，CPS-A可以显著下调IL-6、IL-1β、TNF-α、NF-κB和AT1R的表达。CPS-A对Ang Ⅱ诱导的L02细胞损伤具有很好的保护作用。
    Abstract: This study aimed at the molecular mechanism of Cordyceps sinensis polysaccharide-A(CPS-A)on angiotensin(Ang II)-induced injury of L02 cells. The effect of AngII and CPS-A on the proliferation of L02 cells was analyzed by MTT assay. PCR, Real-Time PCR and Western blot were also employed to determine the expression of IL- 1β, AT1R, AT2R, NF-κB p65, TNFα and other inflammatory factors at mRNA and protein levels. The results showed that AngⅡ and CPS-A could inhibit the proliferation of L02 cells by 1×10-5 mol/L and 200 μg/mL, respectively. PCR, Real-Time PCR and Western blot showed that CPS-A could significantly down-regulate IL-1β, TNF-α, NF-κB and AT1R. CPS-A has a good protective effect on AngII-induced L02 cell injury.
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    • 参考文献(15)
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  • 刊出日期:  2017-08-24

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