泽泻提取物对STZ糖尿病大鼠的干预作用

操兰洁; 诸夔妞; 蒋翠花; 乔丹·甘恩; 车镇涛; 张健; 殷志琦

doi:10.11665/j.issn.1000-5048.20170516

泽泻提取物对STZ糖尿病大鼠的干预作用

1.
中国药科大学天然药物化学教研室&天然药物活性组分与药效国家重点实验室
2.
江苏省中医药研究院转化医学实验室
3.
美国伊利诺伊大学芝加哥校区，药学院，药物化学和生药学系，世界卫生组织传统医学中心

基金项目: 江苏省自然科学基金资助项目(No.BK20161460)；江苏高校优势学科建设工程资助项目

计量
- 文章访问数: 881
- HTML全文浏览量: 0
- PDF下载量: 1297
出版历程
- 刊出日期: 2017-10-24

Intervention effects of the extract of Alismatis Rhizoma on streptozotocin-induced type 2 diabetic rats

摘要

摘要: 为评价泽泻乙酸乙酯部位对2型糖尿病的降血糖作用及其机制，采用高脂高糖饲料喂养大鼠4周后再腹腔注射小剂量链尿佐菌素(STZ)的方法，建立2型糖尿病大鼠模型。将糖尿病大鼠随机分为模型组、泽泻乙酸乙酯部位组(AREEA，20，50，100 mg/kg)、二甲双胍组(100 mg/kg)。连续给药4周后进行葡萄糖耐量和胰岛素耐量实验。于末次给药后禁食12 h，采血测定大鼠空腹血糖(GLU)、糖化血红蛋白(HbA1c)、胰岛素(Ins)以及胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)、丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)含量，脂肪组织中胰岛素信号通路IRS-1和Akt的磷酸化水平及胰脏的病理变化。结果显示，给药组大鼠的空腹血糖有明显降低(P<；0.05)；100 mg/kg组显著降低血清GLU、HbA1c、TC、TG、MDA、TNF-α、IL-6(P<；0.05)；明显升高SOD、GSH-Px(P<；0.05)。这些结果提示泽泻乙酸乙酯部位可有效调节体内糖、脂代谢，对2型糖尿病有一定治疗作用。
- 泽泻 /
- 乙酸乙酯部位 /
- 链脲佐菌素 /
- 2型糖尿病 /
- 降血糖
Abstract: To investigate the hypoglycemic effects of ethyl acetate extract of Alismatis Rhizoma(AREEA)on type 2 diabetes mellitus, the model of type 2 diabetic rats was induced by high-fat diet feeding for 4 weeks and then intraperitoneal injection of a low dose of streptozotoin(STZ). Rats without the above-mentioned treatment were selected as the normal control group. The diabetic rats were randomly divided into 5 groups: the model control group, low doses(20 mg/kg), medium doses(50 mg/kg), high doses(100 mg/kg)of AREEA groups and positive control-metformin group(100 mg/kg). After four weeks, oral glucose tolerance test(OGTT)and insulin tolerance test(ITT)were performed, respectively. 12 hours after the last administration, the levels of serum glucose, glycated hemoglobin(HbA1c), insulin(Ins), total cholesterol(TC), triglyceride(TG), high density lipoprotein(HDL-C), low density lipoprotein(LDL-C), superoxide dismutase(SOD), malondialdehyde(MDA), glutathione(GSH-Px), tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), insulin-mediated tyrosine of IRS-1 and Akt phosphorylation in adipose tissue were determined. In addition, the pathological changes of pancreas were examined. After administration for 4 weeks, all doses of AREEA significantly reduced the fasting blood glucose of type 2 diabetic rats(P< 0. 05). In the high doses group of AREEA, the levels of GLU, HbA1c, TC, TG, MDA, TNF-α and IL-6 in serum were decreased significantly, and the levels of SOD and GSH-Px were increased(P< 0. 05). These results suggest that AREEA has the therapeutic effect on type 2 diabetes-related symptoms by metabolic regulation of glucose and lipids.
- Alismatis Rhizoma /
- ethyl acetate extract /
- streptozotoin /
- type 2 diabetes mellitus /
- hypoglycemic effect

HTML全文

参考文献(26)

[1]	Stumvoll M,Goldstein BJ,van Haeften TW.Type 2 diabetes:principles of pathogenesis and therapy[J]. Lancet,2005,365(9467):1333-1346.
[2]	Aslan M,Orhan D,Orhan N,et al. In vivo antidiabetic and antioxidant potential of Helichrysum plicatum ssp.plicatum capitulums in streptozotocin-induced-diabetic rats[J]. J Ethnopharmacol,2007,109(1):54-59.
[3]	Whiting D,Guariguata L,Weil C,et al.IDF diabetes atlas:global estimates of the prevalence of diabetes for 2011 and 2030[J]. Diabetes Res Clin Pract,2011,94(3):311-321.
[4]	Heller A,Feldman B.Electrochemistry in diabetes management[J]. Acc Chem Res,2010,43(7):963-973.
[5]	Evans JM,Ogston SA,Emslie-Smith A,et al.Risk of mortality and adverse cardiovascular outcomes in type 2 diabetes:a comparison of patients treated with sulfonylureas and metformin[J]. Diabetologia,2006,49(5):930-936.
[6]	Ahmadian M,Suh J M,Hah N,et al.PPARgamma signaling and metabolism:the good,the bad and the future[J]. Nat Med,2013,19(5):557-566.
[7]	Deng Y,He K,Ye X,et al.Saponin rich fractions from Polygonatum odoratum(Mill.)Druce with more potential hypoglycemic effects[J]. J Ethnopharmacol,2012,141(1):228-233.
[8]	Jiang S,Ren D,Li J,et al.Effects of compound K on hyperglycemia and insulin resistance in rats with type 2 diabetes mellitus[J]. Fitoterapia,2014,95:58-64.
[9]	Xie W,Zhao Y,Du L.Emerging approaches of traditional Chinese medicine formulas for the treatment of hyperlipidemia[J]. J Ethnopharmacol,2012,140(2):345-367.
[10]	Yoshikawa M,Hatakeyama S,Tankeyama N,et al.Crude drugs from aquatic plants.I.On the constituents of Alismatis Rhizoma.(1).Absolute stereostructures of alisols E 23-acetate,F,and G,three new protostane-type triterpenes from Chinese Alismatis Rhizoma[J]. Chem Pharm Bull,2012,23(7):1948-1954.
[11]	Yang XB,Huang ZM,Cao WB,et al.Therapeutic and protective effects of water-ethanolic extract from Rhizoma Alismatis on streptozotocin-induced diabetic mice[J]. Pharm J Chin PLA(解放军药学学报),2012,18(6):336-338.
[12]	Xu W,Luo FR,Zhao WL,et al.Chemical constituents from Alisma orientalis extracts with hypoglycemic effect[J]. China Tradit Herb Drugs(中草药),2014,45(22):3238-3245.
[13]	Zhong WC,Zhu GF,Li WL,et al.The etiology and pathogenesis of diabetes[J]. Lishizhen Med Mat Med Res(时珍国医国药),2012,23(7):1770-1772.
[14]	Ma H.Clinical observation of the treatment of diabetes by TCM differentiation[J]. Prac Clin J Integr Tradi ChinWest Med(实用中西医结合临床),2014,14(1):57-58.
[15]	Zhu KN,Jiang CH,Tian YS,et al.Two triterpeniods from Cyclocarya paliurus(Batal)Iljinsk(Juglandaceae)promote glucose uptake in 3T3-L1 adipocytes:the relationship to AMPK activation[J]. Phytomedicine,2015,22(9):837-846.
[16]	Jung JY,Lim Y,Moon MS,et al.Onion peel extracts ameliorate hyperglycemia and insulin resistance in high fat diet/streptozotocin-induced diabetic rats[J]. Nutr Metab,2011, 8(1):18.
[17]	Ren C,Zhang Y,Cui W,et al.A polysaccharide extract of mulberry leaf ameliorates hepatic glucose metabolism and insulin signaling in rats with type 2 diabetes induced by high fat-diet and streptozotocin[J]. Int J Biol Macro Mol,2015,72:951-959.
[18]	Callejas D,Mann CJ,Ayuso E,et al.Treatment of diabetes and long-term survival following insulin and glucokinase gene therapy[J]. Diabetes,2013,62(5):1718-1729.
[19]	Han JW,Zhan XR,Li XY,et al.Impaired PI3K/Akt signal pathway and hepatocellular injury in high-fat fed rats[J]. World J Gastroenterol,2010,16(48):6111-6118.
[20]	Bjornholm M,Zierath JR.Insulin signal transduction in human skeletal muscle:identifying the defects in type II diabetes[J]. Biochem Soc Trans,2005,33(2):354-357.
[21]	Jackson S,Bagstaff SM,Lynn S,et al.Decreased insulin responsiveness of glucose uptake in cultured human skeletal muscle cells from insulin-resistant nondiabetic relatives of type 2 diabetic families[J]. Diabetes,2000,49(7):1169-1177.
[22]	Lu J, Bi Y, Wang T, Wang W, et al. The relationship between insulin-sensitive obesity and cardiovascular diseases in a Chinese population:results of the reaction study[J]. Int J Cardiol, 2014, 172(2):388-394.
[23]	Tian JL, Zhou SL, Wang XH, et al. Improvable effect of lipid-lowering treatment on early insulin secretion in T2DM patients with dyslipidemia[J]. Chin J Diabetes(中国糖尿病杂志),2013,21(4):312-314.
[24]	Jennings PE,Mclaren M,Scott NA,et al.The relationship of oxidative stress to thrombotic tendency in type 1 diabetic patients with retinopathy[J]. Diabetic Med,1991,8(9):860-865.
[25]	Djordjevic A,Spasic S,Jovanovic-Galovic A,et al.Oxidative stress in diabetic pregnancy:SOD,CAT and GSH-Px activity and lipid peroxidation products[J]. J Matern Fetal Neonatal Med,2004,16(6):367-372.
[26]	Esposito K,Nappo F,Marfella R,et al.Inflammatory cytokine concentrations are acutely increased by hyperglycemia in humans:role of oxidative stress[J]. Circulation,2002,106(16):2067-2072.

施引文献

资源附件(0)

计量

文章访问数: 881
HTML全文浏览量: 0
PDF下载量: 1297
被引次数: 0

泽泻提取物对STZ糖尿病大鼠的干预作用

计量

出版历程

Intervention effects of the extract of Alismatis Rhizoma on streptozotocin-induced type 2 diabetic rats

计量

出版历程

目录