Abstract: To enhance the quality control of tebipenem pivoxil and establish its quality criteria, the synthetic route of tebipenem pivoxil was analyzed and five related substances(P1, P2, P6, P8 and P9)were synthesized and characterized by ¹H NMR and MS. The purities of the related substances were over 95% via HPLC detection. The target compounds can be used as the reference of the related substances in the quality control of tebipenem pivoxil. The starting materials were cheap and easy to obtain; the reaction conditions were mild.