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紫花地丁与长萼堇菜的傅里叶变换红外光谱鉴别

贺雨馨, 孙志蓉, 曲继旭, 程丽丽, 朱南南

贺雨馨, 孙志蓉, 曲继旭, 程丽丽, 朱南南. 紫花地丁与长萼堇菜的傅里叶变换红外光谱鉴别[J]. 中国药科大学学报, 2018, 49(3): 310-316. DOI: 10.11665/j.issn.1000-5048.20180309
引用本文: 贺雨馨, 孙志蓉, 曲继旭, 程丽丽, 朱南南. 紫花地丁与长萼堇菜的傅里叶变换红外光谱鉴别[J]. 中国药科大学学报, 2018, 49(3): 310-316. DOI: 10.11665/j.issn.1000-5048.20180309
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HE Yuxin, SUN Zhirong, QU Jixu, CHENG Lili, ZHU Nannan. Identification of Viola yedoensis Makino and Viola inconspicua by FT-IR[J]. Journal of China Pharmaceutical University, 2018, 49(3): 310-316. DOI: 10.11665/j.issn.1000-5048.20180309
Citation: HE Yuxin, SUN Zhirong, QU Jixu, CHENG Lili, ZHU Nannan. Identification of Viola yedoensis Makino and Viola inconspicua by FT-IR[J]. Journal of China Pharmaceutical University, 2018, 49(3): 310-316. DOI: 10.11665/j.issn.1000-5048.20180309
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紫花地丁与长萼堇菜的傅里叶变换红外光谱鉴别

  • 北京中医药大学中药学院

基金项目: 北京中医药大学民族医药研究专项资助项目(No.2015-JYB-JSMS025)；北京中医药大学研究生项目资助项目(No.2015-JYB-XS065)

Identification of Viola yedoensis Makino and Viola inconspicua by FT-IR

摘要

    摘要
  • 摘要: 应用傅里叶变换红外光谱法对紫花地丁与长萼堇菜的提取物及不同萃取部位进行结构分析及鉴别研究。紫花地丁与长萼堇菜的提取物的一维红外谱图显示两者均具有芳香类物质，挥发性物质及糖苷类物质。两者不同萃取部位的二阶导数谱差异较为明显，尤其在970~800 cm-1范围内的自动峰数量和峰强的差别明显。在石油醚萃取部位的二阶导数谱图中，紫花地丁出现了5个自动峰，而长萼堇菜有7个自动峰，且峰强大于紫花地丁；在氯仿萃取部位的二阶导数谱图中，长萼堇菜出现了6个自动峰，而紫花地丁则出现4个自动峰，二者峰强差别较小；在乙酸乙酯萃取部位的二阶导数谱图中，紫花地丁出现了6个自动峰，而长萼堇菜出现了4个自动峰。同时在乙酸乙酯萃取部位的二阶导数红外谱图中紫花地丁最强峰的出峰位置在1 467 cm-1处，而长萼堇菜最强峰出峰位置在1 384 cm-1处，可以将二者区分开。研究表明，一维红外光谱与二阶导数分析方法相结合，能够实现紫花地丁与长萼堇菜的区分鉴别，该法准确可靠，为紫花地丁及其他混淆品的鉴别提供新思路和新方法。
    Abstract: The different extracts and of Viola yedoensis Makino and Viola inconspicua were analyzed and identified by Fourier transform infrared spectroscopy(FT-IR). One-dimensional infrared spectrum showed that the extracts of Viola yedoensis Makino and Viola inconspicua contained the aromatics, volatile substances and glycosides, with not significant differences from each other. However, different extraction sites of the two medicinal materials in second derivation spectrum were obviously different, especially the number of automatic peaks and peak intensity in the range of 970- 800 cm-1. Viola yedoensis Makino displayed 5 automatic peaks, 6 automatic peaks and 6 automatic peaks, while Viola inconspicua displayed 7 automatic peaks, 4 peaks, 4 peaks in the second derivation spectrum of petroleum ether extraction site, chloroform extraction site and the ethyl acetate extraction site. In addition, the peak position of the strongest peak in the second derivative of the ethyl acetate extraction site was 1 467 cm-1, while the strongest peak of the Viola inconspicua was at 1 384 cm-1, so the two medicinal materials can be distinguished by the strongest peak position of ethyl acetate extraction site in second derivation spectrum. Studies demonstrated that one-dimensional infrared spectroscopy combined with the second-order derivative analysis could achieve the accurate identification between Viola yedoensis Makino and Viola inconspicua. This research provides new ideas and new methods for the identification of Viola and other adulterants.
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  • [1] Chinese Pharmacopoeia Commission.Chinese Pharmacopoeia:part 1(中华人民共和国药典:一部)[S].Beijing:China Medical Science Press,2015:337-338.
    [2] Cheng LL,Sun ZR,Zhu NN,et al.Research Status of Pharmacology of Viola yedoensis Makino[C].China Commodities Society Academic Forum(中国商品学会学术论坛).2015.
    [3] Zhang YY,Hou HC,Yang JY.Comparison of Viola yedoensis Makino and its common counterfeit[J].Mod Chin Med(中国现代中药),2010,12(7):25-27.
    [4] Zhu Y.Studies on pharmacognosy of Violae herba and adulterant(紫花地丁及其常见混淆品的生药学研究)[D].Luzhou:Southwest Medical University,2014.
    [5] Yang YR,Liu F.Study of dendrobium from Yunnan by Fourier transform infrared spectroscopy combined with hierarchical cluster analysis[J].Chin J Inform Tradit Chin Med(中国中医药信息杂志),2014,21(9):75-79.
    [6] Li XN,Liu XP,Li QR,et al.FTIR spectrum identification of Hedyotis diffusa and its counterfeit[J].J Chin Med Mater(中药材),2004,27(5):335-337.
    [7] Zhang DY,Chen JT,Wang MF,et al.Identification of quality goods and adulterants of traditional Chinese medicine Lygodium japonicum(Thumb.)Sw by FT-IR[J].J Anhui Agric Sci(安徽农业科学),2017,45(10):115-117.
    [8] Zhou Y,Li PF,Zhang QW et al.Study on the identification of partial Herba Japonicum by Fourier transform infrared spectroscopy[J].Spectrosc Spectral Anal(光谱学与光谱分析),2013,33(7):1791-1795.
    [9] Li YW,Zhou FQ,Wang LP,et al.FTIR fingerprint identification of Ziziphus jujuba and its counterfeit[J].Chin Arch Tradit Chin Med(中华中医药学刊),2005,23(4):713-715.
    [10] Li YM,Gong Y,Jiang YP.Determination of volatile components in different parts of Viola inconspicus in Guizhou[J].Guizhou Agric Sci(贵州农业科学),2017,45(3):14-17.
    [11] Cao J.Qualitative and qualitative studies on the flavone glycosides of Violal yedoensis(紫花地丁黄酮苷类成分的定性定量研究)[D].Shanghai:Fudan University,2013.
    [12] Chen HL,Sun J,Qin FF,et al.Studies on chemical constituents of petroleum ether extract of Viola yedoensis Makino.by GC-MS chromatogram[J].J Chengdu Univ Tradit Chin Med(成都中医药大学学报),2013,36(1):50-52.
    [13] Liu H,Hu W,Fang Y.Chemical constituents in ethyl acetate fraction from Viola yedoensis[J].Anhui Med Pharm J(安徽医药),2015(6):1068-1071.
    [14] Gong Y,Jiang YP,Li YM.Determination of total flavonoids in Viola inconspicua[J].J Qiannan Med Coll Nationalities(黔南民族医专学报),2016,29(3):157-159.
    [15] Chen YS.Taxonomic revision of Viola L.in China(中国堇菜属的分类修订)[D].Beijing:Graduate University of Chinese Academy of Sciences,2006.
    [16] Zhu JJ,Chen XL.Study on the Classification of Viola yedoensis in Hangzhou and its similar species[J].Zhejiang J Tradit Chin Med(浙江中医杂志),2010,45(1):54-55.
    [17] Yang Y,Zhang XF,Jiang H,et al.Assay of flavonoids and trace elements in Viola yedoensis Makino from different habitats[J].Chin J Hosp Pharm(中国医院药学杂志),2016,36(5):342-346.
    [18] Mai X,Ouyang T,Cao YS,et al.Application of second derivative IR fingerprint spectra to origin prediction of the TCM of Viola yedoensis Makino[J].Phys Test Chem Anal B:Chem Anal(理化检验-化学分册),2011,47(1):12-14.
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  • 刊出日期:  2018-06-24

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