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miR-29a促进人乳腺癌MCF-7细胞体外迁移和侵袭的机制

吴悠, 汤婷婷, 朱琴华, 金亮, 潘怡

吴悠, 汤婷婷, 朱琴华, 金亮, 潘怡. miR-29a促进人乳腺癌MCF-7细胞体外迁移和侵袭的机制[J]. 中国药科大学学报, 2018, 49(3): 348-353. DOI: 10.11665/j.issn.1000-5048.20180314
引用本文: 吴悠, 汤婷婷, 朱琴华, 金亮, 潘怡. miR-29a促进人乳腺癌MCF-7细胞体外迁移和侵袭的机制[J]. 中国药科大学学报, 2018, 49(3): 348-353. DOI: 10.11665/j.issn.1000-5048.20180314
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WU You, TANG Tingting, ZHU Qinhua, JIN Liang, PAN Yi. Mechanisms of miR-29a in migration and invasion of breast cancer MCF-7 cells in vitro[J]. Journal of China Pharmaceutical University, 2018, 49(3): 348-353. DOI: 10.11665/j.issn.1000-5048.20180314
Citation: WU You, TANG Tingting, ZHU Qinhua, JIN Liang, PAN Yi. Mechanisms of miR-29a in migration and invasion of breast cancer MCF-7 cells in vitro[J]. Journal of China Pharmaceutical University, 2018, 49(3): 348-353. DOI: 10.11665/j.issn.1000-5048.20180314
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miR-29a促进人乳腺癌MCF-7细胞体外迁移和侵袭的机制

  • 中国药科大学生命科学与技术学院

基金项目: 国家高技术研究发展计划(“八六三”计划)资助项目(No.2015AA020314)；国家自然科学基金资助项目(No.81570696,No.81702941)；江苏省高校“青蓝工程”资助项目；中央高校基本科研业务费专项资金资助项目(No.2632017PY06)

Mechanisms of miR-29a in migration and invasion of breast cancer MCF-7 cells in vitro

摘要

    摘要
  • 摘要: 探讨微小核酸miRNA-29a对人乳腺癌细胞MCF-7体外迁移和侵袭的促进作用及其机制。采用miR-29a模拟物及miR-29a抑制剂分别上调和下调miR-29a的表达；采用细胞划痕法和Transwell小室法检测miR-29a对细胞迁移和侵袭能力的影响；采用Targetscan7.1数据库预测miR-29a的靶基因；荧光素酶报告法验证miR-29a的靶基因；Western blot和实时荧光定量PCR验证其表达结果。结果表明:miR-29a可显著促进MCF-7细胞的迁移和侵袭能力。Targetscan软件预测HBP1 可能为miR-29a的靶基因，荧光素酶报告实验显示miR-29a靶向HBP1 基因的3′-UTR区。Western blot和实时荧光定量PCR结果显示，miR-29a下调了HBP1蛋白水平的表达，而mRNA水平则无明显变化。研究结果揭示在乳腺癌中高表达的miR-29a通过下调HBP1，从而使乳腺癌细胞获得高的迁移和侵袭能力，促进乳腺癌的转移。
    Abstract: The aim of this study was to investigate the effect and mechanisms of miR-29a in migration and invasion of human breast cancer MCF-7 cells in vitro. MCF-7 cells were treated with miR-29a mimic or miR-29a inhibitor to up-regulate/down-regulate the expression level of miR-29a. Wound-healing assay and transwell chamber were employed to determine cell migration and invasion in vitro. The target gene of miR-29a was predicted with the Targetscan7. 1 database and verified through luciferase reporter method. The effects of miR-29a on the expression of the potential target were detected by Western blot and real-time PCR. Results showed that in vitro migration and invasion ability of MCF-7 cells was increased significantly by miR-29a, which could target HBP1 in the 3′-UTR region. The protein expression of HBP1 was decreased by miR-29a overexpression. However, the alteration of miR-29a had no significant effect on the expression of HBP1 mRNA. The results validated that miR-29a, highly expressed in breast cancer, could down-regulate HBP1, which in turn promotes migration and invasion ability of breast cancer cells, thus promoting breast cancer metastasis.
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  • [1] Redig AJ,McAllister SS.Breast cancer as a systemic disease:a view of metastasis[J].J Intern Med,2013,274(2):113-126.
    [2] Zhang K,Zhang Y,Liu C,et al.MicroRNAs in the diagnosis and prognosis of breast cancer and their therapeutic potential[J].Int J Oncol,2014,45(3):950-958.
    [3] Di Leva G,Garofalo M,Croce CM.microRNAs in cancer[J].Annu Rev Pathol,2014,9:287-314.
    [4] Pei YF,Lei Y,Liu XQ.MiR-29a promotes cell proliferation and EMT in breast cancer by targeting ten eleven translocation 1[J].Biochim Biophys Acta,2016,1862(11):2177-2185.
    [5] Rostas JW,Pruitt HC,Metge BJ,et al.microRNA-29 negatively regulates EMT regulator N-myc interactor in breast cancer[J].Mol Cancer,2014,13:200.
    [6] Shukla GC,Singh J,Barik S.MicroRNAs:processing,maturation,target recognition and regulatory functions[J].Mol Cell Pharmacol,2011,3(3):83-92.
    [7] Debacker K,Kooy RF.Fragile sites and human disease[J].Hum Mol Genet,2007,16(2):150-158.
    [8] Chen L,Xiao H,Wang ZH,et al.miR-29a suppresses growth and invasion of gastric cancer cells in vitro by targeting VEGF-A[J].BMB Rep,2014,47(1):39-44.
    [9] Tréhoux S,Lahdaoui F,Delpu Y,et al.Micro-RNAs miR-29a and miR-330-5p function as tumor suppressors by targeting the MUC1 mucin in pancreatic cancer cells[J].Biochim Biophys Acta,2015,1853(10 Pt A):2392-2403.
    [10] Yamamoto N,Kinoshita T,Nohata N,et al.Tumor-suppressive microRNA-29a inhibits cancer cell migration and invasion via targeting HSP47 in cervical squamous cell carcinoma[J].Int J Oncol,2013,43(6):1855-1863.
    [11] Pasqualini L,Bu H,Puhr M,et al.miR-22 and miR-29a are members of the androgen receptor cistrome modulating LAMC1 and Mcl-1 in prostate cancer[J].Mol Endocrinol,2015,29(7):1037-1054.
    [12] Liu C,Duan P,Li B,et al.miR-29a activates Hes1 by targeting Nfia in esophageal carcinoma cell line TE-1[J].Oncol Lett,2015,9(1):96-102.
    [13] Tang W,Zhu Y,Gao J,et al.MicroRNA-29a promotes colorectal cancer metastasis by regulating matrix metalloproteinase 2 and E-cadherin via KLF4[J].Br J Cancer,2014,110(2):450-458.
    [14] Shih HH,Xiu M,Berasi SP,et al.HMG box transcriptional repressor HBP1 maintains a proliferation barrier in differentiated liver tissue[J].Mol Cell Biol,2001,21(17):5723-5732.
    [15] Huang JS, Xiao R, Pang Y, et al. HBP1—an important tumor inhibitor[J].Chin J Biochem Mol Biol(中国生物化学与分子生物学报),2012,28(10):913-918.
    [16] Paulson KE,Rieger-Christ K,McDevitt MA,et al.Alterations of the HBP1 transcriptional repressor are associated with invasive breast cancer[J].Cancer Res,2007,67(13):6136-6145.
    [17] Yee AS,Paulson EK,McDevitt MA,et al.The HBP1 transcriptional repressor and the p38 MAP kinase:unlikely partners in G1 regulation and tumor suppression[J].Gene,2004,336(1):1-13.
    [18] Sampson EM,Haque ZK,Ku MC,et al.Negative regulation of the Wnt/b-catenin pathway by the transcriptional repressor HBP1[J].EMBO J,2001,20(16):4500-4511.
    [19] Qin X,Zhang H,Zhou X,et al.Proliferation and migration mediated by Dkk-1/Wnt/b-catenin cascade in a model of hepatocellular carcinoma cells[J].Transl Res,2007,150(5):281-294.
    [20] Wang Z,Ma Q.Beta-catenin is a promising key factor in the SDF-1/CXCR4 axis on metastasis of pancreatic cancer[J].Med Hypotheses,2007,69(4):816-820.
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  • 刊出日期:  2018-06-24

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