Abstract:
A qualitative analysis was developed to identify different ingredients from three
Ginkgo biloba preparations(including
Yinxingye Diwan, extract of
Ginkgo biloba leaves tablets
and Yinxingtongzhi Diwan)based on high resolution mass spectrometry and metabolomics technology. An XSELECT HSS T3(4. 6 mm×150 mm, 3. 5 μm)column was used for separation, with the mobile phases consisting of acetonitrile and water containing 0. 1% formic acid. The column temperature was set at 40 °C. Negative ion mode was used for mass spectrometric data acquisition. Through partial least squares projection to latent structure-discriminant analysis(PLS-DA), we firstly found the different compounds among different
Ginkgo biloba preparations. Subsequently, the compounds displaying different abundance levels via database searching and literature matching were identified. Finally, we identified 21 different compounds between the extract of
Ginkgo biloba leaves tablets and
Yinxingye Diwan, which mostly belong to the organic acids and flavonols families. Quantitative analysis showed that the ingredients which had higher abundance in extract of
Ginkgo biloba leaves tablets were mainly organic acids, whereas those exhibiting higher levels in
Yinxingye Diwan are flavonols
. We also identified 12 different ingredients between the
Yinxingye Diwan and
Yinxingtongzhi Diwan, which were flavonols, sciadopitysin and organic acids. The results of this study are useful for studying different chemical constituents from distinct
Ginkgo biloba preparations.