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金露梅提取物对α-淀粉酶、α-葡萄糖苷酶和醛糖还原酶的抑制作用

李美华, 王渭清, 曾阳, 郭凤霞, 严培瑛, 李锦萍

李美华, 王渭清, 曾阳, 郭凤霞, 严培瑛, 李锦萍. 金露梅提取物对α-淀粉酶、α-葡萄糖苷酶和醛糖还原酶的抑制作用[J]. 中国药科大学学报, 2018, 49(4): 470-475. DOI: 10.11665/j.issn.1000-5048.20180413
引用本文: 李美华, 王渭清, 曾阳, 郭凤霞, 严培瑛, 李锦萍. 金露梅提取物对α-淀粉酶、α-葡萄糖苷酶和醛糖还原酶的抑制作用[J]. 中国药科大学学报, 2018, 49(4): 470-475. DOI: 10.11665/j.issn.1000-5048.20180413
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LI Meihua, WANG Weiqing, ZENG Yang, GUO Fengxia, YAN Peiying, LI Jinping. Inhibition of Potentilla fruticosa extracts on α-amylase, α-glucosidase and aldose reductase[J]. Journal of China Pharmaceutical University, 2018, 49(4): 470-475. DOI: 10.11665/j.issn.1000-5048.20180413
Citation: LI Meihua, WANG Weiqing, ZENG Yang, GUO Fengxia, YAN Peiying, LI Jinping. Inhibition of Potentilla fruticosa extracts on α-amylase, α-glucosidase and aldose reductase[J]. Journal of China Pharmaceutical University, 2018, 49(4): 470-475. DOI: 10.11665/j.issn.1000-5048.20180413
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金露梅提取物对α-淀粉酶、α-葡萄糖苷酶和醛糖还原酶的抑制作用

  • 1.

    青海师范大学生命科学学院

  • 2.

    青海省产品质量监督检测所

  • 3.

    浙江大学城市学院

基金项目: 青海省自然科学基金资助项目(No.2015-ZJ-907)；青藏高原药用动植物资源重点实验室资助项目(No.2017-z-y25)；浙江大学城市学院教师科研基金资助项目(No.J-15002)

Inhibition of Potentilla fruticosa extracts on α-amylase, α-glucosidase and aldose reductase

摘要

    摘要
  • 摘要: 通过α-淀粉酶抑制剂、α-葡萄糖苷酶抑制剂和醛糖还原酶抑制剂体外筛选模型，研究金露梅水提物及95%甲醇提取石油醚萃取部位、乙酸乙酯萃取部位和水层部位对α-淀粉酶、α-葡萄糖苷酶和醛糖还原酶的抑制活性作用；通过糖耐量实验测定金露梅提取物对小鼠餐后血糖的影响。结果显示，金露梅水提物及95%甲醇提取石油醚萃取部位、乙酸乙酯萃取部位对α-淀粉酶的半数抑制浓度(IC50)分别为0.432、1.193、0.507 mg/mL；对α-葡萄糖苷酶的IC50分别为0.164、0.768、0.466 mg/mL；对醛糖还原酶的IC50分别为0.742、2.158、1.098 mg/mL。结果表明，金露梅水提物和金露梅乙酸乙酯萃取部位对α-淀粉酶、α-葡萄糖苷酶和醛糖还原酶的抑制活性较高。同时，金露梅水提物与乙酸乙酯萃取部位均能显著降低小鼠的餐后血糖水平。
    Abstract: This study measured the in vitro inhibitory effects of α-amylase(AM), α-glycosidase(AG)and aldose reductase(AR)extraction from Potentilla fruticosa in three solvents: water extract(WE)and 95% methanol extraction of petroleum ether part(MEP), 95% methanol extraction of ethyl acetate part(MEE)and 95% methanol extraction of water part(MEW)through α-amylase inhibitors(AMI), α-glycosidase inhibitors(AGI)and aldose reductase inhibitors(ARI)activity screening models. In vivo effects of different solvents from Potentilla fruticosa on impaired glucose tolerance of mice were also measured. Among them, WE, MEP and MEE exhibited against AMI activity with IC50 values of 0. 432, 1. 193 and 0. 507 mg/mL, respectively. Three solvents against AGI activity with IC50 values of 0. 164, 0. 768 and 0. 466 mg/mL, respectively. Three solvents against ARI activity with IC50 values of 0. 742, 2. 158 and 1. 098 mg/mL, respectively. The study suggests that Potentilla fruticosa in water extract and 95% methanol extraction of ethyl acetate part demonstrated a stronger inhibitory effect on AM, AG and AR. Meanwhile, Potentilla fruticosa in water extract and 95% methanol extraction of ethyl acetate part can be significantly decreased the postprandial blood glucose in mice.
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  • 刊出日期:  2018-08-24

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