金水宝胶囊对尘肺模型大鼠的改善作用及其机制
Mechanism of JinShuiBao capsule in ameliorating rat pneumoconiosis model
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摘要: 通过建立大鼠尘肺模型，探讨金水宝胶囊改善呼吸功能及肺组织病变的相关机制。通过气管注射石英粉尘建立大鼠慢性尘肺模型。造模成功后，治疗组灌胃给予金水宝胶囊600、300 mg/kg，每天1次，连续6个月。给药的第1、3、6月，各组分别取6只大鼠，进行血液流变学、血管内皮功能、免疫炎症因子、氧化应激等指标检测。结果显示，在6个月试验期间，金水宝胶囊高剂量对于各时间点血浆黏度具有明显的改善作用(P<；0.05)，对全血黏度有部分改善作用。金水宝胶囊可降低血清TGF-β、TNF-α和IL-6等炎症因子水平(P<；0.05，P<；0.01)，高剂量能显著降低血清CD4+/CD8+水平(P<；0.05，P<；0.01)。金水宝胶囊可明显降低模型大鼠血清MDA水平，并升高SOD活力(P<；0.05)，具有剂量相关性。金水宝胶囊剂量依赖性降低模型大鼠支气管肺泡灌洗液中白细胞数量(P<；0.05，P<；0.01)。金水宝胶囊高剂量全部试验周期内对支气管肺泡灌洗液ET、NO和PC水平均有明显改善作用(P<；0.05，P<；0.01)，而低剂量第1月具有部分改善作用，而3月后具有统计学显著差异的改善作用。阳性药汉防己甲素片对炎症相关指标具有较好改善作用，但对于血流变和氧化应激指标则未见明显影响。实验结果表明，金水宝胶囊对于尘肺模型大鼠的改善作用涉及血液黏度、全身及肺组织炎症反应、血管内皮损伤、机体氧化应激状态、肺组织纤维化等多方面的作用机制。Abstract: To investigate the mechanism of JinShuiBao capsule on improving respiratory function and lung tissue pathology in rat pneumoconiosis model. Chronic pneumoconiosis rat model was established by tracheal injection of quartz dust. JinShuiBao was administrated orally by 600 and 300 mg/kg, once daily for 6 months. At the 1st, 3rd and 6th month of administration, 6 rats in each group were taken for hemorheology, vascular endothelial function, immunoinflammatory cytokines and oxidative stress. The results showed that high dose of JinShuiBao had a significant improvement on the plasma viscosity at each time point(P< 0. 05)during the 6-month trial, and partially improved the whole blood viscosity. Both dose of JinShuiBao capsule significantly decreased the levels of serum inflammatory cytokines such as TGF-β, TNF-α and IL-6(P< 0. 05, P< 0. 01), and high dose group could significantly decrease the level of CD4+/CD8+(P< 0. 01). The high dosage of JinShuiBao could obviously reduce the level of serum MDA and increase the activity of SOD(P< 0. 05), and obviously reduced the number of leukocytes in the bronchoalveolar lavage fluid of model rats. In the high-dose group, the levels of ET, NO and PC in bronchoalveolar lavage fluid were significantly improved in all the experimental periods(P< 0. 05, P< 0. 01), while the low-dose group also had a statistically significant improvement at 3 month later. These results suggested that the improvement of JinShuiBao capsule on pneumoconiosis rats involved various mechanism, including blood viscosity, systemic and pulmonary inflammatory response, vascular endothelial injury, and oxidative stress in the whole body and lung fibrosis.
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Keywords:
- JinShuiBao capsule /
- pneumoconiosis /
- pulmonary fibrosis /
- mechanism of action /
- immune regulation /
- hemorrheology
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