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不同产地脱脂乳木果仁总酚含量测定及其生物活性

Total phenolic contents and bioactivities of defatted Vitellaria paradoxa kernels from different origin

  • 摘要: 测定了26个不同产地脱脂乳木果仁甲醇提取物的总酚含量(TPC)、抗氧化、细胞毒、EB病毒早期抗原(EBV-EA)抑制等活性,并评价了TPC与各生物活性间的相关性。采用紫外分光光度法测定TPC;采用1,1-二苯基-2-苦基肼(DPPH)自由基清除法、2,2′-连氮基-双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS)自由基清除法和铁离子还原法(FRAP)3种方法评价提取物抗氧化活性;3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)法评价提取物对人白血病细胞HL60,肺癌细胞A549和乳腺癌细胞SK-BR-3的细胞毒性;间接免疫酶法评价提取物对EBV-EA抑制活性。结果表明:不同产地乳木果仁总酚含量相差较大(25.8~265.7 mg/g,以没食子酸计);部分产地提取物或有较好的抗氧化活性[DPPH:IC50 3.4~54.9 μg/mL,ABTS:1.11~4.09 mmol/g(以Trolox计),FRAP:1.24~1.93 mmol/g(以Trolox计)]、较强的细胞毒性(对不同细胞)(IC50 25.1~95.5 μg/mL)和EBV-EA抑制活性(100 μg/mL:85.8%~94.9%),其中TPC只与抗氧化活性有显著的相关关系(r=0.750~0.837,P<;0.01)。

     

    Abstract: Total phenolic contents, antioxidant, cytotoxic and Epstein-Barr virus early antigen(EBV-EA)inhibitory activities of twenty-six methanol extracts from defatted Vitellaria paradoxa kernel at different sites were investigated. Furthermore, the correlation between total phenolic contents and bioactivities was discussed. The total phenolic contents were determined spectrometrically and antioxidant activities were evaluated by 1, 1-diphenyl-2-picrylhydrazyl(DPPH), 2, 2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid(ABTS)radical scavenging activity and ferric-reducing antioxidant power(FRAP)methods. The cytotoxic activities against human leukemia HL60 cells, lung cancer A549 cells and breast cancer SK-BR-3 cells were assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-2H-tetrazolium bromide(MTT)assay and the inhibitory effect on EBV-EA was assayed by the indirect immunofluorescence technique. Results showed that the total phenolic contents of twenty-six methanol extracts were different(25. 8 - 265. 7 mg GAE/g DW). In addition, some of the extracts had potent antioxidant activities(DPPH: IC50 3. 4 - 54. 9 μg/mL, ABTS: 1. 11- 4. 09 mmol TE/g, FRAP: 1. 24 - 1. 93 mmol TE/g), toxicities against one or more of HL60, A549 and SK-BR-3 cells(IC50 25. 1- 95. 5 μg/mL)and inhibitory effect on EBV-EA(85. 8%- 94. 9% at 100 μg/mL). A significant correlation was observed between total phenolic contents and antioxidant activities(r=0. 750-0. 837, P< 0. 01).

     

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