Abstract:
The phytochemical studies on the leaves of
Anisopus mannii led to the isolation of seven compounds by silica gel, ODS, DIAION HP-20, Sephadex LH-20 colunmn chromatographer, their structures were elucidated on the basis of the spectroscopic analyses(NMR, HRMS)and the comparisons with the literatures as 3
β-acetoxylup-20(29)-ene(
1 ), 1-acetoxy-2-isopropyl-1-tridecene(
2 ), rutin(
3 ), 3, 6′-diferuloylsucrose(
4 ), 3-
O-
β-D-glucopyranosyl-3-
β-hydroxyolean-12-en-28-oic acid 28-
O-[
α-L-rhamnopyra- nosyl-(1→2)-
β-D-glucopyranosyl] ester(
5 ), conduritol A(
6 ), hoyacarnoside I(
7 ). Meanwhile, the isolated compounds were evaluated for their inhibitory activities against melanogensis in B16 melanoma cells, as the results, all compounds exhibited melanogenesis-inhibitory activity and compound
5 showed a strongest activity(Melanin content: (27. 4±3. 5)%, Cell Viability: (54. 9±5. 6)% with a concentration of 30 μmol/L)which could be further developed.