白鲜皮化学成分及其α-葡萄糖苷酶抑制活性
Chemical constituents extracted from Dictamnus dasycarpus and their α-glucosidase inhibitory activity
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摘要: 采用硅胶、凝胶柱色谱方法对白鲜皮(Dictamnus dasycarpus)的化学成分进行分离纯化。从其石油醚部位分离得到了5个化合物，利用IR、MS和NMR等技术对分离得到的化合物进行了结构鉴定。5个化合物的结构分别为:二十二烷醇( 1 )、2，2-二二苯甲基-3，3-二苯基丙酸乙酯( 2 )、柠檬苦素( 3 )、黄柏酮( 4 )和白鲜碱( 5 )。其中化合物 2 为新化合物，化合物 1 首次从该植物中分离得到。采用PNPG 法筛选化合物的α-葡萄糖苷酶抑制活性。结果表明化合物 3 ~ 5 表现出较好的α-葡萄糖苷酶的抑制活性。该结果为白鲜皮的深度开发利用提供了理论依据。Abstract: Five compounds were isolated from the petroleum ether extract of Dictamnus dasycarpus using various chromatographic techniques, such as column chromatography over silica gel and Sephadex LH-20. Their structures were elucidated by spectroscopic data(IR, MS, NMR), which were identified as docosanol( 1 ), ethyl 2, 2-dibenzhydryl-3, 3-di-phenylpropionate( 2 ), limonin( 3 ), obacunone( 4 )and dictamnine( 5 ). Compound 2 was a new compound and compound 1 was isolated from this plant for the first time. PNPG method was used to determine α-glucosidase inhibitory activity of these compounds. The results indicated that compounds 3 - 5 possessed stronger inhibitory activities than the positive control of acarbose, which provided a theoretical basis for further development and utilization of Dictamnus dasycarpus.
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Keywords:
- Dictamnus dasycarpus /
- chemical constituents /
- α-glucosidase /
- inhibitory activity /
- diabetes
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