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近红外二区成像载药脂质体的制备、体外成像及抗肿瘤活性

王紫安, 刘洋, 王鹏, 蒋逸飞, 吉民

王紫安, 刘洋, 王鹏, 蒋逸飞, 吉民. 近红外二区成像载药脂质体的制备、体外成像及抗肿瘤活性[J]. 中国药科大学学报, 2019, 50(4): 438-443. DOI: 10.11665/j.issn.1000-5048.20190408
引用本文: 王紫安, 刘洋, 王鹏, 蒋逸飞, 吉民. 近红外二区成像载药脂质体的制备、体外成像及抗肿瘤活性[J]. 中国药科大学学报, 2019, 50(4): 438-443. DOI: 10.11665/j.issn.1000-5048.20190408
WANG Zian, LIU Yang, WANG Peng, JIANG Yifei, JI Min. Preparation, in vitro imaging and antitumor activity of the second near-infrared imaging drug-loaded liposomes[J]. Journal of China Pharmaceutical University, 2019, 50(4): 438-443. DOI: 10.11665/j.issn.1000-5048.20190408
Citation: WANG Zian, LIU Yang, WANG Peng, JIANG Yifei, JI Min. Preparation, in vitro imaging and antitumor activity of the second near-infrared imaging drug-loaded liposomes[J]. Journal of China Pharmaceutical University, 2019, 50(4): 438-443. DOI: 10.11665/j.issn.1000-5048.20190408

近红外二区成像载药脂质体的制备、体外成像及抗肿瘤活性

基金项目: 国家自然科学基金资助项目(No.81671745)

Preparation, in vitro imaging and antitumor activity of the second near-infrared imaging drug-loaded liposomes

  • 摘要: 通过纳米技术实现诊断与治疗的结合,有利于推动肿瘤治疗的发展。近红外二区(NIR-II)成像技术由于其成像上的优势在近年来发展迅速。本研究制备了一种新型纳米载药系统——DOX-IR1061阳离子脂质体,载入NIR-II荧光探针IR1061与阿霉素(DOX)分别作为成像剂和治疗剂,并且通过十八胺增强其细胞摄取及肿瘤细胞抑制能力。通过对脂质体的NIR-II性能测试表明,DOX-IR1061阳离子脂质体具有NIR-II成像能力;对脂质体细胞摄取行为的分析和肿瘤细胞抑制实验证明,十八胺能够促进脂质体被细胞摄取,与DOX协同增强抗肿瘤效果。研究结果表明,DOX-IR1061阳离子脂质体具有同时实现肿瘤成像与治疗的潜力,具有进一步研究的价值。
    Abstract: The combination of diagnosis and treatment through nanotechnology is conducive to the development of cancer treatment. Fluorescent imaging in the second near-infrared window(NIR-II)developed rapidly in recent years due to its imaging advantages. In this paper, we prepared a novel nano drug system, DOX-IR1061-cationic liposome, in which NIR-II fluorescent probe IR1061 was loaded as imaging agents and doxorubicin was loaded as therapeutic agents. It also explores enhanced cellular uptake and cancer cell inhibition rate through octadecylamine. Our NIR-II performance test on liposomes showed that DOX-IR1061-cationic liposome has NIR-II imaging ability. Analysis of liposome cell uptake behavior and cancer cell inhibition experiments demonstrated that octadecylamine can promote liposome uptake by cells and synergize with DOX to enhance anticancer effects. This suggests that the DOX-IR1061-cationic liposome can be used to achieve imaging and therapy effect with further research value.
  • [1] Nakamura Y,Mochida A,Choyke PL,et al.Nanodrug delivery:is the enhanced permeability and retention effect sufficient for curing cancer [J]?Bioconjug Chem,2016,27(10):2225-2238.
    [2] Longmire MR,Ogawa M,Choyke PL,et al.Biologically optimized nanosized molecules and particles:more than just size[J].Bioconjug Chem,2011,22(6):993-1000.
    [3] Liu HP,Xi Q,Zhou JP,et al.Preparation of paclitaxel-loaded polymeric nanoparticles and evaluation on their anti-tumor activities[J].J China Pharm Uni(中国药科大学学报),2013,44(3):228-233.
    [4] Stylianopoulos T,Jain RK.Design considerations for nanotherapeutics in oncology[J].Nanomed:Nanotechnol Biol Med,2015,11(8):1893-1907.
    [5] Jain RK.Normalization of tumor vasculature:an emerging concept in antiangiogenic therapy[J].Science,2005,307(5706):58-62.
    [6] Prabhakar U,Maeda H,Jain RK,et al.Challenges and key considerations of the enhanced permeability and retention effect for nanomedicine drug delivery in oncology[J].Cancer Res,2013,73(8):2412-2417.
    [7] Ma YF,Huang J,Song SJ,et al.Cancer-targeted nanotheranostics:recent advances and perspectives[J].Small,2016,12(36):4936-4954.
    [8] Antaris AL,Chen H,Cheng K,et al.A small-molecule dye for NIR-II imaging[J].Nat Mater,2016,15(2):235-242.
    [9] Hong GS,Antaris AL,Dai HJ.Near-infrared fluorophores for biomedical imaging[J].Nat Biomed Eng,2017,1(1):0010.
    [10] Vahrmeijer AL,Hutteman M,van der Vorst JR,et al.Image-guided cancer surgery using near-infrared fluorescence[J].Nat Rev Clin Oncol,2013,10(9):507-518.
    [11] Tao ZM,Hong GS,Shinji C,et al.Biological imaging using nanoparticles of small organic molecules with fluorescence emission at wavelengths longer than 1000 nm[J].Angew Chem Int Ed Engl,2013,52(49):13002-13006.
    [12] Pattni BS,Chupin VV,Torchilin VP.New developments in liposomal drug delivery[J].Chem Rev,2015,115(19):10938-10966.
    [13] De M,Ghosh S,Sen T,et al.A novel therapeutic strategy for cancer using phosphatidylserine targeting stearylamine-bearing cationic liposomes[J].Mol Ther Nucleic Acids,2018,10:9-27.
    [14] Nagao Y,Osawa T,Kozawa K,et al.Synthesis and properties of heptamethine dyes having thiopyran rings[J].J Jpn Soc Colour Mater,2005,78(1):12-16.
    [15] Schmedes A,Hφlmer G.A new thiobarbituric acid(TBA)method for determining free malondialdehyde(MDA)and hydroperoxides selectively as a measure of lipid peroxidation[J].J Am Oil Chem Soc,1989,66(6):813-817.
    [16] Kreuter J.Nanoparticulate systems for brain delivery of drugs[J].Adv Drug Deliv Rev,2001,47(1):65-81.
    [17] Lei M,Ma GL,Sha SJ,et al.Dual-functionalized liposome by co-delivery of paclitaxel with sorafenib for synergistic antitumor efficacy and reversion of multidrug resistance[J].Drug Deliv,2019,26(1):262-272.
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  • 刊出日期:  2019-08-24

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