福白菊化学成分LC-MS<i><sup>n</sup></i>定性分析及HPLC-UV法多成分含量测定

李君彦; 范氏英; 孙中宣; 程雪翔; 叶文才; 汪豪

doi:10.11665/j.issn.1000-5048.20190509

福白菊化学成分LC-MSⁿ定性分析及HPLC-UV法多成分含量测定

1.
中国药科大学中药制药系
2.
湖北凤凰白云山药业有限公司
3.
暨南大学药学院中药及天然药物研究所

基金项目: 珠江人才计划本土创新团队资助项目(No.2017BT01Y036)；国家重点研发计划资助项目(No.2017YFC1703802)

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出版历程
- 刊出日期: 2019-10-24

Qualitative and quantitative analyses of Chrysanthemum morifolium ‘Fubaiju’ by LC-MSⁿ and HPLC-UV

摘要

摘要: 建立LC-MSⁿ法对福白菊化学成分进行定性研究，鉴定了22个化学成分，包括13个黄酮类成分和9个酚酸类成分；采用HPLC-UV法，同一色谱条件下测定福白菊中5种化学成分含量，包括:绿原酸( 1 )、木犀草素-7-O-β-D-葡萄糖苷( 2 )、木犀草素-7-O-β-D-葡萄糖醛酸苷( 3 )、3，5-二咖啡酰基奎宁酸( 4 )、芹菜素-7-O-β-D-葡萄糖苷( 5 )，建立5种化学成分的回归方程，线性关系均良好(r>；0.999 9)，加样回收率为98.72%~103.16%，采用此方法成功检测了10个福白菊样品中上述5种成分的含量。本文建立分析方法简便可靠，可定性及定量分析福白菊中的主要化学成分，适用于福白菊药材的质量分析。
- 福白菊 /
- LC-MSⁿ /
- HPLC-UV /
- 定性分析 /
- 含量测定
Abstract: A qualitative analytical method of liquid chromatography coupled with mass spectrometry(LC-MSⁿ)was developed for the identification of main constituents in Chrysanthemum morifolium ‘Fubaiju’. High-performance liquid chromatography(HPLC)was developed for the quantification of five active components, including chlorogenic acid( 1 ), luteolin-7-O-β-D-glucopyranoside( 2 ), luteolin-7-O-β-D-glucopyranuronide( 3 ), 3, 5-Di-caffeoylquinic acid( 4 ), and apigenin-7-O-β-D-glucopyranoside( 5 ). A total of 22 compounds, including 13 flavonoids and 9 phenolic acids, were identified based on their retention behaviors, UV profiles and MS fragment information. Furthermore, a validation method with good linearity(r> 0. 999 9), precision, stability, repeatability and recovery was successfully applied for simultaneous determination of five major components in 10 batches of C. morifolium ‘Fubaiju’ by HPLC-UV method. The established method was proved to be a validation strategy for the quality evaluation of C. morifolium ‘Fubaiju’.
- Chrysanthemum morifolium ‘Fubaiju’ /
- LC-MSⁿ /
- HPLC-UV /
- qualitative analysis /
- content determination

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参考文献(8)

[1]	Chinese Pharmacopoeia Commission.Chinese Pharmacopoeia:part 1(中华人民共和国药典:一部)[S].Beijing:People′s Medical Publishing House,2015:310.
[2]	Xu L.Research on the pivotal cultivation techniques in GAP and producing area ecological suitability of Fubaiju[D].Wuhan:Hubei University of Chinese Medicine(湖北中医药大学),2015.
[3]	Qu L,Wang T,Dong YZ,et al.Research progress on chemical constituents of Chrysanthemum morifolium and their pharmacologic activities[J].Drug Eval Res(药物评价研究),2015,38(1):98-104.
[4]	Bi SF,Liu MR,Shi XP,et al.Analysis on the chemical constituents of the essential oil from Chrysanthemum morifolium Fubaiju[J].J Beijing Uni Univ(北京联合大学学报),2018,32(4):83-86.
[5]	Lin LZ,Harnly JM.Identification of the phenolic components of chrysanthemum flower(Chrysanthemum morifolium Ramat.)[J].Food Chem,2010,120(1):319-326.
[6]	Zhang WB, Wang ZC, Zhang LY. Determination of 10 caffeoylquinic acids and 22 flavonoids in chrysanthemum samples by ultra-high performance liquid chromatography-diode array detection-tandem mass spectrometry[J].Chin J Anal Chem(分析化学),2013,41(12):1851-1861.
[7]	Melissa MR,Vimal N,Jorge B,et al.UVA,UVB light,and methyl jasmonate,alone or combined,redirect the biosynthesis of glucosinolates,phenolics,carotenoids,and chlorophylls in broccoli sprouts[J].Int J Mol Sci,2017,18(11):2330.
[8]	Xie YY,Qu JL,Wang QL,et al.Comparative evaluation of cultivars of Chrysanthemum morifolium flowers by HPLC-DAD-ESI/MS analysis and antiallergic assay[J].J Agric Food Chem,2012,60(51):12574-12583.

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