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福白菊化学成分LC-MSn定性分析及HPLC-UV法多成分含量测定

Qualitative and quantitative analyses of Chrysanthemum morifolium ‘Fubaiju’ by LC-MSn and HPLC-UV

  • 摘要: 建立LC-MSn法对福白菊化学成分进行定性研究,鉴定了22个化学成分,包括13个黄酮类成分和9个酚酸类成分;采用HPLC-UV法,同一色谱条件下测定福白菊中5种化学成分含量,包括:绿原酸( 1 )、木犀草素-7-O-β-D-葡萄糖苷( 2 )、木犀草素-7-O-β-D-葡萄糖醛酸苷( 3 )、3,5-二咖啡酰基奎宁酸( 4 )、芹菜素-7-O-β-D-葡萄糖苷( 5 ),建立5种化学成分的回归方程,线性关系均良好(r>;0.999 9),加样回收率为98.72%~103.16%,采用此方法成功检测了10个福白菊样品中上述5种成分的含量。本文建立分析方法简便可靠,可定性及定量分析福白菊中的主要化学成分,适用于福白菊药材的质量分析。

     

    Abstract: A qualitative analytical method of liquid chromatography coupled with mass spectrometry(LC-MSn)was developed for the identification of main constituents in Chrysanthemum morifolium ‘Fubaiju’. High-performance liquid chromatography(HPLC)was developed for the quantification of five active components, including chlorogenic acid( 1 ), luteolin-7-O-β-D-glucopyranoside( 2 ), luteolin-7-O-β-D-glucopyranuronide( 3 ), 3, 5-Di-caffeoylquinic acid( 4 ), and apigenin-7-O-β-D-glucopyranoside( 5 ). A total of 22 compounds, including 13 flavonoids and 9 phenolic acids, were identified based on their retention behaviors, UV profiles and MS fragment information. Furthermore, a validation method with good linearity(r> 0. 999 9), precision, stability, repeatability and recovery was successfully applied for simultaneous determination of five major components in 10 batches of C. morifolium ‘Fubaiju’ by HPLC-UV method. The established method was proved to be a validation strategy for the quality evaluation of C. morifolium ‘Fubaiju’.

     

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