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野黄芩素对大鼠急性咽炎的治疗作用

杨阳, 蒋翠花, 金乔梅, 张立邦, 张健, 殷志琦

杨阳, 蒋翠花, 金乔梅, 张立邦, 张健, 殷志琦. 野黄芩素对大鼠急性咽炎的治疗作用[J]. 中国药科大学学报, 2019, 50(5): 600-605. DOI: 10.11665/j.issn.1000-5048.20190514
引用本文: 杨阳, 蒋翠花, 金乔梅, 张立邦, 张健, 殷志琦. 野黄芩素对大鼠急性咽炎的治疗作用[J]. 中国药科大学学报, 2019, 50(5): 600-605. DOI: 10.11665/j.issn.1000-5048.20190514
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YANG Yang, JIANG Cuihua, JIN Qiaomei, ZHANG Libang, ZHANG Jian, YIN Zhiqi. Effect of scutellarein on acute pharyngitis in rats[J]. Journal of China Pharmaceutical University, 2019, 50(5): 600-605. DOI: 10.11665/j.issn.1000-5048.20190514
Citation: YANG Yang, JIANG Cuihua, JIN Qiaomei, ZHANG Libang, ZHANG Jian, YIN Zhiqi. Effect of scutellarein on acute pharyngitis in rats[J]. Journal of China Pharmaceutical University, 2019, 50(5): 600-605. DOI: 10.11665/j.issn.1000-5048.20190514
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野黄芩素对大鼠急性咽炎的治疗作用

  • 1.

    南京中医药大学第三临床医学院

  • 2.

    江苏省中医药研究院转化医学实验室

  • 3.

    中国药科大学天然药物化学教研室

Effect of scutellarein on acute pharyngitis in rats

摘要

    摘要
  • 摘要: 为了探究野黄芩素对急性咽炎的治疗作用，本研究将60只大鼠分为5组:空白组、急性咽炎模型组、野黄芩素低剂量组、野黄芩高剂量组和阳性药对照组，采用HE染色，血液细胞分析仪，IL-6、IL-1β、TNF-α ELISA试剂盒考察野黄芩素对急性咽炎大鼠体征状态，咽部组织形态，白细胞、中性粒细胞数目，血清IL-6、IL-1β、TNF-α浓度的影响。将40只小鼠分为4组:空白组、野黄芩低剂量组、野黄芩素高剂量组和阳性药对照组，采用小鼠热板实验和扭体实验考察野黄芩素的镇痛作用。实验结果发现:与模型组相比，2.5、10 mg/kg野黄芩素均可改善急性咽炎大鼠的体征状态和咽部组织形态，显著减少白细胞和中性粒细胞数目(P<；0.05，P<；0.01)，显著降低大鼠血清中TNF-α、IL-1β、IL-6水平(P<；0.01)；同时，10 mg/kg野黄芩素可以极显著提高热板实验中小鼠的痛阈值，并减少醋酸所致的扭体次数(P<；0.01)。实验结果表明，野黄芩素对大鼠急性咽炎具有潜在治疗作用，具有良好的治疗前景。
    Abstract: In order to investigate the therapeutic effects of scutellarein on acute pharyngitis, 60 rats were randomly divided into five groups: blank group, model group, low-dose scutellarein group, high-dose scutellarein group and positive drug group. HE staining, blood-cell-analyzer, IL-6, IL-1β and TNF-α ELISA kit were used to study the effects of scutellarein on acute pharyngitis in pharyngeal tissue morphology, the counts of white blood cells and neutrophil and the serum concentrations of TNF-α, IL-1β and IL-6. Meanwhile, forty mice were randomly divided into four groups: blank group, low-dose scutellarein group, high-dose scutellarein group and positive drug group. Then, hot plate and writhing test of mice were carried out to study the analgesic effects of scutellarein. Results showed that, compared to the model group, scutellarein improved the physical status of acute pharyngitis rats, reduced the number of white blood cells significantly(P< 0. 05)and decreased the number of neutrophils and the levels of TNF-α, IL-1β and IL-6 in rats serum significantly(P< 0. 01). Meanwhile, scutellarein dramatically improved the pain threshold in hot plate test and decreased the number of writhing mice(P< 0. 01). It can be concluded that scutellarein can be used to treat acute pharyngitis with its anti-inflammatory and analgesic effect.
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    • 参考文献(15)
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  • 刊出日期:  2019-10-24

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