• 中国中文核心期刊
  • 中国科学引文数据库核心期刊
  • 中国科技核心期刊
  • 中国高校百佳科技期刊
高级检索

基于TNF-α/MAPK/apoB-48通路研究首乌降脂汤对高脂血症金黄地鼠血脂的影响

娄彬, 王云杉, 谷明林, 王大为

娄彬, 王云杉, 谷明林, 王大为. 基于TNF-α/MAPK/apoB-48通路研究首乌降脂汤对高脂血症金黄地鼠血脂的影响[J]. 中国药科大学学报, 2019, 50(6): 728-733. DOI: 10.11665/j.issn.1000-5048.20190614
引用本文: 娄彬, 王云杉, 谷明林, 王大为. 基于TNF-α/MAPK/apoB-48通路研究首乌降脂汤对高脂血症金黄地鼠血脂的影响[J]. 中国药科大学学报, 2019, 50(6): 728-733. DOI: 10.11665/j.issn.1000-5048.20190614
shu
LOU Bin, WANG Yunshan, GU Minglin, WANG Dawei. Effect of Shouwu Jiangzhi decoction on hyperlipidemia Golden Syrian hamsters based on TNF-α/MAPK/apoB-48 pathway[J]. Journal of China Pharmaceutical University, 2019, 50(6): 728-733. DOI: 10.11665/j.issn.1000-5048.20190614
Citation: LOU Bin, WANG Yunshan, GU Minglin, WANG Dawei. Effect of Shouwu Jiangzhi decoction on hyperlipidemia Golden Syrian hamsters based on TNF-α/MAPK/apoB-48 pathway[J]. Journal of China Pharmaceutical University, 2019, 50(6): 728-733. DOI: 10.11665/j.issn.1000-5048.20190614
shu

基于TNF-α/MAPK/apoB-48通路研究首乌降脂汤对高脂血症金黄地鼠血脂的影响

  • 1.

    江苏省中医药研究院转化医学实验室

  • 2.

    中国药科大学中药学院中药制剂系&天然药物活性组分与药效国家重点实验室

基金项目: 江苏省中医药局科技项目基金资助(No.YB2017028)

Effect of Shouwu Jiangzhi decoction on hyperlipidemia Golden Syrian hamsters based on TNF-α/MAPK/apoB-48 pathway

摘要

    摘要
  • 摘要: 为探究首乌降脂汤抑制小肠分泌apoB-48治疗高脂血症的机制，将20只金黄地鼠按体重随机分为空白组、模型组、非诺贝特治疗组和首乌降脂汤组。高脂饲料喂养4周建立高血脂模型，连续灌胃给药4 周后，微板法检测血脂指标高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、甘油三酯(TG)、总胆固醇(TC)等的变化；酶联免疫法检测血清中肿瘤坏死因子-α(TNF-α)、载脂蛋白B-48(apoB-48)和游离脂肪酸(FFA)的水平；Western blot检测小肠组织中丝裂原活化蛋白激酶(MAPK)通路相关蛋白p38、ERK、JNK以及脂质代谢调节蛋白固醇原件蛋白(SREBP)、apoB-48的表达。实验结果显示首乌降脂汤可有效降低高血脂金黄地鼠血清中LDL-C、TG、FFA以及TNF-α和apoB-48的含量，显著下调小肠组织中JNK、p38、ERK、SREBP以及apoB-48的蛋白表达水平。上述结果提示首乌降脂汤可能通过抑制TNF-α /MAPK信号通路下调apoB-48的表达，发挥降血脂药效。
    Abstract: To investigate the mechanism of Shouwu Jiangzhi decoction in treatment of hyperlipidemia by suppress apoB-48 in small intestines, Golden Syrian hamsters were randomly devided into blank group, model group, fenobrate treatment group and Shouwu Jiangzhi decoction treatment group based on weight. The hyperlipidemia models of golden Syrian hamsters were induced by high fat diet(HFD)treatment for 4 weeks, then administered orally with drugs for 4 weeks. The serum indexes of HDL-C, LDL-C, TG and TC were determined by microplate methods, ELISA kits were used to evaluate the contents of serum TNF-α, apoB-48 and FFA. The protein expression levels of p38, ERK, JNK, SREBP, TNF-α and apoB-48 in small intestines were determined by Western blots. The results showed that Shouwu Jiangzhi decoction can effectively increase the serum HDL-C level and reduce the serum level of TG, LDL-C, TNF-α and apoB-48 in HFD-induced hamsters. Furthermore, Shouwu Jiangzhi decoction can significantly downregulate the protein expressions of p38, JNK, ERK, SREBP, TNF-α and apoB-48 in small intestines. Results above indicate that Shouwu Jiangzhi decoction may downregulate the protein expression of apoB-48 to treat hyperlipidemia via partially downregulating TNF-α/MAPK signal pathway.
    • HTML全文
    • 参考文献(19)
  • [1] Heine RJ.The role of postprandial hyperglycemia and hyperlipidemia in CVD risk[J].Curr Diab Rep,2006,6(1):1-2.
    [2] Hu DY.New guidelines and evidence for prevention and treatment of dyslipidemia and atherosclerotic cardiovascular disease in China[J].Chronic Dis Transl Med,2017,3(2):73-74.
    [3] Huang YX,Gao L,Xie XP,et al.Epidemiology of dyslipidemia in Chinese adults:meta-analysis of prevalence,awareness,treatment,and control[J].Popul Health Metr,2014,12(1):28.
    [4] Lindman AS,Veierød MB,Tverdal A,et al.Nonfasting triglycerides and risk of cardiovascular death in men and women from the norwegian counties study[J].Eur J Epidemiol,2010,25(11):789-798.
    [5] Nordestgaard BG,Benn M,Schnohr P,et al.Nonfasting triglycerides and risk of myocardial infarction,ischemic heart disease,and death in men and women[J].JAMA,2007,298(3):299.
    [6] Nakajima K,Nagamine T,Fujita MQ,et al.Apolipoprotein B-48:a unique marker of chylomicron metabolism[J].Adv Clin Chem,2014,64:117-177.
    [7] Qin BL,Qiu W,Avramoglu RK,et al.Tumor necrosis factor-alpha induces intestinal insulin resistance and stimulates the overproduction of intestinal apolipoprotein B48-containing lipoproteins[J].Diabetes,2007,56(2):450-461.
    [8] van Breukelen-van der Stoep D,van Zeben D,de Vries M,et al.Plasma apolipoprotein B48 levels in patients with rheumatoid arthritis:evaluating novel cardiovascular risk factors[J].Arthritis Rheumatol,2015,67:2.
    [9] Federico LM,Naples M,Taylor D,et al.Intestinal insulin resistance and aberrant production of apolipoprotein B48 lipoproteins in an animal model of insulin resistance and metabolic dyslipidemia:evidence for activation of protein tyrosine phosphatase-1B,extracellular signal-related kinase,and sterol regulatory element-binding protein-1c in the fructose-fed hamster intestine[J].Diabetes,2006,55(5):1316-1326.
    [10] Lou B,Wang DC,Xu CB,et al.Clinical efficacy of Shouwu Jiangzhi tablets in treating hyperlipemia[J].Chin J Tradit Chin Med Pharm(中华中医药学刊),2007,25(2):315-316.
    [11] Lou DX,Yan D,Guo M,et al.Antipyretic effect of isodon eriocalyx extracts on yeast-induced fever in rats [J].J China Pharm Univ(中国药科大学学报),2019,50(1):87-92.
    [12] Eberlé D,Hegarty B,Bossard P,et al.SREBP transcription factors:master regulators of lipid homeostasis[J].Biochimie,2004,86(11):839-848.
    [13] Yoshimura N,Kinoshita M,Teramoto T.Isolation and characterization of apolipoprotein B48-containing lipoproteins with a monoclonal antibody against apolipoprotein B48[J].J Atheroscler Thromb,2009,16(6):740-747.
    [14] Choi RY,Lee HI,Ham JR,et al.Heshouwu(Polygonum multiflorum Thunb.)ethanol extract suppresses pre-adipocytes differentiation in 3T3-L1 cells and adiposity in obese mice[J].Biomedecine Pharmacother,2018,106:355-362.
    [15] Zhu HF,Wang Y,Liu ZQ,et al.Antidiabetic and antioxidant effects of catalpol extracted from Rehmannia glutinosa(Di Huang)on rat diabetes induced by streptozotocin and high-fat,high-sugar feed[J].Chin Med,2016,11:25.
    [16] Kwok CY,Wong CNY,Yau MYC,et al.Consumption of dried fruit of Crataegus pinnatifida(hawthorn)suppresses high-cholesterol diet-induced hypercholesterolemia in rats[J].J Funct Foods,2010,2(3):179-186.
    [17] Wang T,An YT,Zhao CF,et al.Regulation effects of Crataegus pinnatifida leaf on glucose and lipids metabolism[J].J Agric Food Chem,2011,59(9):4987-4994.
    [18] Shui YM,Lv GY,Shan LT,et al.Epimedin C promotes vascularization during BMP2-induced osteogenesis and tumor-associated angiogenesis[J].Am J Chin Med,2017,45(5):1093-1111.
    [19] Li XY,Peng BJ,Pan YB,et al.Icariin stimulates osteogenic differentiation and suppresses adipogenic differentiation of rBMSCs via estrogen receptor signaling[J].Mol Med Rep,2018,18(3):3483-3489.
    • 相关文章
    • 施引文献(9)

  • 期刊类型引用(2)

    1. 曹雷，刘明军，吴春炜，陈海龙，王若琳，包扬，何泽. 中药调控MAPK通路治疗肥胖的研究进展. 中国实验方剂学杂志. 2024(09): 254-262 . 百度学术
    2. 王辉敏，李冠文，杨金梅，陈超，刘星，秦楠. 多不饱和脂肪酸降脂作用机制的研究进展. 中国油脂. 2023(05): 73-77+84 . 百度学术

    其他类型引用(7)

    • 资源附件(0)
计量
  • 文章访问数:  355
  • HTML全文浏览量:  5
  • PDF下载量:  635
  • 被引次数: 9
出版历程
  • 刊出日期:  2019-12-24

目录

摘要
HTML全文
    参考文献(19)
    相关文章
    施引文献(9)
    资源附件(0)

    /

    返回文章
    返回

    版权所有：《中国药科大学学报》编辑部苏ICP备05007142号

    地址：江苏省南京市鼓楼区童家巷24号（210009）电话: 025-83271566E-mail: xuebao@cpu.edu.cn

    本系统由 北京仁和汇智信息技术有限公司 开发  百度统计

    访问量：336173

    x 关闭 永久关闭