金水宝片对顺铂所致大鼠急性肾损伤的保护作用及机制
Protective effect and mechanism of Jinshuibao tablet on acute kidney injury induced by cisplatin in rats
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摘要: 考察金水宝片对顺铂诱导大鼠急性肾损伤的防治作用及机制。分别采用单次腹腔注射顺铂5 mg/kg和连续5 d静脉注射顺铂2 mg/kg诱导大鼠急性肾损伤模型。观察大鼠急性肾损伤模型分别经预防性或治疗性给予金水宝片后的肾功能的变化,给药结束后进行肾脏组织病理学检查,并检测肾脏肿瘤坏死因子-α(TNF-α)和活性氧(ROS)的含量、半胱天冬酶3(Caspase 3)活性以及t-p38、p-p38、Bax及Bcl-2的蛋白表达。结果显示,金水宝片预防性给药和治疗性给药均能显著抑制顺铂诱导的大鼠急性肾损伤模型的血清尿素氮(BUN)和肌酐(CRE)水平的上升,显著增加肾脏肌酐清除率,显著改善肾小管变性和炎症浸润,且预防性给药的起效时间较治疗性给药缩短。同时两种给药方式均能显著降低肾脏TNF-α、ROS含量以及Caspase 3活性。Western blot结果显示,金水宝片能显著减少肾脏中p-p38、t-p38的蛋白表达,显著增大Bcl-2/Bax比值。研究结果表明,金水宝预防性给药和治疗性给药均能通过改善肾功能和肾脏组织病理变化,以达到对顺铂所致急性肾损伤的防治作用,且其作用机制可能与金水宝抑制TNF-α,抑制ROS-p38MAPK-Caspase 3通路,进而抑制细胞凋亡有关。Abstract: This study aimed to observe the therapeutic effect and mechanism of Jinshuibao tablet on acute renal injury induced by cisplatin. Acute renal injury models in SD rats were induced separately by single intraperitoneal injection of cisplatin(5 mg/kg)and intravenous injection for 5 consecutive days at a dosage of 2 mg/kg per day. The renal function and renal histopathological changes were observed in rat acute renal injury models after prevention and treatment with Jinshuibao tablet, respectively. The content of tumor necrosis factor(TNF-α)and reactive oxygen species(ROS), the activity of Caspase 3 and the expression of t-p38, p-p38, Bax and Bcl-2 in the kidneys were detected. The results showed that preventive and therapeutic administration of Jinshuibao tablets could both significantly inhibit the increase of the blood urea nitrogen(BUN)and creatinine(CRE), increase the creatinine clearance rate, reduce the contents of TNF-α and ROS, and decrease the activity of Caspase 3 in acute renal injury models induced by cisplatin. The renal histopathological results showed that Jinshuibao tablets could significantly reduce renal histopathology scores, ameliorate renal tubule degeneration and inflammatory infiltration. Western blot results showed that Jinshuibao tablets could significantly decrease the expression of t-p38 and p-p38, while increasing the Bcl-2/Bax ratio in the kidneys. These results suggested that preventive and therapeutic administration of Jinshuibao tablets could both improve renal function and pathological changes of renal tissue, which might be related to the inhibition of TNF-α and the ROS-p38 MAPK-Caspase3 pathway and thus inhibition of apoptosis.
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Keywords:
- Jinshuibao tablets /
- cisplatin /
- acute kidney injury /
- mechanism
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[1] Chen CJ,Chen CN,Pan QZ,et al.Clinical study on the intervention therapy of Jinshuibao capsule on cisplatins-induced renal toxicity[J].Med Innov China(中国医学创新),2012,9(2):32-33. [2] Ye XL,Zhen XC,Yang XL,et al.Diclofenac sodium associated acute kidney injury[J].J ADR(药物不良反应杂志),2018,20(2):146-147. [3] Liu W,Jiang SF,Tu L,et al.Mechanism of JinShuiBao capsule in ameliorating rat pneumoconiosis model[J].J China Pharm Univ(中国药科大学学报),2018,49(4):476-482. [4] Yan YL,Zhang DM,Wang Y,et al.The comparative study of the effect of intraperitoneal perfusion and intravenous chemotherapy in advanced ovarian cancer[J].J Int Obstet Gynecol(国际妇产科学杂志),2014,41(6):655-657. [5] Markman M.Intraperitoneal chemotherapy as primary treatment of advanced ovarian cancer:efficacy,toxicity,and future directions[J].Rev Recent Clin Trials,2007,2(3):169-173. [6] Goren O,Matot I.Update on perioperative acute kidney injury[J].Curr Opin Crit Care,2016,22(4):370-378. [7] Yan W, Xu Y, Yuan YH, et al. Renoprotective mechanisms of Astragaloside IV in cisplatin-induced acute kidney injury[J].Free Radic Res,2017,51(7/8):669-683. [8] Mulay SR,Anders HJ.Crystal nephropathies:mechanisms of crystal-induced kidney injury[J].Nat Rev Nephrol,2017,13(4):226-240. [9] Devarajan P.Acute kidney injury:still misunderstood and misdiagnosed[J].Nat Rev Nephrol,2017,13(3):137-138. [10] Mansouri A,Ridgway LD,Korapati AL,et al.Sustained activation of JNK/p38 MAPK pathways in response to cisplatin leads to Fas ligand induction and cell death in ovarian carcinoma cells[J].J Biol Chem,2003,278(21):19245-19256. [11] He B,Tao HY,Liu SQ,et al.Carboxymethylated chitosan protects rat chondrocytes from NO-induced apoptosis via inhibition of the p38/MAPK signaling pathway[J].Mol Med Rep,2016,13(3):2151-2158. [12] Wang JX,Chen HJ,Cao P,et al.Inflammatory cytokines induce caveolin-1/β-catenin signalling in rat nucleus pulposus cell apoptosis through the p38 MAPK pathway[J].Cell Prolif,2016,49(3):362-372. [13] Cai QH,Tang Y,Fan SH,et al.In vivo effects of dexmedetomidine on immune function and tumor growth in rats with ovarian cancer through inhibiting the p38MAPK/NF-κB signaling pathway[J].Biomedecine Pharmacother,2017,95:1830-1837. [14] Goldsmith EJ,Min XS,He HX,et al.Structural studies of MAP kinase cascade components[J].Methods Mol Biol,2010,661:223-237. [15] Sabio G,Davis RJ.TNF and MAP kinase signalling pathways[J].Semin Immunol,2014,26(3):237-245. [16] Ashraf M,Ebner M,Wallner C,et al.A p38MAPK/MK2 signaling pathway leading to redox stress,cell death and ischemia/reperfusion injury[J].Cell Commun Signal,2014,12(1):6.
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