新疆一枝蒿药材双波长HPLC指纹图谱的研究

张逸凡; 鲍凤霞; 夏振江; 尚靖

doi:10.11665/j.issn.1000-5048.20200207

新疆一枝蒿药材双波长HPLC指纹图谱的研究

1.
中国药科大学中药学院
2.
南京大学环境学院，污染控制与资源化研究国家重点实验室

基金项目: 国家自然科学基金青年科学基金项目(No.41907365)；青海省藏药药理学和安全性评价研究重点实验室开放课题基金(No.2019-ZY-02)

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出版历程
- 刊出日期: 2020-04-24

HPLC dual-wavelength fingerprint of Artemisia rupestris L.

摘要

摘要: 建立新疆一枝蒿药材双波长HPLC指纹图谱的测定方法，为药材质量标准提升提供科学依据。采用HPLC法，Zorbax SB-C₁₈色谱柱(4.6 mm×250 mm，5 μm)，柱温32 ℃；流动相:甲醇-0.2%甲酸水梯度洗脱；流速1.0 mL/min；检测器:DAD检测波长245和325 nm；进样量20 μL。建立了新疆一枝蒿的HPLC指纹图谱，分别在245和325 nm处确定了7个和8个共有峰，并确证了槲皮素、绿原酸、木犀草素、芹菜素、蔓荆子素5个色谱峰，9批次新疆一枝蒿药材与生成对照指纹图谱的相似度大于0.9。首次建立了新疆一枝蒿药材双波长HPLC指纹图谱，所建立的指纹图谱测定方法精密度、稳定性和重复性良好，可用于新疆一枝蒿药材的鉴别和质量控制。
- 新疆一枝蒿 /
- HPLC /
- 双波长 /
- 指纹图谱 /
- 质量控制
Abstract: To establish HPLC dual-wavelength fingerprint of Artemisia rupestris L. , and provide a scientific basis for the improvement of its quality specifications. The separation was performed on an Agilent Zorbax SB-C₁₈(4. 6 mm×250 mm, 5 μm)column maintained at 32 ℃, with methanol-0. 2% formic acid-water gradient elution at flow rate of 1. 0 mL/min and the UV detection wavelength at both 245 and 325 nm. The sample injection volume was 20 μL. The fingerprint of Artemisia rupestris L. was established. 7 and 8 common peaks were found, respectively, of which 5 common peaks were identified, and the similarity among 9 batches of Artemisia rupestris L. and the fingerprints of control was over 0. 9. The HPLC dual-wavelength fingerprint of Artemisia rupestris L. was established for the first time, providing a new scientific basis for its identification and quality control.
- Artemisia rupestris L. /
- HPLC /
- dual-wavelength /
- fingerprint /
- quality control

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参考文献(11)

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