雷公藤红素联合用药对HepG2细胞增殖的抑制作用

张雪; 欧阳惠枝; 徐伟; 王晓颖

doi:10.11665/j.issn.1000-5048.20200209

雷公藤红素联合用药对HepG2细胞增殖的抑制作用

福建中医药大学药学院

基金项目: 国家自然科学基金资助项目(No.81603301)；福建省卫计委中青年骨干人才培养项目资助(No.2016-ZQN-69)；福建中医药大学科研平台校管课题资助项目(X2019006-平台)

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出版历程
- 刊出日期: 2020-04-24

Effect of celastrol combined medication on the inhibition of HepG2 cell proliferation

摘要

摘要: 研究雷公藤红素分别与甘草次酸、紫杉醇、大黄酸联合用药对人肝癌HepG2细胞增殖的抑制作用。采用MTT法，测定并计算雷公藤红素分别与甘草次酸、紫杉醇、大黄酸联用后HepG2细胞的存活率，利用协同指数(CI)、金氏公式判定协同药效。同时检测细胞凋亡情况以及细胞周期阻滞情况。结果表明，单独应用雷公藤红素、甘草次酸、大黄酸、紫杉醇均可抑制HepG2细胞增殖；雷公藤红素分别与甘草次酸、紫杉醇、大黄酸联用，能显著增强雷公藤红素对HepG2细胞增殖抑制效果，联合用药在一定浓度范围内呈现良好协同作用；联合用药使HepG2细胞凋亡率显著提高(P<；0.01)，并使更多HepG2细胞阻滞于G₂/M期。雷公藤红素与甘草次酸、紫杉醇、大黄酸联用对HepG2细胞的增殖具有良好的协同抑制效果，其与共同诱导凋亡及增加细胞G₂/M期阻滞相关。
- 雷公藤红素 /
- 大黄酸 /
- 紫杉醇 /
- 甘草次酸 /
- HepG2细胞 /
- 联合用药 /
- 协同作用
Abstract: To study the inhibitory effect of celastrol respectively combined with glycyrrhetic acid, paclitaxel, and rhein on the proliferation of human hepatoma cell line HepG2. The MTT method was used to detect the survival rate of HepG2 cells. The cooperativity index(CI)and Jin′s formula method were used to determine the synergistic effect. Apoptosis and cell cycle arrest were detected, too. The results show that celastrol, glycyrrhetinic acid, rhein, and paclitaxel alone can inhibit the proliferation of HepG2 cells, respectively. Combination with glycyrrhetic acid, paclitaxel, and rhein, respectively, the inhibitory effect of celastrol on the proliferation of HepG2 cells was significantly enhanced. And the synergistic effect on the proliferation inhibition of HepG2 cells in some concentrations was displayed in the experiment. The cell apoptosis rate was improved(P< 0. 01)and more cells were arrested in G₂/M phase. Celastrol respectively combined with glycyrrhetic acid, paclitaxel, and rhein displayed a synergistic inhibitory effect on the proliferation of HepG2 cells, and the effect was related to inducing cell apoptosis and increasing the cell cycle arrest in G₂/M phase.
- celastrol /
- rhein /
- paclitaxel /
- glycyrrhetic acid /
- HepG2 cells /
- combined medication /
- synergy effect

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参考文献(21)

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