黄芪对小鼠心脑血管系统代谢稳态的影响

邓小颖; 杨旭萍; 刘佩芳; 张尊建; 黄寅

doi:10.11665/j.issn.1000-5048.20200416

黄芪对小鼠心脑血管系统代谢稳态的影响

1.
中国药科大学药物质量与安全预警教育部重点实验室，南京 210009
2.
哈尔滨医科大学附属第二医院神经内科，哈尔滨 150086

基金项目: 黑龙江省自然科学基金资助项目（No.QC2016109)；药物质量与安全预警教育部重点实验室开放课题资助项目（No.DQCP2017MS02）

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出版历程
- 收稿日期: 2020-05-25
- 刊出日期: 2020-08-24

Effect of Astragalus membranaceus on metabolic homeostasis of cardio-cerebrovascular system in mice

1.
Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), China Pharmaceutical University, Nanjing 210009
2.
Department of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086,China

Funds: This study was Foundation:supported by the Natural Science Foundation of Heilongjiang Province (No.QC2016109) and the Open Program of MOE Key Laboratory of Drug Quality Control and Pharmacovigilance (No.DQCP2017MS02)

摘要

摘要: 研究中药黄芪对小鼠心脏、大脑及血液代谢稳态的影响，从代谢调控角度阐明黄芪“修心健脑”的作用机制。13只ICR雄性小鼠随机分为两组，分别连续10 d灌胃给予超纯水和黄芪水提液，采用液相色谱-质谱（LC-MS）和气相色谱-质谱（GC-MS）联用分析全面表征小鼠血清、心脏和脑组织代谢指纹图谱，结合多元统计分析与非参数检验筛选、鉴定差异代谢物，聚焦相关代谢通路。多元统计分析表明，与对照组相比，黄芪干预后小鼠机体的代谢轮廓变化显著，在血清、心脏、脑中分别鉴定出15、19和17个差异代谢物，其中差异代谢物棕榈酸和LysoPC(20∶3)为3种生物样本共有且变化趋势一致。代谢通路富集分析结果显示，氨基酸代谢、磷脂代谢、脂肪酸代谢和三羧酸循环等通路受到显著影响。研究结果显示，黄芪可能通过调节能量代谢、氨基酸代谢及脂代谢稳态发挥心脑血管保护作用。
- 黄芪 /
- 心脑血管疾病 /
- 代谢组学 /
- 质谱
Abstract: To explore the effect of Astragalus membranaceus, a traditional Chinese medicine, on metabolic homeostasis of heart, brain and blood in mice, and to elucidate the cardio-cerebrovascular protective mechanisms of AR from the perspective of metabolic regulation. Thirteen ICR male mice were randomly divided into two groups which were intragastrically administered with ultrapure water and aqueous extract of Astragalus membranaceus for 10 consecutive days, respectively. Liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS) were used to comprehensively characterize the metabolic profiles of serum, heart and brain tissues. Multivariate statistical analysis combined with nonparametric tests were applied to screen and identify different metabolites, and then the related metabolic pathways were uncovered. Multivariate statistical analysis showed that the metabolic profiles of serum, heart, and brain tissues of mice after Astragalus membranaceus intervention significantly changed compared with the control group. A total of 15, 19, and 17 metabolites were identified in serum, heart, and brain tissues, respectively, among which palmitic acid and LysoPC (20∶3) were screened out from all types of biological samples. The results of metabolic pathway enrichment analysis showed that amino acid metabolism, phospholipid metabolism, fatty acid metabolism and tricarboxylic acid cycle were significantly affected. Astragalus membranaceus may protect the cardio-cerebrovascular system by regulating the metabolic homeostasis of amino acids, lipids and energy.
- Astragalus membranaceus /
- cardio-cerebrovascular disease /
- metabolomics /
- mass spectrometry

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