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用于新型冠状病毒核酸检测的即时检测方法研究进展

鲍瑶菲, 薛茜茹, 武海萍, 邹秉杰, 宋沁馨, 周国华

鲍瑶菲, 薛茜茹, 武海萍, 邹秉杰, 宋沁馨, 周国华. 用于新型冠状病毒核酸检测的即时检测方法研究进展[J]. 中国药科大学学报, 2020, 51(6): 635-645. DOI: 10.11665/j.issn.1000-5048.20200601
引用本文: 鲍瑶菲, 薛茜茹, 武海萍, 邹秉杰, 宋沁馨, 周国华. 用于新型冠状病毒核酸检测的即时检测方法研究进展[J]. 中国药科大学学报, 2020, 51(6): 635-645. DOI: 10.11665/j.issn.1000-5048.20200601
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BAO Yaofei, XUE Qianru, WU Haiping, ZOU Bingjie, SONG Qinxin, ZHOU Guohua. Advances in point-of-care testing for new corona virus nucleic acid[J]. Journal of China Pharmaceutical University, 2020, 51(6): 635-645. DOI: 10.11665/j.issn.1000-5048.20200601
Citation: BAO Yaofei, XUE Qianru, WU Haiping, ZOU Bingjie, SONG Qinxin, ZHOU Guohua. Advances in point-of-care testing for new corona virus nucleic acid[J]. Journal of China Pharmaceutical University, 2020, 51(6): 635-645. DOI: 10.11665/j.issn.1000-5048.20200601
shu

用于新型冠状病毒核酸检测的即时检测方法研究进展

  • 1.

    中国药科大学药物质量与安全预警教育部重点实验室，南京 210009

  • 2.

    东部战区总医院临床药学科，南京 210002

  • 3.

    南方医科大学药学院，广州 510515

基金项目: 国家自然科学基金资助项目（No.81673390）；江苏省自然科学基金资助项目（No.BK20191322）；江苏省杰出青年基金资助项目（No.BK20180005）；大学生创新创业训练计划项目资助（No.202010316032S）

Advances in point-of-care testing for new corona virus nucleic acid

Funds: This study was supported by the National Natural Science Foundation of China (No.81673390), the Natural Science Foundation of Jiangsu Province (No. BK20191322),the Jiangsu Provincial Science Fund for Distinguished Young Scholars (No.BK20180005) and the Undergraduate Training Programs for Innovation and Entrepreneurship(No.202010316032S)

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    摘要
  • 摘要: 新型冠状病毒（SARS-CoV-2）引起的新型冠状病毒肺炎（corona virus disease 2019，COVID-19）在全世界范围内快速传播，截至2020年9月30日全球感染人数已达3 300万人，造成100多万人死亡，给公众的生命健康造成严重威胁。基于实验室的标准化核酸检测方法周转时间长、成本高，因此急需开发便捷检测新冠病毒的方法，实现在资源有限的情况下快速测试以及时控制疫情。本文从提取、扩增、检测3个方面总结了针对新型冠状病毒所开发的核酸即时检测方法（point-of-care testing，POCT），并对整合了这3个步骤的商业化即时检测装置进行简要介绍，以期为COVID-19及其他新兴传染病的紧急应对和快速部署提供参考。
    Abstract: The corona virus disease 2019 (COVID-19) caused by the new coronavirus (SARS-CoV-2) has spread rapidly around the world,posing a serious threat to the public"s health. As of September 30,2020,the number of infected people in the world has reached 33 million,causing more than 1 million deaths. Normalized nucleic acid detection methods based on lab have long turnaround time and high cost. Therefore,there is an urgent need to develop a convenient method to detect SARS-CoV-2,so as to achieve rapid testing and timely control of the epidemic when resources are limited.This review summarizes the point-of-care testing (POCT) methods developed for SARS-CoV-2 in terms of extraction,amplification and detection,and briefly introduces commercial POCT instruments that integrate these three steps,in order to provide references for emergency response and rapid deployment of COVID-19 and other emerging infectious diseases.
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  • 收稿日期:  2020-09-16
  • 修回日期:  2020-11-08
  • 刊出日期:  2020-12-24

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