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热敏型可修复分子印迹固相微萃取纤维的制备及其应用

郭跃龙, 吴丹, 郑枫, 纪顺利

郭跃龙, 吴丹, 郑枫, 纪顺利. 热敏型可修复分子印迹固相微萃取纤维的制备及其应用[J]. 中国药科大学学报, 2020, 51(6): 702-710. DOI: 10.11665/j.issn.1000-5048.20200609
引用本文: 郭跃龙, 吴丹, 郑枫, 纪顺利. 热敏型可修复分子印迹固相微萃取纤维的制备及其应用[J]. 中国药科大学学报, 2020, 51(6): 702-710. DOI: 10.11665/j.issn.1000-5048.20200609
GUO Yuelong, WU Dan, ZHENG Feng, JI Shunli. Preparation and application of thermosensitive and repairable molecularly imprinted solid phase microextraction fiber[J]. Journal of China Pharmaceutical University, 2020, 51(6): 702-710. DOI: 10.11665/j.issn.1000-5048.20200609
Citation: GUO Yuelong, WU Dan, ZHENG Feng, JI Shunli. Preparation and application of thermosensitive and repairable molecularly imprinted solid phase microextraction fiber[J]. Journal of China Pharmaceutical University, 2020, 51(6): 702-710. DOI: 10.11665/j.issn.1000-5048.20200609

热敏型可修复分子印迹固相微萃取纤维的制备及其应用

基金项目: 国家自然科学基金资助项目(No.81703472);中央高校基本科研业务费专项资金资助项目(No.2632020ZD05)

Preparation and application of thermosensitive and repairable molecularly imprinted solid phase microextraction fiber

Funds: This study was supported by the National Natural Science Foundation of China (No.81703472),and the Fundamental Research Funds for the Central Universities (No.2632020ZD05)
  • 摘要: 以螺旋霉素为模板分子,甲基丙烯酸和N-异丙基丙烯酰胺为功能单体,乙二醇甲基丙烯酸酯为交联剂,硅烷化的石英毛细管为载体合成了具有热敏型、可修复的分子印迹固相微萃取纤维。通过扫描电镜和氮气吸附/脱附对制备的分子印迹固相微萃取纤维进行表征,并对影响萃取效果的参数进行了优化。分子印迹固相微萃取纤维对大环内酯类抗生素具有高选择性和灵敏性,与高效液相色谱联用可对食品基质中的螺旋霉素、替米考星、泰乐菌素、交沙霉素4种大环内酯类抗生素进行定量分析,在0.5~50 μg/mL范围内,色谱峰面积与浓度呈良好的线性关系,样品在3种不同添加水平下的加标回收率在81.8%~119.1%之间,日间精密度均小于13.8%(n=6),日内精密度均小于15.5%(n=3)。
    Abstract: In this study,thermosensitive and repairable molecularly imprinted solid-phase microextraction fibers were synthesized using spiramycin as template molecule,methacrylic acid and N-isopropylacrylamide as functional monomers,ethylene glycol dimethacrylate as crosslinking agent,and silanized quartz capillary as carrier. The prepared molecularly imprinted solid-phase microextraction fibers were characterized by scanning electron microscope and nitrogen adsorption/desorption,and various parameters affecting the extraction efficiency were optimized. Due to high selectivity and sensitivity of the fibers for macrolide antibiotics,the quantitative analysis of four macrolide antibiotics in food matrix,spiramycin,tilmicosin,tylosin,and josamycin,was peroformed in combination with high performance liquid chromatography. In the range of 0.5 to 50 μg/mL,the chromatographic peak area showed a good linear relationship with the concentration. The spike recoveries of the samples at three different addition levels were between 81.8% and 119.1%;the inter-day precisions were less than 13.8% (n=6),and the intra-day precisions were less than 15.5% (n=3).
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出版历程
  • 收稿日期:  2020-04-22
  • 修回日期:  2020-09-16
  • 刊出日期:  2020-12-24

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