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艾叶乙醇提取物对糖尿病小鼠血糖和血脂的影响

肖建琪, 徐健, 束方荣, 胡晓芬, 柳文媛, 冯锋

肖建琪, 徐健, 束方荣, 胡晓芬, 柳文媛, 冯锋. 艾叶乙醇提取物对糖尿病小鼠血糖和血脂的影响[J]. 中国药科大学学报, 2021, 52(1): 71-76. DOI: 10.11665/j.issn.1000-5048.20210110
引用本文: 肖建琪, 徐健, 束方荣, 胡晓芬, 柳文媛, 冯锋. 艾叶乙醇提取物对糖尿病小鼠血糖和血脂的影响[J]. 中国药科大学学报, 2021, 52(1): 71-76. DOI: 10.11665/j.issn.1000-5048.20210110
XIAO Jianqi, XU Jian, SHU Fangrong, HU Xiaofen, LIU Wenyuan, FENG Feng. Effect of Artemisia Argyi Folium ethanolic extract on blood glucose and blood lipids in diabetic mice[J]. Journal of China Pharmaceutical University, 2021, 52(1): 71-76. DOI: 10.11665/j.issn.1000-5048.20210110
Citation: XIAO Jianqi, XU Jian, SHU Fangrong, HU Xiaofen, LIU Wenyuan, FENG Feng. Effect of Artemisia Argyi Folium ethanolic extract on blood glucose and blood lipids in diabetic mice[J]. Journal of China Pharmaceutical University, 2021, 52(1): 71-76. DOI: 10.11665/j.issn.1000-5048.20210110

艾叶乙醇提取物对糖尿病小鼠血糖和血脂的影响

基金项目: 江苏省淮安市自然科学基金资助项目(No.HAB202066);江苏食品药品职业技术学院面上引导基金资助项目(No.JSFP2019003, No.JSFP2019009)

Effect of Artemisia Argyi Folium ethanolic extract on blood glucose and blood lipids in diabetic mice

Funds: The study was supported by the Natural Science Foundation of Huai"an of Jiangsu Province (No.HAB202066) and the General Program Foundation of Jiangsu Food and Pharmaceutical Science College (No.JSFP2019003, No.JSFP2019009)
  • 摘要: 为评价艾叶乙醇提取物对高脂饮食联合链脲佐菌素(STZ)诱导的糖尿病模型小鼠血糖血脂的影响,采用腹腔注射STZ(35 mg/kg)加高糖高脂饲料喂养ICR小鼠,建立糖尿病小鼠模型。糖尿病小鼠随机分成3组,模型组(5 mL/kg 0.5% CMC-Na)、艾叶乙醇提取物低剂量组(100 mg/kg)和高剂量组(400 mg/kg)。连续给药6周,每天记录小鼠的饮水量及摄食量,每周记录并比较小鼠的血糖指标。测定给药6周后小鼠血清总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密脂蛋白胆固醇(LDL-C)和糖耐量(OGTT)水平。结果显示,艾叶乙醇提取物高剂量组小鼠的饮水量、摄食量、体重及空腹血糖和血糖浓度-时间曲线下面积(AUC)明显降低(P < 0.01),口服葡萄糖耐量也显著改善(P < 0.01),艾叶乙醇提取物低剂量组小鼠的TG、TC和LDL-C显著降低(P < 0.01)。实验结果提示艾叶乙醇提取物对糖尿病小鼠糖脂代谢具有显著调节、改善作用,且呈现一定的剂量依赖性。
    Abstract: To investigate the effect of ethanolic extract from Artemisia Argyi Folium on blood glucose and blood lipids in diabetic mice, ICR mice were induced by intraperitoneal injection of 35 mg/kg streptozotocin (STZ) and a high-carbohydrate/high-fat diet to construct type 2 diabetes mellitus model. Diabetic mice were randomly divided into three groups: the model group (5 mL/kg 0.5% CMC-Na), the Artemisia Argyi Folium ethanolic extract low-dose group (100 mg/kg ) and high-dose group (400 mg/kg ). During the treatment for 6 weeks, the amount of drinking water and food intake of mice were recorded every day. Blood glucose and body weight were recorded every week. After treatment for 6 weeks, serum total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C),and oral glucose tolerance (OGTT) were measured. The results showed that the amount of drinking water and food intake of mice significantly decreased (P < 0.01) in the Artemisia Argyi Folium ethanolic extract high-dose group; oral glucose tolerance was significantly improved (P < 0.01) and the contents of TC, TG and LDL-C were significantly decreased in the Artemisia Argyi Folium ethanolic extract low-dose group (P < 0.01). The ethanolic extract from Artemisia Argyi Folium could significantly improve the glucose and lipid metabolic disorder in T2DM mice in a dose-dependent manner.
  • [1] . J Agric Food Chem, 2017, 65(38): 8319-8330.
    [2] Takeda C, Takeuchi M, Kawasaki Y, et al. Prophylactic sivelestat for esophagectomy and in-hospital mortality: a propensity score-matched analysis of claims database[J]. J Anesth, 2019, 33(2): 230-237.
    [3] Li XZ, Xu AJ, Sheng HY, et al. Early transition from insulin to sulfonylureas in neonatal diabetes and follow-up: experience from China[J]. Pediatr Diabetes, 2018, 19(2): 251-258.
    [4] Adams AS, Schmittdiel JA, Altschuler A, et al. Automated symptom and treatment side effect monitoring for improved quality of life among adults with diabetic peripheral neuropathy in primary care: a pragmatic, cluster, randomized, controlled trial[J]. Diabet Med, 2019, 36(1): 52-61.
    [5] Han BS, Xin ZQ, Ma SS, et al. Comprehensive characterization and identification of antioxidants in Folium Artemisiae Argyi using high-resolution tandem mass spectrometry[J]. J Chromatogr B Analyt Technol Biomed Life Sci, 2017, 1063: 84-92.
    [6] Li ZZ, Lv JL, Zhang LB, et al. Chemical constituents and pharmacology activities of Artemisia argyi:research advances[J]. J Int Pharm Res(国际药学研究杂志), 2016, 43(6): 1059-1066.
    [7] Ge YB, Wang ZG, Xiong Y, et al. Anti-inflammatory and blood stasis activities of essential oil extracted from Artemisia argyi leaf in animals[J]. J Nat Med, 2016, 70(3): 531-538.
    [8] Lan MB, Zhang YH, Zheng Y, et al. Antioxidant and immunomodulatory activities of polysaccharides from moxa (Artemisia argyi) leaf[J]. Food Sci Biotechnol, 2010, 19(6): 1463-1469.
    [9] Lv JL, Li ZZ, Zhang LB. Two new flavonoids from Artemisia argyi with their anticoagulation activities[J]. Nat Prod Res, 2018, 32(6): 632-639.
    [10] Xiao JQ, Liu WY, Sun HP, et al. Bioactivity-based analysis and chemical characterization of hypoglycemic and antioxidant components from Artemisia argyi[J]. Bioorg Chem, 2019, 92: 103268.
    [11] Chen XM, Tan SM, Huang Y, et al. Hypoglycemic effect of Rosa roxburghii, Morus alba and Momordica charantia beverage on diabetic mice[J]. China Brewing(中国酿造), 2019,38(6):123-127.
    [12] Tian YL, Zhang Y, Halemahebai G, et al. Urolithin A activates autophagy to improve liver insulin resistance in diabetic mice[J]. Chin Tradit Herbal Drugs(中草药), 2020, 51(3): 710-716.
    [13] Song X, Wang TT, Yang XD, et al. Purification and hypoglycemic effect of Eriobotrya japonica leaves extract containing corosolic acid[J]. Chin J New Drugs(中国新药杂志), 2017,26(2): 214-219.
    [14] Chen YH, Zhang J, Ai Zhipeng, et al. Effects of Chrysanthemum indicum extract on hyperglycemia, hyperlipidemia and aldose reductase in KKAy diabetic mice[J]. Chin J Pharmacol Toxicol(中国药理学与毒理学杂志), 2016,30(11): 1142-1148.
    [15] Xu BQ, Dai YQ, Fu QY, et al. Effects of eucommia flavonoids on Nrf2 / HO-1 oxidative stress signaling pathway in diabetic nephropathy mice[J]. Jilin J Chin Med(吉林中医药), 2020, 40(6): 788-791.
    [16] Duan ZF, Chen MD, Wang ZY. Yishenkang pills can downregulate fasting blood glucose and insulin in rats with insulin resistance[J]. J New Chin Med(新中医), 2017, 49(12): 1-3.
    [17] Gheibi S, Kashfi K, Ghasemi A. A practical guide for induction of type-2 diabetes in rat: incorporating a high-fat diet and streptozotocin[J]. Biomedecine Pharmacother, 2017, 95: 605-613.
    [18] Ren CJ, Zhang Y, Cui WZ, et al. A polysaccharide extract of mulberry leaf ameliorates hepatic glucose metabolism and insulin signaling in rats with type 2 diabetes induced by high fat-diet and streptozotocin[J]. Int J Biol Macromol, 2015, 72: 951-959.
    [19] Tu J, Liu GH, Cao XT, et al. Hypoglycemic effects of wheat bran alkyresorcinols in high-fat/high-sucrose diet and low-dose streptozotocin-induced type 2 diabetic male mice and protection of pancreatic β cells[J]. Food Funct, 2019, 10(6): 3282-3290.
    [20] Rydén L, Gyberg V, Schnell O, et al. Oral glucose tolerance testing and cardiovascular disease[J]. Lancet Diabetes Endocrinol, 2016, 4(9): 732-733.
    [21] Liu L, Tang D, Zhao HQ, et al. Hypoglycemic effect of the polyphenols rich extract from Rose rugosa Thunb on high fat diet and STZ induced diabetic rats[J]. J Ethnopharmacol, 2017, 200: 174-181.
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出版历程
  • 收稿日期:  2020-07-08
  • 修回日期:  2020-12-24
  • 刊出日期:  2021-02-24

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