microRNA-23b对Aβ<sub>25-35</sub>导致神经细胞凋亡的作用及机制研究

潘可萌; 陈松; 高向东; 姚文兵

doi:10.11665/j.issn.1000-5048.20210411

microRNA-23b对Aβ_25-35导致神经细胞凋亡的作用及机制研究

中国药科大学生命科学与技术学院江苏省生物药物成药性研究重点实验室，南京 211198

基金项目: 国家自然科学基金资助项目(No.81872850,No.81673435)；中国药科大学“双一流”大学建设资助项目(No.CPU2018GF08)

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出版历程
- 收稿日期: 2020-12-27
- 修回日期: 2021-04-26
- 刊出日期: 2021-08-24

Effects and mechanisms of microRNA-23b on neuronal apoptosis induced by Aβ_25-35

Jiangsu Key Laboratory of Druggability of Biopharmaceuticals, School of Life Science and Technology, China Pharmaceutical University, Nanjing 211198, China

Funds: This study was supported by the National Natural Science Foundation of China (No.81872850, No.81673435) and the "Double First-Class" Project of China Pharmaceutical University (No.CPU2018GF08)

摘要

摘要: 为了研究miR-23b对阿尔茨海默病（Alzheimer′s disease，AD）细胞模型的作用及机制，采用Aβ_25-35诱导SH-SY5Y细胞损伤建立细胞模型；采用Annexin V-FITC/PI双染结合流式细胞术检测转染miR-23b对Aβ_25-35诱导SH-SY5Y细胞凋亡情况的影响；采用JC-1探针检测转染miR-23b对Aβ_25-35损伤SH-SY5Y细胞后细胞内线粒体膜电位的影响；采用Western blot检测转染miR-23b对Aβ_25-35诱导SH-SY5Y细胞内凋亡相关蛋白Bax、Bcl-2以及自噬相关蛋白p62、LC3B、Beclin-1表达水平的变化。结果显示：miR-23b能够改善Aβ_25-35诱导SH-SY5Y细胞的凋亡水平和异常的线粒体膜电位，并缓解Aβ_25-35引起的神经细胞凋亡蛋白和自噬蛋白表达的异常，提示miR-23b能够改善Aβ_25-35导致的凋亡通路和自噬通路异常，进而缓解Aβ_25-35导致的神经细胞凋亡。
- 微小RNA-23b /
- 阿尔茨海默病 /
- β-淀粉样蛋白 /
- 凋亡 /
- 自噬
Abstract: In this study, the effects and mechanisms of miR-23b in the AD cell model were explored. Aβ_25-35 was used to induce neuronal injury model, and cell viabilities were detected by MTT assay. The effect of miR-23b on the apoptotic levels of Aβ_25-35-induced SH-SY5Y cells was analyzed using Annexin V-FITC/PI detection kit. The effect of miR-23b on the mitochondrial membrane potential of Aβ_25-35-induced SH-SY5Y cells was examined using JC-1 fluorescent probe. The levels of cell apoptosis-related proteins and autophagy-related proteins were detected by Western blot. The results showed that miR-23b could alleviate the apoptosis and the abnormal mitochondrial membrane potential in SH-SY5Y cells induced by Aβ_25-35.This study suggested that miR-23b may attenuate Aβ_25-35-induced neuronal apoptosis by regulating apoptosis and autophagy-related pathway.
- microRNA-23b /
- Alzheimer′s disease /
- β-amyloid /
- apoptosis /
- autophagy

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参考文献(32)

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microRNA-23b对Aβ25-35导致神经细胞凋亡的作用及机制研究

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出版历程

Effects and mechanisms of microRNA-23b on neuronal apoptosis induced by Aβ25-35

计量

出版历程

目录

microRNA-23b对Aβ_25-35导致神经细胞凋亡的作用及机制研究

Effects and mechanisms of microRNA-23b on neuronal apoptosis induced by Aβ_25-35