芒柄花素-3′-磺酸钠对胶原性类风湿关节炎的治疗作用及其机制
Therapeutic effect and mechanism of sodium formononetin-3′-sulphonate on collagen-induced rheumatoid arthritis
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摘要: 探讨芒柄花素-3′-磺酸钠对小鼠胶原性类风湿关节炎的治疗作用及其机制。采用鸡Ⅱ型胶原诱导C57小鼠成功建立了类风湿关节炎（collagen-induced arthritis，CIA）模型，腹腔注射不同剂量的芒柄花素-3′-磺酸钠（50，100，200 mg/kg），治疗前后观察所有组小鼠体重、食物摄入量和足肿胀情况。CBA试剂盒检测血清中TNF-α、IL-6、IL-10的水平，蛋白质印迹法测定脾脏组织中NF-κB p65、p-NF-κB p65 （p-p65）、TIPE2、PCNP、IκB-α的表达水平，同时观察小鼠的脏器指数、踝关节软骨组织的病理变化及踝关节组织中NF-κB p65阳性表达情况。结果表明，与模型对照组比较，给药组小鼠体重及食物摄入量有所升高，足肿胀程度降低；血清中炎症因子TNF-α、IL-6表达水平降低，抑炎因子IL-10表达上升；脾组织中NF-κB p65、p-p65、PCNP蛋白表达降低，而TIPE2、IκB-α蛋白表达上升；给药组CIA小鼠的脾和胸腺指数降低，踝关节中炎细胞浸润减少，滑膜组织及软骨破坏程度减轻，NF-κB p65阳性表达水平下降；其中芒柄花素-3′-磺酸钠高剂量组显示出更好的治疗效果。结果提示芒柄花素-3′-磺酸钠对CIA小鼠具有治疗作用，其作用机制可能是通过调控NF-κB p65信号通路，抑制炎症因子水平表达来实现的。
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关键词:
- 芒柄花素-3′-磺酸钠 /
- 胶原性类风湿关节炎 /
- NF-κB p65 /
- 作用机制
Abstract: To investigate the therapeutic effect and mechanism of sodium formononetin-3′-sulphonate (SFS) on collagen-induced rheumatoid arthritis in mice, C57 mice were induced with chicken type II collagen to establish a model of rheumatoid arthritis (collagen-induced arthritis, CIA), and were injected intraperitoneally with different doses of SFS (50,100,200 mg/kg). Body weight, food intake and foot swelling of all groups were observed during the experiment.After the treatment, TNF-α, IL-6, and IL-10 in the serum were detected with the CBA kit; NF-κB p65, p-NF-κB p65 (p-p65), TIPE2, PCNP and IκB-α in spleen tissue were determined by Western blot; the organ index, pathological changes of ankle joint cartilage tissue and the positive expression of NF-κB p65 in ankle joint tissue were also observed.The results showed that, compared with the model group, the body weight and food intake of mice in the treatment group increased, while the degree of foot swelling decreased; the expression levels of inflammatory factors TNF-α and IL-6 in serum decreased, while the expression of anti-inflammatory factor IL-10 increased; the expression levels of NF-κB p65, p-p65 and PCNP in spleen tissue decreased, while the expression of TIPE2 and IκB-α protein increased; the index of spleen and thymus of the CIA mice in the treatment group, the infiltration of inflammatory cells in the ankle joint, the destruction of synovial tissue and cartilage, and the positive expression of NF-κB p65 decreased.Among them, the high-dose group of SFS showed a better therapeutic effect.It is suggested that SFS has a therapeutic effect on CIA mice, and the mechanism may be achieved by regulating the NF-κB p65 signaling pathway and inhibiting the expression of inflammatory factors. -
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