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灵芝孢子糖肽对小鼠肝损伤的保护作用

Protective effect of Ganoderma lucidum spore glycopeptide on liver injury in mice

  • 摘要: 研究灵芝孢子糖肽对小鼠肝损伤的保护作用。分别建立四氯化碳(CCl4)、乙醇致小鼠急性、慢性肝损伤,灌胃给予灵芝孢子糖肽65,130,260 mg/kg,测定小鼠肝指数,血清谷草转氨酶(AST)、谷丙转氨酶(ALT),ELISA法检测肝组织炎症因子白介素-6(IL-6)、肿瘤坏死因子(TNF-α)、诱导型一氧化氮合酶(iNOS)含量,HE染色观察肝组织病理损伤。结果表明,灵芝孢子糖肽可显著降低急性、慢性肝损伤小鼠肝指数、血清AST和ALT含量。在CCl4致慢性肝损伤模型中,灵芝孢子糖肽显著降低小鼠肝组织IL-6、TNF-α及iNOS的含量,改善肝组织病理损伤。上述结果表明,灵芝孢子糖肽可以显著减轻小鼠急、慢性肝损伤,并且具有一定的抗炎活性。

     

    Abstract: Models of acute and chronic liver injury in mice were established using carbon tetrachloride (CCl4) and ethanol to explore the protective effects of Ganoderma lucidum spore glycopeptide on liver injury.Different dosage of Ganoderma lucidum spore glycopeptide (65,130,260 mg/kg) were given by gavage.The liver index and the levels of serum aspartate transaminase (AST) and alanine transaminase (ALT) were determined.The contents of liver interleukin-6 (IL-6), tumor necrosis factor (TNF-α) and inducible nitric oxide synthase (iNOS) were tested by enzyme-linked immunosorbent assay (ELISA).The pathological injury of liver tissue was observed by HE staining.The results showed that Ganoderma lucidum spore glycopeptide could significantly reduce the liver index and the contents of serum AST and ALT in mice of acute and chronic liver injury.In mice of chronic liver injury induced by CCl4, Ganoderma lucidum spore glycopeptide could significantly decrease the contents of liver IL-6, TNF-α and iNOS, and alleviate the pathological damage of liver tissue.Results suggested that Ganoderma lucidum spore glycopeptide might reduce acute and chronic liver injury with anti-inflammatory effects in mice.

     

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