Abstract: Due to oxidative stress, infection, vascular disease, inflammation and neuropathy caused by metabolic disorders induced by hyperglycemia, Diabetes mellitus (DM) patients often suffer from poor wound healing and develop diabetic foot ulcers (DFUs), which may even lead to amputation in severe cases.Through the deep understanding of the diabetic wound healing process and its pathophysiology, effective strategies for the treatment of wound injury can be obtained.Nano-preparation has been widely used in accelerating wound healing in recent years.On the one hand, nanoparticles are beneficial for diabetic wound healing; on the other hand, they can be used to deliver one or more therapeutic agents such as growth factors, nucleic acids, antibiotics and antioxidants, which can be continuously released within the target tissue.This paper reviews the application and mechanism of various nano-preparation in promoting diabetic wound healing.