毛发中地佐辛、杜冷丁的UPLC-MS/MS检测

施晓露; 乔宏伟; 吴健美; 狄斌; 王优美

doi:10.11665/j.issn.1000-5048.20220111

毛发中地佐辛、杜冷丁的UPLC-MS/MS检测

施晓露^1,2,
乔宏伟^2,3,
吴健美^2,3,
狄斌^1,2,
王优美^2,3

1.
中国药科大学药学院，南京 210009
2.
毒品监测管控与禁毒关键技术公安部重点实验室，北京 100741
3.
公安部禁毒情报技术中心，北京 100741

基金项目: 公安部科技强警基础工作专项资助项目（No.2020GABJC22）

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出版历程
- 收稿日期: 2021-04-09
- 修回日期: 2021-11-28
- 刊出日期: 2022-02-24

Determination of dezocine and pethidine in human hair by UPLC-MS/MS

1.
School of Pharmacy, China Pharmaceutical University, Nanjing 210009
2.
Key Laboratory of Drug Monitoring and Control, Beijing 100741
3.
Drug Intelligence and Forensic Center, Ministry of Public Security of the People Republic of China, Beijing 100741, China

Funds: This study was supported by the Special Project of Basic Work of Strengthening Police by Science and Technology of Ministry of Public Security (No. 2020GABJC22)

摘要

摘要: 建立快速检测毛发中地佐辛和杜冷丁含量的超高效液相色谱-质谱（UPLC-MS/MS）法。将清洗后的毛发样品经甲醇研磨超声提取后进行UPLC-MS/MS分析。色谱柱为Waters Acquity BEH C₁₈（2.1 mm × 100 mm，1.7 μm），流动相为0.1%甲酸水-甲醇，0.4 mL/min梯度洗脱。质谱采用ESI⁺离子源，MRM多反应监测，测定地佐辛和杜冷丁的定性离子对和定量离子对。地佐辛和杜冷丁在0.01 ~ 8 ng/mg范围内线性关系良好，检出限均为0.005 ng/mg，定量限均为0.01 ng/mg；准确度和精密度、基质效应和提取回收率以及稳定性均符合方法学要求。本研究建立的方法可简单、快速、准确地测定毛发中地佐辛和杜冷丁的含量，可应用于地佐辛和杜冷丁滥用的相关案件分析。
- 地佐辛 /
- 杜冷丁 /
- 毛发检测 /
- 超高效液相色谱-质谱联用技术
Abstract: A rapid analytical method for the determination of dezocine and pethidine in hair samples using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was established.After cleaned hair was extracted by grinding with methanol and ultrasonic, the final solution was analyzed by UPLC-MS/MS.The targets were gradient eluted on a Waters Acquity BEH C₁₈ (2.1 mm × 100 mm, 1.7 μm) column with 0.1% formic acid-water and methanol as mobile phase at a flow rate of 0.4 mL/min.The ESI⁺ion source and multiple reaction monitoring (MRM) were used to select the qualitative and quantitative ion pairs of dezocine and pethidine.Dezocine and pethidine showed good linearity in the range of 0.01-8 ng/mg, with the limit of detection of 0.005 ng/mg and the LOQs of 0.01 ng/mg.The accuracy, precision, matrix effect, extraction recovery, and stability all met the requirements.The established method is simple, rapid, and accurate for the qualitative and quantitative determination of dezocine and pethidine in hair, which can be applied in the case analysis of dezocine and/or pethidine abuse.
- dezocine /
- pethidine /
- hair analysis /
- UPLC-MS/MS

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参考文献(15)

[1]	. Shanghai Med Pharm J（上海医药），2020，41（7）：24-27.
[2]	Zhou XL，Zhang CJ，Wang M，et al. Dezocine for preventing postoperative pain：a meta-analysis of randomized controlled trials［J］. PLoS One，2015，10（8）：e0136091.
[3]	Zhang XB. Addictive risk of dizosin in pain treatment its control method［J］. Chin J Drug Depend （中国药物依赖性杂志），2020，29（1）：73-77.
[4]	Wu XC，Zhang YC，Zeng JL，et al. Clinical analysis of 8 drug dependence with dezocine［J］. Chin J Drug Depend （中国药物依赖性杂志），2020，29（6）：474-476.
[5]	Xu J，Sun P，Li J，et al. Risk of pethidine addiction and preventive measures［J］. Chin J Drug Depend （中国药物依赖性杂志），2020，29（6）：477-480.
[6]	Kul A，Ozdemir M，Sagirli O. Determination of pethidine of abuse and relevant metabolite norpethidine in urine by ultra-performance liquid chromatography-tandem mass spectrometry［J］. J Pharm Biomed Anal，2020，186：113320.
[7]	Cui ZM，Wang YY，Jiao FF，et al. A sensitive HPLC MS/MS method for determination of dezocine in human plasma and its application［J］. Pharm Clin Res（药学与临床研究），2018，26（1）：14-17.
[8]	Zhang KJ. Determination of dolantin in whole blood by SPE/GC-MS［J］. Guangzhou Chem Ind（广州化工），2016，44（23）：100-101，134.
[9]	Li SY，Chen XY. Simultaneous determination of dexmedetomidine and dezocine in human plasma by HPLC-MS/MS［J］. Chin J Clin Pharmacol（中国临床药理学杂志），2020，36（19）：3118-3121.
[10]	Kuwayama K，Miyaguchi H，Iwata YT，et al. Strong evidence of drug-facilitated crimes by hair analysis using LC-MS/MS after micro-segmentation［J］. Forensic Toxicol，2019，37（2）：480-487.
[11]	Kintz P. Hair analysis in forensic toxicology［J］. Wires Forensic Sci，2019，1（1）. doi：10.1002/wfs2.1196.
[12]	Wang T，Shen BH，Wu HJ，et al. Disappearance of R/S-methamphetamine and R/S-amphetamine from human scalp hair after discontinuation of methamphetamine abuse［J］. Forensic Sci Int，2018，284：153-160.
[13]	Hou W，Zhang LP，Wang JF，et al. Determination and statistics of common drugs and their metabolites in human hair by ultra-performance liquid chromatography coupled with mass spectrometry［J］. Chin J Anal Lab （分析试验室），2020，39（11）：1264-1270.
[14]	Pan SY，Zou QG，Han M，et al. Determination of imidafenacin in human plasma by UPLC-MS/MS and its bioequivalence［J］. J China Pharm Univ（中国药科大学学报），2019，50（5）：579-584.
[15]	Academy of Forensic Science. Detection of 15 illicit drugs and metabolites in hair by liquid chromatography-tandem mass spectrometry（毛发中15种毒品及代谢物的液相色谱-串联质谱检验方法）： SF/Z JD0107025-2018［S］. Beijing：Ministry of Justice of the People''s Republic of China，2018.