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羧甲司坦L-精氨酸盐的合成及其对支气管上皮细胞的保护作用

贾健, 吴建兵, 张奕华, 黄张建

贾健, 吴建兵, 张奕华, 黄张建. 羧甲司坦L-精氨酸盐的合成及其对支气管上皮细胞的保护作用[J]. 中国药科大学学报, 2022, 53(2): 171-177. DOI: 10.11665/j.issn.1000-5048.20220206
引用本文: 贾健, 吴建兵, 张奕华, 黄张建. 羧甲司坦L-精氨酸盐的合成及其对支气管上皮细胞的保护作用[J]. 中国药科大学学报, 2022, 53(2): 171-177. DOI: 10.11665/j.issn.1000-5048.20220206
JIA Jian, WU Jianbing, ZHANG Yihua, HUANG Zhangjian. Synthesis and protective effect of carboxymethylcysteine L-arginate in bronchial epithelial cells[J]. Journal of China Pharmaceutical University, 2022, 53(2): 171-177. DOI: 10.11665/j.issn.1000-5048.20220206
Citation: JIA Jian, WU Jianbing, ZHANG Yihua, HUANG Zhangjian. Synthesis and protective effect of carboxymethylcysteine L-arginate in bronchial epithelial cells[J]. Journal of China Pharmaceutical University, 2022, 53(2): 171-177. DOI: 10.11665/j.issn.1000-5048.20220206

羧甲司坦L-精氨酸盐的合成及其对支气管上皮细胞的保护作用

基金项目: 国家自然科学基金资助项目(No.81822041,No.82173681,No.21977116,No.82104004,No.81773573)

Synthesis and protective effect of carboxymethylcysteine L-arginate in bronchial epithelial cells

Funds: This study was supported by the National Natural Science Foundation of China (No.81822041, No.82173681, No.21977116,No.82104004, No.81773573)
  • 摘要: 羧甲司坦(CMC)是治疗慢性阻塞性肺病的常用药,长期服用对胃肠道产生严重刺激。L-精氨酸是一氧化氮(NO)合酶(NOS)的底物,在体内可转化为对心血管及胃肠道等有益的NO。L-精氨酸属碱性氨基酸,能与某些含羧酸基团的化合物成盐以改善原药的水溶性,并可能由于促进NO的释放带来活性的提高或毒副作用的缓解。因此,本文设计、合成了CMC的L-精氨酸盐(CMCA),并测试其理化性质以及在香烟烟雾诱导的人支气管上皮细胞损伤模型中清除活性氧(ROS)、抗细胞凋亡和NO释放的能力。结果表明,CMCA能有效捕获ROS,释放NO,并抑制细胞凋亡,效果优于CMC或L-精氨酸,提示该化合物值得深入研究和开发。
    Abstract: Carboxymethylcysteine (CMC) is a common drug for the clinical treatment of chronic obstructive pulmonary disease, yet its long-term use can cause severe irritation to the gastrointestinal tract.As the substrate of nitric oxide (NO) synthase (NOS), L-arginine can be converted in the body into NO beneficial to the cardiovascular system, the gastrointestinal tract and so on.As a basic amino acid, L-arginine can be salified with some compounds containing acidic groups to improve the water solubility of the parent drug and may enhance the activity and alleviate side effects due to NO release.In this study, we designed and synthesized carboxymethylcysteine L-arginate (CMCA), and tested its physico-chemical properties, and the abilities to scavenge reactive oxygen species (ROS), inhibit apoptosis and release NO in cigarette smoke-induced injury model of human bronchial epithelial cells.The results revealed that CMCA is superior to CMC or L-arginine in that it could capture ROS, release NO and suppress apoptosis, suggesting that CMCA is worthy of further research and development.
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出版历程
  • 收稿日期:  2021-12-18
  • 修回日期:  2022-03-14
  • 刊出日期:  2022-04-24

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