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HPLC-DPPH在线联用技术评价制首乌的抗氧化活性

钱金珠, 梁明, 余意, 邓亚雷, 刘史佳, 庞会明, 戚进

钱金珠, 梁明, 余意, 邓亚雷, 刘史佳, 庞会明, 戚进. HPLC-DPPH在线联用技术评价制首乌的抗氧化活性[J]. 中国药科大学学报, 2022, 53(2): 185-191. DOI: 10.11665/j.issn.1000-5048.20220208
引用本文: 钱金珠, 梁明, 余意, 邓亚雷, 刘史佳, 庞会明, 戚进. HPLC-DPPH在线联用技术评价制首乌的抗氧化活性[J]. 中国药科大学学报, 2022, 53(2): 185-191. DOI: 10.11665/j.issn.1000-5048.20220208
QIAN Jinzhu, LIANG Ming, YU Yi, DENG Yalei, LIU Shijia, PANG Huiming, QI Jin. Determination of antioxidant activity of Polygoni Multiflori Radix Praeparata by HPLC-DPPH[J]. Journal of China Pharmaceutical University, 2022, 53(2): 185-191. DOI: 10.11665/j.issn.1000-5048.20220208
Citation: QIAN Jinzhu, LIANG Ming, YU Yi, DENG Yalei, LIU Shijia, PANG Huiming, QI Jin. Determination of antioxidant activity of Polygoni Multiflori Radix Praeparata by HPLC-DPPH[J]. Journal of China Pharmaceutical University, 2022, 53(2): 185-191. DOI: 10.11665/j.issn.1000-5048.20220208

HPLC-DPPH在线联用技术评价制首乌的抗氧化活性

基金项目: 江苏省中医药科技发展专项资助项目(No.2020ZX11);江苏省中医药管理局科技资助项目(No.JD2019SZ14)

Determination of antioxidant activity of Polygoni Multiflori Radix Praeparata by HPLC-DPPH

Funds: This study was supported by Chinese Medicine Technology Development Project of Jiangsu Province (No.2020ZX11) and the Science and Technology Project of Jiangsu Administration of Traditional Chinese Medicine (No.JD2019SZ14)
  • 摘要: 建立高效液相色谱-1,1-二苯基-2-三硝基苯肼(HPLC-DPPH)在线联用技术对3种不同炮制方法的制首乌抗氧化活性进行了评价。通过分析16批制首乌样品的化学指纹峰(峰)和活性指纹峰(效),建立量效关系方程,并以活性加和的方式量化各批次何首乌的抗氧化总活性。结果表明,所建立的HPLC-DPPH在线联用技术可评价不同批次不同炮制方法的制首乌抗氧化活性,为制首乌的质量评价提供了数据支持和科学依据。
    Abstract: An on-line HPLC-DPPH system was developed to determine the antioxidant activity of 16 batches of Polygoni Multiflori Radix Praeparata. By analyzing the chromatographic and biological activity fingerprints of 16 batches of Polygoni Multiflori Radix Praeparata, the dose-effect relationship was established and the total antioxidant activity was quantified by activity addition.The results suggested that the online HPLC-DPPH method can evaluate the antioxidant activity of different bathches of Polygoni Multiflori Radix Praeparata, with different processing methods, aiming to provide datasupport and scientific basis forquality evaluation of Polygoni Multiflori Radix Praeparata.
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  • 期刊类型引用(1)

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出版历程
  • 收稿日期:  2021-10-10
  • 修回日期:  2022-03-03
  • 刊出日期:  2022-04-24

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