HPLC-DPPH在线联用技术评价制首乌的抗氧化活性

Determination of antioxidant activity of Polygoni Multiflori Radix Praeparata by HPLC-DPPH

摘要: 建立高效液相色谱-1，1-二苯基-2-三硝基苯肼（HPLC-DPPH）在线联用技术对3种不同炮制方法的制首乌抗氧化活性进行了评价。通过分析16批制首乌样品的化学指纹峰（峰）和活性指纹峰（效），建立量效关系方程，并以活性加和的方式量化各批次何首乌的抗氧化总活性。结果表明，所建立的HPLC-DPPH在线联用技术可评价不同批次不同炮制方法的制首乌抗氧化活性，为制首乌的质量评价提供了数据支持和科学依据。

Abstract: An on-line HPLC-DPPH system was developed to determine the antioxidant activity of 16 batches of Polygoni Multiflori Radix Praeparata. By analyzing the chromatographic and biological activity fingerprints of 16 batches of Polygoni Multiflori Radix Praeparata, the dose-effect relationship was established and the total antioxidant activity was quantified by activity addition.The results suggested that the online HPLC-DPPH method can evaluate the antioxidant activity of different bathches of Polygoni Multiflori Radix Praeparata, with different processing methods, aiming to provide datasupport and scientific basis forquality evaluation of Polygoni Multiflori Radix Praeparata.