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斑马鱼模型在新精神活性物质代谢研究中的应用

许琳灏, 刘信泽, 刘伟, 向平, 杭太俊, 阳硕, 严慧

许琳灏, 刘信泽, 刘伟, 向平, 杭太俊, 阳硕, 严慧. 斑马鱼模型在新精神活性物质代谢研究中的应用[J]. 中国药科大学学报, 2022, 53(5): 606-612. DOI: 10.11665/j.issn.1000-5048.20220511
引用本文: 许琳灏, 刘信泽, 刘伟, 向平, 杭太俊, 阳硕, 严慧. 斑马鱼模型在新精神活性物质代谢研究中的应用[J]. 中国药科大学学报, 2022, 53(5): 606-612. DOI: 10.11665/j.issn.1000-5048.20220511
shu
XU Linhao, LIU Xinze, LIU Wei, XIANG Ping, HANG Taijun, YANG Shuo, YAN Hui. Application of zebrafish model in the metabolism of new psychoactive substances[J]. Journal of China Pharmaceutical University, 2022, 53(5): 606-612. DOI: 10.11665/j.issn.1000-5048.20220511
Citation: XU Linhao, LIU Xinze, LIU Wei, XIANG Ping, HANG Taijun, YANG Shuo, YAN Hui. Application of zebrafish model in the metabolism of new psychoactive substances[J]. Journal of China Pharmaceutical University, 2022, 53(5): 606-612. DOI: 10.11665/j.issn.1000-5048.20220511
shu

斑马鱼模型在新精神活性物质代谢研究中的应用

  • 1.

    司法鉴定科学研究院 上海市法医学重点实验室 上海市司法鉴定专业技术服务平台 司法部司法鉴定重点实验室，上海 200063

  • 2.

    中国药科大学药学院，南京 210009

基金项目: 上海市人才发展资助项目（No.2019095）；上海市自然科学基金资助项目（No.19ZR1458800）；上海市法医学重点实验室资助项目（No.21DZ2270800）；上海市司法鉴定专业技术服务平台资助项目（No.19DZ2292700）

Application of zebrafish model in the metabolism of new psychoactive substances

  • 1.

    Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Science Platform, Key Laboratory of Forensic Science, Ministry of Justice, Academy of Forensic Science, Shanghai 200063

  • 2.

    School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China

Funds: This study was supported by Shanghai Talent Development Project (No.2019095); the Natural Science Foundation of Shanghai (No.19ZR1458800); the Project of Shanghai Key Laboratory of Forensic Medicine (No.21DZ2270800); and the Project of Shanghai Judicial Expertise Professional and Technical Service Platform (No.19DZ2292700)

摘要

    摘要
  • 摘要: 斑马鱼具有体型小、易于饲养、繁殖成本低、繁殖率高等诸多优势，是药物代谢研究的新型模式生物。本文概述斑马鱼代谢模型的构建，介绍斑马鱼模型的基因与人类高度相似性以及其发达的酶系统、较低的基质效应等优点；总结斑马鱼模型在合成大麻素类、芬太尼类、哌嗪类、合成卡西酮类等各类新精神活性物质研究中的应用；综述斑马鱼中体内常见的代谢反应包括Ⅰ相（氧化、N-脱甲基、O-脱乙基化、羟化和N-脱烷基）和Ⅱ相（硫酸化和葡萄糖醛酸化）反应，说明其代谢物种类与真实人体体液样本之间的差异主要和酶的种类有关，指出斑马鱼模型在研究新精神活性物质方面的巨大潜力和进一步研究趋势。
    Abstract: Zebrafish is a novel model organism for drug metabolism research because of its small size, easy feeding, low reproduction cost and high reproduction rate.This paper outlines the construction of zebrafish metabolic model, illustrating the advantages of zebrafish model with high genetic similarity to human, well-developed enzyme system, and low substrate effect; and then reviews the application of zebrafish model in the synthesis of various new psychoactive substances such as cannabinoids, fentanyl, piperazines, and synthetic cathinones.The common metabolic reactions in zebrafish, including phase I (oxidation, N-demethylation, O-deethylation, hydroxylation and N-desalkylation) and phase II (sulfation and glucuronidation) reactions, are summarized, and the differences between the metabolites of zebrafish and those of real human body fluid samples are mainly related to the types of enzymes.This paper also points out the great potential and further research trends of zebrafish model in the study of new psychoactive substances.
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  • 收稿日期:  2022-05-09
  • 修回日期:  2022-09-12
  • 刊出日期:  2022-10-24

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