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新型嗪类荧光染料用于术中神经成像

包瑞楚, 李昌盛, 顾月清

包瑞楚, 李昌盛, 顾月清. 新型嗪类荧光染料用于术中神经成像[J]. 中国药科大学学报, 2022, 53(6): 716-724. DOI: 10.11665/j.issn.1000-5048.20220611
引用本文: 包瑞楚, 李昌盛, 顾月清. 新型嗪类荧光染料用于术中神经成像[J]. 中国药科大学学报, 2022, 53(6): 716-724. DOI: 10.11665/j.issn.1000-5048.20220611
BAO Ruichu, LI Changsheng, GU Yueqing. Novel oxazine fluorescent dyes for intraoperative neuroimaging[J]. Journal of China Pharmaceutical University, 2022, 53(6): 716-724. DOI: 10.11665/j.issn.1000-5048.20220611
Citation: BAO Ruichu, LI Changsheng, GU Yueqing. Novel oxazine fluorescent dyes for intraoperative neuroimaging[J]. Journal of China Pharmaceutical University, 2022, 53(6): 716-724. DOI: 10.11665/j.issn.1000-5048.20220611

新型嗪类荧光染料用于术中神经成像

Novel oxazine fluorescent dyes for intraoperative neuroimaging

  • 摘要: 利用神经特异性荧光剂作为动物的神经标志物用于指导手术操作,可降低术中神经损伤的发生率。本研究在嗪母核结构的基础上进行结构修饰,通过质谱和核磁共振氢谱证实得到一系列嗪衍生物荧光染料YQN-3~YQN-6,以突出大鼠的周围神经结构。在一系列靶向荧光染料中,YQN-3在NIR附近具有发射峰值,静脉注射4 h后在臂丛神经和坐骨神经中显示出高特异性神经靶向信号。此外,在甲状腺切除术中,YQN-3能精准定位并识别出喉返神经,从而在术中保留这些神经的完整性。YQN-3合成工艺简单,毒性小,具有潜在的临床神经组织显像应用前景。
    Abstract: Nerve-specific fluorescent agents can be used as nerve markers in animals to guide surgical procedures and reduce the incidence of intraoperative nerve injury.In this study, the structure of oxazine mother nucleus was modified.A series of oxazine derivative fluorescent dyes YQN-3-YQN-6 were obtained by mass spectrometry and 1H NMR, which can highlight the peripheral nerve structure of rats.Among a series of targeted fluorescent dyes, YQN-3 had emission peaks near NIR and showed highly specific nerve targeting signals in the brachial plexus and sciatic nerves 4 h after intravenous administration.In addition, YQN-3 can accurately locate and identify recurrent laryngeal nerves during thyroidectomy, thus preserving the integrity of these nerves during surgery.With its simple synthesis and low toxicity, YQW-3 can be potentially applied for clinical neural tissue imaging.
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出版历程
  • 收稿日期:  2022-06-16
  • 修回日期:  2022-11-16
  • 刊出日期:  2022-12-24

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