SNP-9对鱼藤酮所致帕金森病细胞模型的作用及机制研究

黄志欢; 梁潇; 高向东; 陈松

doi:10.11665/j.issn.1000-5048.2023020101

SNP-9对鱼藤酮所致帕金森病细胞模型的作用及机制研究

中国药科大学生命科学与技术学院江苏省生物药物成药性研究重点实验室，南京 211198

基金项目: 国家自然科学基金资助项目（No.82073755；No.82173728）；国家级大学生创新创业训练计划项目

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- 收稿日期: 2023-01-31
- 修回日期: 2023-03-20
- 刊出日期: 2023-04-24

Effects and mechanisms of SNP-9 on Parkinson''s disease cell model induced by rotenone

Jiangsu Provincial Key Laboratory of Druggability of Biopharmaceuticals, School of Life Science and Technology, China Pharmaceutical University, Nanjing 211198, China

Funds: This study was supported by the National Natural Science Foundation of China (No. 82073755, No. 82173728); and the National Innovation and Entrepreneurship Training Program for Undergraduate

摘要

摘要: 本文研究SNP-9对帕金森病（Parkinson’s disease， PD）细胞模型的作用和机制。使用鱼藤酮损伤SH-SY5Y细胞建立PD细胞模型；通过MTT法检测鱼藤酮和SNP-9对细胞活力的影响；Hoechst/PI双染法检测鱼藤酮和SNP-9对细胞凋亡的影响；DCFH-DA探针检测鱼藤酮和SNP-9对细胞活性氧（reactive oxygen species， ROS）水平的影响；Western blot检测鱼藤酮和SNP-9对酪氨酸羟化酶（tyrosine hydroxylase， TH）、α-突触核蛋白（α-synuclein， α-syn）、Bcl-2、Bax蛋白水平的影响。研究结果显示，SNP-9能够缓解鱼藤酮诱导的SH-SY5Y细胞活力、TH和α-syn水平、细胞凋亡、ROS水平，以及细胞凋亡相关蛋白Bax/Bcl-2的异常。研究结果提示SNP-9可能通过调控凋亡相关通路，进而缓解鱼藤酮导致的神经细胞损伤。
- 帕金森病 /
- SNP-9 /
- 鱼藤酮 /
- 凋亡
Abstract: In this article, the effects and mechanisms of SNP-9 on Parkinson''s disease (PD) cell model were investigated.SH-SY5Y cells were treated with rotenone to establish PD cell model; the effects of rotenone and SNP-9 on cell viability were detected by MTT assay; Hoechst/PI double staining assay was used to detect the effects of rotenone and SNP-9 on cell apoptosis; DCFH-DA probe was used to detect the effects of rotenone and SNP-9 on cellular reactive oxygen species (ROS) levels; and Western blot was used to detect the effects of rotenone and SNP-9 on protein levels of tyrosine hydroxylase (TH), α-synuclein (α-syn), Bcl-2 and Bax.The results showed that SNP-9 could alleviate abnormalities in cell viability, levels of TH and α-syn, apoptosis, ROS and apoptotic relative protein Bax/Bcl-2 induced by rotenone.Our findings suggest that SNP-9 may alleviate rotenone-induced injury in neuronal cells by regulating cell apoptosis related pathway.
- Parkinson''s disease /
- SNP-9 /
- rotenone /
- apoptosis

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参考文献(21)

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SNP-9对鱼藤酮所致帕金森病细胞模型的作用及机制研究

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Effects and mechanisms of SNP-9 on Parkinson''s disease cell model induced by rotenone

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