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M2巨噬细胞来源的外泌体转运miR-1260b促进胶质母细胞瘤迁移的机制研究

张衡, 陈倩倩, 桂艳萍, 黄敏, 郭雅冰, 赵丽

张衡, 陈倩倩, 桂艳萍, 黄敏, 郭雅冰, 赵丽. M2巨噬细胞来源的外泌体转运miR-1260b促进胶质母细胞瘤迁移的机制研究[J]. 中国药科大学学报, 2023, 54(1): 95-106. DOI: 10.11665/j.issn.1000-5048.2023020901
引用本文: 张衡, 陈倩倩, 桂艳萍, 黄敏, 郭雅冰, 赵丽. M2巨噬细胞来源的外泌体转运miR-1260b促进胶质母细胞瘤迁移的机制研究[J]. 中国药科大学学报, 2023, 54(1): 95-106. DOI: 10.11665/j.issn.1000-5048.2023020901
ZHANG Heng, CHEN Qianqian, GUI Yanping, HUANG Min, GUO Yabing, ZHAO Li. Mechanism of M2 macrophage-derived exosomes in promoting the migration of glioblastoma via transferring miR-1260b[J]. Journal of China Pharmaceutical University, 2023, 54(1): 95-106. DOI: 10.11665/j.issn.1000-5048.2023020901
Citation: ZHANG Heng, CHEN Qianqian, GUI Yanping, HUANG Min, GUO Yabing, ZHAO Li. Mechanism of M2 macrophage-derived exosomes in promoting the migration of glioblastoma via transferring miR-1260b[J]. Journal of China Pharmaceutical University, 2023, 54(1): 95-106. DOI: 10.11665/j.issn.1000-5048.2023020901

M2巨噬细胞来源的外泌体转运miR-1260b促进胶质母细胞瘤迁移的机制研究

Mechanism of M2 macrophage-derived exosomes in promoting the migration of glioblastoma via transferring miR-1260b

  • 摘要: 肿瘤相关巨噬细胞浸润肿瘤组织并促进胶质母细胞瘤进展,但作用机制还有待进一步研究。本文以探究M2巨噬细胞通过分泌外泌体影响胶质母细胞瘤迁移能力的机制为目的。采用超高速离心法提取外泌体;采用RNA测序筛选差异表达的miRNA;采用数据库靶点预测miRNA可能的靶蛋白;采用双萤光素酶报告基因实验验证miRNA与靶基因的相互作用;采用裸鼠皮下移植瘤模型检测肿瘤细胞增殖能力。实验结果表明,肿瘤相关巨噬细胞主要是M2巨噬细胞,M2巨噬细胞分泌的外泌体能够促进脑胶质瘤细胞的迁移,其机制与转运miR-1260b且靶向调控AJAP1影响脑胶质瘤细胞的迁移有关,提示巨噬细胞分泌的外泌体通过转运miR-1260b,从而影响胶质母细胞瘤的迁移能力。
    Abstract: Tumor-associated macrophage promotes the progression of glioblastoma (GBM) by infiltrating into tumor tissue, yet its mechanism has not been fully elucidated.This paper aimed to investigate the mechanism of M2 macrophages in affecting the migratory capacity of GBM via secreting exosomes.Ultracentrifugation was used to extract exosomes; RNA sequencing was carried out to screen differentially expressed miRNAs; target prediction database was used to predict the possible target proteins of miRNA; Dual-luciferase reporter assay was performed to verify the interaction between miRNA and target genes; and the proliferation ability of tumor cells was detected by subcutaneous xenograft model in nude mice.Results showed that tumor-related macrophages were mainly M2 macrophages, and that exosomes secreted by M2 macrophages could promote the migration of glioma cells.Meanwhile, exosomes secreted by M2 macrophages transported miR-1260b and affected the migration of glioma cells through directly targeted AJAP1, suggesting that exosomes secreted by macrophages could affect the migration ability of GBM through transporting miR-1260b.
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出版历程
  • 收稿日期:  2023-02-08
  • 修回日期:  2023-02-22
  • 刊出日期:  2023-02-24

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