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基于荧光素酶生物发光检测方法的研究及其应用进展

刘琳, 胡婷婷, 王梦灵, 聂耀, 张惟杰, 王琛, 邹秉杰, 宋沁馨, 周国华

刘琳, 胡婷婷, 王梦灵, 聂耀, 张惟杰, 王琛, 邹秉杰, 宋沁馨, 周国华. 基于荧光素酶生物发光检测方法的研究及其应用进展[J]. 中国药科大学学报, 2023, 54(4): 389-398. DOI: 10.11665/j.issn.1000-5048.2023021401
引用本文: 刘琳, 胡婷婷, 王梦灵, 聂耀, 张惟杰, 王琛, 邹秉杰, 宋沁馨, 周国华. 基于荧光素酶生物发光检测方法的研究及其应用进展[J]. 中国药科大学学报, 2023, 54(4): 389-398. DOI: 10.11665/j.issn.1000-5048.2023021401
LIU Lin, HU Tingting, WANG Mengling, NIE Yao, ZHANG Weijie, WANG Chen, ZOU Bingjie, SONG Qinxin, ZHOU Guohua. Advance on detection method and application based on bioluminescence of luciferase[J]. Journal of China Pharmaceutical University, 2023, 54(4): 389-398. DOI: 10.11665/j.issn.1000-5048.2023021401
Citation: LIU Lin, HU Tingting, WANG Mengling, NIE Yao, ZHANG Weijie, WANG Chen, ZOU Bingjie, SONG Qinxin, ZHOU Guohua. Advance on detection method and application based on bioluminescence of luciferase[J]. Journal of China Pharmaceutical University, 2023, 54(4): 389-398. DOI: 10.11665/j.issn.1000-5048.2023021401

基于荧光素酶生物发光检测方法的研究及其应用进展

基金项目: 国家自然科学基金资助项目(No.82173780);药物质量与安全预警教育部重点实验室资助项目(No.DQCP20/21MS04);国家级大学生创新创业训练计划项目成果(No.202210316001Z,No.202210316009Z)

Advance on detection method and application based on bioluminescence of luciferase

Funds: This study was supported by the National Natural Science Foundation of China (No.82173780); the Open Project Program of MOE Key Laboratory of Drug Quality Control and Pharmacovigilance (No.DQCP20/21MS04); National Innovation and Entrepreneurship Training Program for Undergraduate (No.202210316001Z, No.202210316009Z)
  • 摘要: 生物发光现象广泛存在于自然界中,不管是在陆地或是海洋都有发光生物的踪迹。其中基于荧光素酶的生物发光系统被广泛研究,启发着人类在基因、表观遗传等方面进行探索,并且开发出一系列相关的检测方法,用于体内体外各方面研究。本文从生物发光系统、荧光素酶的种类以及荧光素酶生物发光检测方法的开发与应用这几个方面进行总结,简要概括近年来基于荧光素酶生物发光检测的研究进展。
    Abstract: Bioluminescence is a widespread phenomenon in nature, and luminescent organisms can be found both on land and in the ocean. Among them, luciferase based bioluminescence systems have been widely studied, inspiring the exploration of genetic and epigenetic aspects and the development of a series of related assays for in vivo and in vitro studies. This paper summarizes the recent developments of luciferase based bioluminescence assays in terms of bioluminescence systems, types of luciferases, and the development and application of luciferase bioluminescence assays.
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出版历程
  • 收稿日期:  2023-02-13
  • 修回日期:  2023-03-15
  • 刊出日期:  2023-08-24

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