稀有人参皂苷对特发性肺纤维化的影响

姚磊; 屈琳琳; 范代娣

doi:10.11665/j.issn.1000-5048.2023042002

稀有人参皂苷对特发性肺纤维化的影响

姚磊^1,2,
屈琳琳^1,2,
范代娣^1,2

1.
西北大学化工学院，西安 710069
2.
西北大学生物医药研究院，西安 710069

基金项目: 国家重点研发计划资助项目（No.2021YFC2101500）；陕西省重点研发计划资助项目（No.2022ZDLSF05-12）

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- 收稿日期: 2023-04-19
- 修回日期: 2023-09-17
- 刊出日期: 2023-10-24

Effects of rare ginsenoside on idiopathic pulmonary fibrosis

YAO Lei^1,2,
QU Linlin^1,2,
FAN Daidi^1,2

1.
School of Chemical Engineering,Northwest University, Xi'an 710069
2.
Biotech &Biomed Research Institute,Northwest University, Xi'an 710069, China

Funds: This study was supported by the National Key Research and Development Program of China (No.2021YFC2101500), and Key Research and Development Program of Shaanxi Province (No.2022ZDLSF05-12)

摘要

摘要: 探究稀有人参皂苷是否可以缓解特发性肺纤维化（idiopathic pulmonary fibrosis，IPF）。体内实验选用C57BL/6小鼠，小鼠分为对照组、博来霉素（bleomycin，BLM）诱导IPF组、稀有人参皂苷Rk1、Rk3、Rh4、Rg5组，除对照组外其余小鼠均腹腔注射BLM 28 d以构建IPF模型，各治疗组同时分别灌胃给予人参皂苷Rk1、Rk3、Rh4、Rg5，实验结束后收集小鼠的肺脏组织，通过苏木精-伊红染色法（HE）观察小鼠肺部的病理变化；测量小鼠肺部组织羟脯氨酸（hydroxyproline，HYP）含量；检测小鼠肺部组织IPF相关基因的表达。体外实验选用人胚肺成纤维细胞（MRC-5），使用 (TGF-β1)诱导IPF细胞模型，通过细胞毒性实验、HYP含量测定和实时荧光定量PCR(RT-qPCR )分析4种皂苷对IPF相关基因表达的影响。4种稀有人参皂苷均能有效缓解IPF引起的肺泡结构破坏等病理进程，降低HYP含量，下调IPF相关基因的表达，表明稀有人参皂苷能够有效缓解IPF。
- 肺纤维化 /
- 稀有人参皂苷 /
- MRC-5细胞 /
- 转化生长因子β1 /
- 博来霉素
Abstract: To investigate whether rare ginsenosides could alleviate idiopathic pulmonary fibrosis (IPF), C57BL/6 mice were randomly divided into control group, bleomycin (BLM)-induced IPF group, rare ginsenoside Rk1 group, rare ginsenoside Rk3 group, rare ginsenoside Rh4 group and rare ginsenoside Rg5 group.All mice except those in the control group were given bleomycin injection.The IPF model was established by BLM for 28 days.The treatment group was given ginsenoside intragastrically at the same time.After the experiment, the lung tissues of mice were collected and the pathological changes of the mice lungs were observed.The content of hydroxyproline (HYP) in mouse lung tissue was measured.The expression of IPF-related genes in mouse lung tissues was detected.In in vitro experiments, Medical Research Council cell strain-5 (MRC-5) was used to induce IPF cell model using transforming growth factor-β1 (10 ng/mL).The effects of four saponins on the expression of IPF-related genes were analyzed by MTT assay, HYP content determination and RT-qPCR.All four rare ginsenosides could effectively alleviate the pathological process such as alveolar structure destruction caused by IPF, reduce the content of HYP, and down-regulate the expression of IPF-related genes, indicating that rare ginsenosides can effectively alleviate IPF.
- pulmonary fibrosis /
- rare ginsenoside /
- MRC-5 cell /
- transforming growth factor β1 /
- bleomycin

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参考文献(26)

[1]	Xie WN, Ding Q, Sun J, et al. Protective effects of Baibu Tang on bleomycin-induced pulmonary fibrosis in mice[J]. J China Pharm Univ (中国药科大学学报), 2018, 49(4): 483-489.
[2]	Steele MP, Schwartz DA. Molecular mechanisms in progressive idiopathic pulmonary fibrosis[J]. Annu Rev Med, 2013, 64: 265-276.
[3]	Miao Y, Wang YH, Bi Z, et al. Antifibrotic mechanism of avitinib in bleomycin-induced pulmonary fibrosis in mice[J]. BMC Pulm Med, 2023, 23(1): 94.
[4]	Xiang J, Yu P, Li MD, et al. Protective effects of stemona alkaloids on mice with bleomycin-induced pulmonary fibrosis[J]. J China Pharm Univ (中国药科大学学报), 2017, 48(1): 76-81.
[5]	Prasse A, Probst C, Bargagli E, et al. Serum CC-chemokine ligand 18 concentration predicts outcome in idiopathic pulmonary fibrosis[J]. Am J Respir Crit Care Med, 2009, 179(8): 717-723.
[6]	Wang J, Xu LZ, Xiang Z, et al. Microcystin-LR ameliorates pulmonary fibrosis via modulating CD206⁺ M2-like macrophage polarization[J]. Cell Death Dis, 2020, 11(2): 136.
[7]	Zhang SN, Li LQ, Zhang HC, et al. Clinical study on treating idiopathic pulmonary fibrosis with TCM method of invigorating Qi, moisturizing the lung and removing blood stasis and toxic substance[J].J Beijing Univ Tradit Chin Med (北京中医药大学学报), 1999, 22(3): 57-60.
[8]	Kang A, Zhang SJ, Shan JJ, et al. Pharmacokinetics of ginsenoside Rb₁ in lincomycin-induced gut microbiota dysbiosis rats[J]. J China Pharm Univ (中国药科大学学报), 2016, 47(2): 182-187.
[9]	Wu W, Jiao CX, Li H, et al. LC-MS based metabolic and metabonomic studies of Panax ginseng[J]. Phytochem Anal, 2018, 29(4): 331-340.
[10]	Liang Y, Zhao S. Progress in understanding of ginsenoside biosynthesis[J]. Plant Biol, 2008, 10(4): 415-421.
[11]	Zhu MR, Yang CL. Blue fluorescent emitters: design tactics and applications in organic light-emitting diodes[J]. Chem Soc Rev, 2013, 42(12): 4963-4976.
[12]	Wang CZ, Aung HH, Ni M, et al. Red American ginseng: ginsenoside constituents and antiproliferative activities of heat-processed Panax quinquefolius roots[J]. Planta Med, 2007, 73(7): 669-674.
[13]	Jin X, Zhu LY, Shen H, et al. Influence of sulphur-fumigation on the quality of white ginseng: a quantitative evaluation of major ginsenosides by high performance liquid chromatography[J]. Food Chem, 2012, 135(3): 1141-1147.
[14]	Pan WL, Xue BL, Yang CL, et al. Biopharmaceutical characters and bioavailability improving strategies of ginsenosides[J]. Fitoterapia, 2018, 129: 272-282.
[15]	Yu T, Rhee MH, Lee J, et al. Ginsenoside rc from Korean red ginseng (Panax ginseng C.A. Meyer) attenuates inflammatory symptoms of gastritis, hepatitis and arthritis[J]. Am J Chin Med, 2016, 44(3): 595-615.
[16]	Leung KW, Pon YL, Wong RN, et al. Ginsenoside-Rg1 induces vascular endothelial growth factor expression through the glucocorticoid receptor-related phosphatidylinositol 3-kinase/Akt and beta-catenin/T-cell factor-dependent pathway in human endothelial cells[J]. J Biol Chem, 2006, 281(47): 36280-36288.
[17]	Bai X, Fu RZ, Duan ZG, et al. Ginsenoside Rh4 alleviates antibiotic-induced intestinal inflammation by regulating the TLR4-MyD88-MAPK pathway and gut microbiota composition[J]. Food Funct, 2021, 12(7): 2874-2885.
[18]	Guan SB, Yu P, Cao JH, et al. Ginsenoside Rg1 protects against cigarette smoke-induced airway remodeling by suppressing the TGF-β1/Smad3 signaling pathway[J]. Am J Transl Res, 2020, 12(2): 493-506.
[19]	Zeng DF, Wang JL, Kong PY, et al. Ginsenoside Rg3 inhibits HIF-1α and VEGF expression in patient with acute leukemia via inhibiting the activation of PI3K/Akt and ERK1/2 pathways[J]. Int J Clin Exp Pathol, 2014, 7(5): 2172-2178.
[20]	Liu Q, Kou JP, Yu BY. Ginsenoside Rg1 protects against hydrogen peroxide-induced cell death in PC12 cells via inhibiting NF-κB activation[J]. Neurochem Int, 2011, 58(1): 119-125.
[21]	Guo CY, Zhang C, Xia ZM, et al. Nano-designed CO donor ameliorates bleomycin-induced pulmonary fibrosis via macrophage manipulation[J]. J Control Release, 2022, 341: 566-577.
[22]	Ciccolo JT, Carr LJ, Krupel KL, et al. The role of resistance training in the prevention and treatment of chronic disease[J]. Am J Lifestyle Med, 2010, 4(4): 293-308.
[23]	Yang K, Palm J, K?nig J, et al. Matrix-Metallo-Proteinases and their tissue inhibitors in radiation-induced lung injury[J]. Int J Radiat Biol, 2007, 83(10): 665-676.
[24]	José RJ, Williams AE, Chambers RC. Proteinase-activated receptors in fibroproliferative lung disease[J]. Thorax, 2014, 69(2): 190-192.
[25]	Betensley A, Sharif R, Karamichos D. A systematic review of the role of dysfunctional wound healing in the pathogenesis and treatment of idiopathic pulmonary fibrosis[J]. J Clin Med, 2016, 6(1): 2.
[26]	Gay SE, Kazerooni EA, Toews GB, et al. Idiopathic pulmonary fibrosis: predicting response to therapy and survival[J]. Am J Respir Crit Care Med,1998,157(4 Pt 1):1063-1072.

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稀有人参皂苷对特发性肺纤维化的影响

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Effects of rare ginsenoside on idiopathic pulmonary fibrosis

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