基于液相色谱-多级多反应监测模式（LC-MRM<sup>3</sup>）的高灵敏定量分析新策略

李婷; 张珂; 李军; 王胜鹏; 宋月林

doi:10.11665/j.issn.1000-5048.2023062801

基于液相色谱-多级多反应监测模式（LC-MRM³）的高灵敏定量分析新策略

李婷^1,2,
张珂^1,2,
李军^1,2,
王胜鹏³,
宋月林¹

1.
北京中医药大学北京中医药研究院，北京 102488
2.
北京中医药大学中药学院，北京 102488
3.
澳门大学中药质量研究国家重点实验室，澳门 999078

基金项目: 国家自然科学基金资助项目（No.81973444）；中药质量研究国家重点实验室（澳门大学）开放课题资助项目（No.SKL-QRCM-OP21011）

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出版历程
- 收稿日期: 2023-06-27
- 修回日期: 2023-12-06
- 刊出日期: 2023-12-24

An emerging analytical tool for highly sensitive quantitative analysis based on liquid chromatography-multiple-reaction monitoring cubed (MRM³)

1.
Beijing Research Institute of Chinese Medicine,Beijing University of Chinese Medicine,Beijing 102488
2.
School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488
3.
State Key Laboratory of Quality Research in Chinese Medicine,University of Macau,Macao 999078, China

Funds: This study was supported by the National Natural Science Foundation of China (No.81973444) and the Open Research Project Programme of the State Key Laboratory of Quality Research in Chinese Medicine (University of Macau) (No.SKL-QRCM-OP21011)

摘要

摘要: 液相色谱多级质谱联用（LC-MS/MS）的多反应监测模式（multiple-reaction monitoring，MRM）已经成为复杂体系中多成分同步定量分析的金标准，广泛应用于药品、中药、食品等各个领域。然而，由于选择性的限制，在对复杂基质中痕量化学成分定量时，MRM无法有效降低噪音或基质干扰，灵敏度不足。多级多反应监测模式（multiple-reaction monitoring cubed，MRM³）是对碰撞诱导解离（collision induced dissociation，CID）产生的子离子碎片进行富集并再次裂解，检测其孙离子碎片信号。通过多次离子筛选，显著提升了选择性和灵敏度，因此LC-MRM³得到了广泛关注。本文针对该方法的原理、参数设置及在生物标志物分析、药物分析、法医和毒物分析、食品分析和环境分析等领域中的应用进行介绍和系统综述，为利用LC-MRM³实现复杂基质中痕量化学成分的定量分析提供参考。
- MRM³ /
- 定量分析 /
- 复杂基质 /
- 灵敏度 /
- LC-MS/MS
Abstract: Liquid chromatography-multiple-reaction monitoring (LC-MRM) has been widely recognized as the golden standard for multiple components-targeted quantitative analysis of complicated matrices,with extensive applications for analysis in such fields as chemical drugs, traditional Chinese medicines and foods.Unfortunately, when facing the task of quantitatively analyzing trace chemical components in complex matrices, MRM suffers dramatically from the background noise or matrix interference, leading to undesirable sensitivity and selectivity in terms of the lower limits of quantification (LOQ) and detection (LOD).In recent years, MRM cubed (MRM³), also known as MS³ scan, has received much attention because of its unique ability to significantly improve detection selectivity and sensitivity attributing to the successive ion filtering function, enabling LC-MRM³as an emerging analytical tool.In this review,our attention is devoted to: 1) the illustration of the principle for MRM³; 2) parameter settings; and 3) the application progress of LC-MRM³ in such fields as the pursuit of biomarkers, pharmaceutical analysis, forensic analysis, toxicological analysis, food chemistry, and environmental analysis, aiming to provide a promising analytical tool of LC-MRM³ advantageous in the quantification analysis of trace chemical components in complex matrices.
- MRM³ /
- quantitative analysis /
- complex matrices /
- sensitivity /
- LC-MS/MS

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