Applications of ferritin-based delivery system in biomedical field
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摘要:
铁蛋白普遍存在于各种生物体内，负责储存过量铁以维持体内铁平衡。由于铁蛋白具有固有靶向性、天然空腔结构、可逆性自组装、高生物相容性及易被修饰等天然优势，被认为是一种理想的递送系统，广泛应用于多个领域。本文重点综述铁蛋白的生物学特性、功能化修饰、载药策略以及在药物递送、生物催化、光动力治疗、医学成像以及疫苗研究等生物医学领域中的研究进展和应用前景，为基于铁蛋白的递送系统在生物医学领域中的相关研究提供借鉴。
Abstract:Ferritin is widely present in various organisms and is responsible for storing excess iron to maintain iron balance in vivo. Due to its inherent targeting ability, natural cavity structure, reversible self-assembly, high biocompatibility, and easy modification, ferritin is considered to be an ideal delivery system, which is widely used in many fields. This review summarizes the biological characteristics, functionalization, drug loading strategies, research progress and application prospects of ferritin-based nanocarrier systems in biomedical fields, such as drug delivery, biocatalysis, photodynamic therapy, medical imaging and vaccine research, aiming to provide some reference for related biomedical research based on ferritin delivery systems.
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表 1 以铁蛋白作为递送系统的生物医学研究
领域 包载药物/表面修饰 铁蛋白类型 载药方法 功能应用 参考文献 药物载体 雷帕霉素和Erastin 铁蛋白 物理孵育 诱导肿瘤细胞铁死亡 [29] 光敏剂和表柔比星 马脾脏去铁蛋白 pH解聚/重组装 清除乳腺癌转移的CSCs [28] 阿霉素 热球菌铁蛋白 尿毒梯度 抑制肝癌生长及肺转移 [21] 核酸递送 siRNA 人去铁蛋白 pH解聚/重组装 免疫激活及抗炎 [35] siRNA 人重链铁蛋白 pH解聚/重组装 干扰RNA表达并抑制肿瘤生长 [36] TLR核酸配体 人铁蛋白突变体 pH解聚/重组装 抗肿瘤免疫治疗 [12] 多肽类药物 吞噬诱导肽SIRPα 铁蛋白 基因工程 肿瘤靶向治疗 [37] 多价凝块靶向肽 溶血栓铁蛋白 基因工程 靶向破坏血管中血栓 [38] 血管紧张素转换酶抑制肽 马脾脏铁蛋白 物理孵育 肠道靶向释放 [39] 生物催化 Fe3O4 人重链铁蛋白 温度通道 过氧化氢酶活性 [42] Co3O4 激烈火球菌铁蛋白 原位氧化 类过氧化物酶活性 [43] 光动力治疗 ZnF16Pc RGD修饰的铁蛋白 物理孵育 PDT治疗肿瘤 [47] ZnF16Pc FAP修饰的铁蛋白 pH解聚/重组装 选择性清除肿瘤部位CAF [48] 生物成像 68Ga 68Ga-NOTA-Tf 物理孵育 核成像 [51] 99mTc 人重链铁蛋白 物理孵育 SPECT和CT双模式成像 [52] IR820 铁蛋白 pH解聚/重组装 光声/荧光多模式成像 [54] 疫苗研发 SARs-CoV-2抗原 幽门螺杆菌铁蛋白 基因工程 SARs-CoV-2铁蛋白纳米疫苗 [57] SpyTag抗原 铁蛋白 点击连接 肿瘤个性化疫苗 [59] 甲型流感血凝素 幽门螺杆菌铁蛋白 基因工程 流感疫苗 [60] 
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