丹参愈伤组织化学成分的初步研究

Preliminary Investigation of Chemical Constituents in Callus Tissue of Danshen

摘要: 本文报道了丹参愈伤组织的诱导及其化学成分的初步研究。试验采用Murashige-Skoog(MS)培养基,分别加入2,4—二氯苯氧乙酸(2,4-D)1mg/l、β-吲哚乙酸(IAA)1mg/l、α--萘乙酸(NAA)1mg/l,在黑暗条件下培养三周后发现附加有 IAA 或 NAA 的培养基中愈伤组织变为红色。从干燥的红色愈伤组织中,用氯仿提取、硅胶柱层分离到一种橙红色结晶,经鉴定为隐丹酮。

Abstract: Callus tissue of Salvia miltiorrhiza Bge. can he used to biosynthesize diterpinequinones such as Tanshinone. The Callus tissue was grown on Murashige-Skoog (MS)medium containing 2,4-Dichlorophenoxyacetic acid ( 2,4-D ) 1mg/l or Indole aceticacid (IAA) 1mg/l or Naphthyl acetic acid ( NAA ) 1mg/l in the dark.It was found,after three weeks, that callus tissue growing on medium with IAA or NAA. turned red. An orange-red crystalline substance was isolated from the chloroform extract of red callusdry powder by silica column chromatography. It was found to be identical with cryptotanshinone by comparison with their mp, HPLC, UV and MS data.