紫外分光光度法和比色法测定具有邻苯二酚结构的药物的含量 Ⅳ.盐酸异丙肾上腺素及愈喘气雾剂的含量测定

UV Spectrophotometric and Colorimetric Determination of Ortho Dihydric phenolic Drugs Ⅳ. Determination of Isoprenaline Hydrochloride and It's Content in Aerosol

摘要: 应用紫外分光光度法和比色法测定了异丙肾上腺素。紫外分光光度法在波长279±1nm 处测定,吸收系数为 E_(1cm)~(1%)279nm=112.8。比色法基于在碱性条件下,亚硝酸钠—钼酸钠与异丙肾上腺素生成红色化合物,在波长500±1nm 处进行比色测定。浓度范围在4～40μg/ml 与吸收值呈线性。愈喘气雾剂中的异丙肾上腺素亦可用比色法测定含量,愈创木酚甘油醚和抗坏血酸无干扰。

Abstract: The ultraviolet spectrophotometric method and colorimetric method are used for thedetermination of isoprenaline hydrochlorid aerosol. The former is carried out bymeasuring absorbance at 279nm±mm. The absorption coefficient of isoprenaline isdetermined,E_(1cm)~(1%)279nm=112.8; The latter method is based on the reaction ofisoprenaline with sodium nitrite-sodium molybdate at alkaline medium forming a redcompound. By measuring the absorption of such red compound at 500±mm, it ispossible to determine the isoprenaline content in the sample. The concentration rangeof isoprenaline is between 4～40μg/ml, which is in agreement with Beer's laws.Theisoprenaline in the aerosol is also determined colorimetrically. Interferences to owning guaiphenesin and ascorbic acid are negligible.