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耐药性金黄色葡萄球菌青霉素酶的研究

STUDIES ON THE PENICILLINASE FROM ANTIBIOTIC-RESISTANT S.AUREUS

  • 摘要: 南京临床分离的耐药性金黄色葡萄球菌75号菌株产生细胞结合青霉素酶和胞外青霉素酶,但未能证明有细胞结合胞外青霉素酶。于对数生长期的培养物中加入苯唑青霉素,青霉素酶的产生率较低;如于培养基中加入柠檬酸钠,则笨唑青霉素对青霉素酶的诱导作用显著增加。以0.15M 柠檬酸钠对青霉素酶产生的刺激效果最显著。总酶和胞外酶的活力分别是未加柠檬酸钠培养基的8.7和16.9倍。柠檬酸钠的加入对细菌的生长有明显的抑制作用。用改良的 Richmond 方法纯化葡萄球菌胞外青霉素酶,平均总收率为25.33%,其平均比活力为21729单位/微克蛋白。

     

    Abstract: The cell-bound penicillinase and exopenicillinase were excreted by the S. aurcus75 strain isolated clinically in Nanjing, but no cell-bound exopenicillinase was demonstrated. When the exponentially growing cultures were induced by adding oxacillin alone, penicillinase produced was at a low rate in the cultures. However, if sodium citrate is placed in the culture medium before, the prduction of the penicillinase by oxacillin was increasd in a large amount in the cultures. When the concentration of the sodium citrate in the medium was 0. 15M the specific activity of the total penicillinase and the exopenicillinase in the cultures over a period of 18 hrs. was about 8.7 and 16.9 fold respectively as much as that in control.The staphylococcal exopenici1linase was purified by the modified Richmond's method, the total recovery being 25.33% and the specific activity being 2l729units/μg of protein.

     

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