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双波长分光光度法和吸收度比法在复方制剂分析中的应用Ⅱ.安钠咖注射液中咖啡因和苯甲酸钠的含量测定

Application of Dual Wavelength Spectrophotometry and Absorbance Ratio Method in Analysis of Compound Preparations Ⅱ.Determination of Caffeine and Sodium Benzoate in Caffeine and Sodium Benzoate Injection

  • 摘要: 用双波长分光光度法和吸收度比法不经分离、直接测定安钠咖注射液中咖啡因和苯甲酸钠的含量。其中双波长分光光度法同时用单波长分光光度计和双波长分光光度计两种仪器进行了试验。本法方法迅速,操作简便,咖啡因的平均回收率为98.81~100.3%,其变异系数小于0.59%;苯甲酸钠的平均回收率为98.44~100.7%,其变异系数小于0.60%。对四个厂家、五个批号的安钠咖注射液用本法进行了测定,其结果和采用中国药典(1977年版)法测定的结果进行了比较,准确度和精密度能够满足制剂分析的要求。

     

    Abstract: The dual wavelength spectrophotometry and the absorbance ratio method have been used for determination of caffeine and sodium benzoate in caffeine ard sodium benzoate injection without any preliminary separation.In the dual wavelength spectrophotometry both single ard dual wavelength spectrophotometers have been used.The methods are simple and rapid.Good results have been obtained. The averagerecovery of caffeine is 98.81-100.3% with coefficient of variation below 0.59%; the average recovery of sodium henzoate is 98.44-100.7% with coefficient of variation below 0.6O%. Five batches of sample from different factories have been determined with these methods. The results hare been compared with those obtained by the method of Chinese Pharmacopoeia ( 1977 ). The accuracy and precision meet the requiremrnt of analysis of the pharmaceutical preparations.

     

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